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| Registros recuperados: 114 | |
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| Ambrósio,Sandra Tereza; Melo,Natoniel Franklin de. |
| The present work reports the effect of different pH and sucrose concentrations on in vitro propagation of Nephrolepis biserrata. Fronds aseptically obtained from stolon segment culture were cultivated in MS semi-solid medium supplemented with 0, 15, 30, 45 and 60g.L-1 sucrose and pH adjusted to 3, 5, 7 and 9, in a factorial design. Frond number and length, pinnae number, raquis length and diameter, fresh and dry matter weight were measured. Inhibition of shoot and leaf regeneration was observed in all the pH treatments in the absence of sucrose. On the other hand, when sucrose was added to the medium, the shoot number increased, reaching the maximum average values of 51.25 and 38.25 shoot per explant at pH 5 and 7, respectively. Sucrose concentrations from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ferns; Ornamental plants; Tissue culture. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000400011 |
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| Assis, Kerlley Cristina; Empresa Brasileira de Pesquisa Agropecuária, Embrapa Clima Temperado; Pereira, Flávia Dionísio; Cooperativa Agroindustrial dos Produtores Rurais do Sudoeste Goiano; Cabral, Juliana Silva Rodrigues; Instituto Federal de Educação, Ciência e Tecnologia Goiano; Silva, Fabiano Guimarães; Instituto Federal de Educação, Ciência e Tecnologia Goiano; Silva, José Waldemar; Universidade Federal de Uberlândia; Santos, Silvia Correa dos; Universidade Federal de Goiás. |
| Anacardium othonianum Rizz. is a medicinal plant species that is native to the Brazilian savannah. Adult plants are different from other genus members in this ecosystem due to their size, and efforts to locate the plants may lead to their extraction from the savannah and, frequently, plant death. Micropropagation has played a significant role in the propagation and preservation of specimens of several plant species; therefore, this study evaluated the effect of various salt concentrations and culture medium volumes on the in vitro cultivation of A. othonianum Rizz. Trial (I) evaluated two culture media (MS and WPM) and three salt concentrations (100, 50 and 25%) in a completely randomized design. Trial (II) evaluated two culture media and salt... |
| Tipo: Pesquisa |
Palavras-chave: 5.01.03.04-0 cashew; Tissue culture; In vitro propagation Produção Vegetal. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10984 |
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| Brondani, Gilvano Ebling; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Araujo, Marla Alessandra; Universidade Federal de Lavras; Alcântara, Berenice Kussumoto; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Carvalho, Janice Guedes; Universidade Federal de Lavras; Gonçalves, Antonio Natal; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Almeida, Marcilio de; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP). |
| The in vitro organogenesis of woody species plays an essential role in the improvement of forest products by providing saplings with high commercial value. Furthermore, mineral nutrition plays an important role in the induction of organogenic responses. The objective of this study was to evaluate the effects of boron and calcium in the organogenesis of nodal segments from seedlings of Eucalyptus grandis growing under in vitro conditions. The concentration of boron and calcium in MS medium was modified to induce organogenic responses in 45-day-old nodal segments used as explants. After 60 days, the fresh weight, dry weight, ratio of fresh and dry weight, relative water content and relative matter content accumulated by the explants were evaluated. The... |
| Tipo: Pesquisa com fatores controlados |
Palavras-chave: 5.01.03.04-0 bud induction; Callogenesis; Tissue culture; Mineral nutrition 5.01.03.04-0 bud induction; Callogenesis; Tissue culture; Mineral nutrition. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15143 |
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| Schmildt, Edílson Romais; UFES; Amaral, José Augusto Teixeira do; UFES; Schmildt, Omar; UENF; Coelho, Ruimário Inácio; UFES; Rabello, Wanderson Souza; UENF; Martins Filho, Sebastião; UFV. |
| Este trabalho teve como objetivo avaliar a resposta rizogênica in vitro de ápices caulinares de plantas jovens de mamoeiro ‘Tainung 01’, em diferentes tempos de permanência em meios de indução e regeneração. O meio de indução empregado foi o MS, suplementado com complementos orgânicos constituídos de sacarose, mio-inositol, tiamina, piridoxina, ácido nicotínico, além de AIB. O meio de regeneração diferiu do meio de indução por ser desprovido de AIB. Os tratamentos consistiram em tempos variáveis de permanência dos explantes nos meios de indução e de regeneração (indução/regeneração): 0/25; 5/20; 10/15; 15/10; 20/5 e 25/0 dias. O delineamento experimental foi inteiramente casualisado, com seis tratamentos e quatro repetições, e cada unidade experimental foi... |
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Palavras-chave: 5.01.03.04-0 Produção de Mudas Carica papaya; Auxina; Micropropagação; Cultura de tecidos Carica papaya; Auxin; Micropropagation; Tissue culture. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/863 |
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| Silva, Marcus Vinícius Vieira da; Instituto Federal Goiano; Sales, Juliana de Fátima; Instituto Federal Goiano; Silva, Fabiano Guimarães; Instituto Federal Goiano; Neto, Aurélio Rubio; Instituto Federal Goiano; Alberto, Paula Sperotto; Instituto Federal Goiano; Pereira, Flávia Dionísio; IFGoiano - Campus Rio Verde. |
| The objective of this study was to evaluate the physiological quality of babassu (Orbignya phalerata Mart.) embryos as a function of desiccation. The fruits were placed in a convection oven at 37±2°C for 0, 3, 6, 9, or 12 days. After these periods, the moisture contents of the fruits and seeds were determined. A reduction in the water content occurred more rapidly in the fruits than in the seeds. The decrease in seed moisture content from 12.59% (on the basis of fresh weight) to 4.56% did not affect the percentage of germinated zygotic embryos; however, we did observe a decline in the vigor as the seed moisture content decreased. Embryos with a 4.56% moisture content developed into seedlings of shorter lengths after 30, 60, and 90 days of in vitro growth.... |
| Tipo: Pesquisa Empírica |
Palavras-chave: 5.01.03.00-8 Arecaceae; Desiccation tolerance; Tissue culture; Fitotecnia. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13429 |
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| Soares, Joyce Dória Rodrigues; Universidade Federal de Lavras; Pasqual, Moacir; Universidade Federal de Lavras; Rodrigues, Filipe Almendagna; Universidade Federal de Lavras; Villa, Fabiola; Universidade Federal de Lavras; Araujo, Aparecida Gomes de; Universidade Federal de Lavras. |
| O objetivo deste trabalho foi determinar a fonte de silício que proporcione melhor crescimento em plântulas de Cattleya loddigesii. Plântulas oriundas de sementes germinadas in vitro com aproximadamente 1,0 cm de comprimento foram inoculadas em tubos de ensaio contendo 15 mL de meio de cultura Knudson C modificado, acrescido de silicato de sódio (0, 5, 10 e 20 mg L-1) e silicato de potássio (0, 5, 10 e 20 mL L-1), em todas as combinações possíveis. O meio de cultura teve seu pH ajustado para 5,8 ± 0,1 e solidificado com 5 g L-1 de ágar antes do processo de autoclavagem a 121°C e 1 atm por 20 min. O cultivo foi mantido em sala de crescimento com irradiância de 35 µmol m-2 s-1, temperatura de 25 ± 1°C e... |
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Palavras-chave: 5.01.03.00-8 Cattleya loddigesii; Meio de cultura; Orchidaceae; Cultura de tecidos Fitotecnia/Biotecnologia Cattleya loddigesii; Culture medium; Orchidaceae; Tissue culture. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6281 |
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| Schmildt, Edílson Romais; UFES; Amaral, José Augusto Teixeira do; UFES; Schmildt, Omar; UENF; Coelho, Ruimário Inácio; UFES; Rabello, Wanderson Souza; UENF; Martins Filho, Sebastião; UFV. |
| Este trabalho teve como objetivo avaliar níveis de AIB no enraizamento in vitro de microestacas de mamoeiro (Carica papaya L. ‘Tainung 01’). Ápices caulinares de plantas com 120 dias de idade foram estabelecidos e avaliados em meio MS, suplementado com complementos orgânicos constituídos de sacarose a 87,64 mM, mio-inositol a 555,06 µM, tiamina-HCl a 0,30 µM, piridoxina-HCl a 2,43 µM, ácido nicotínico a 4,06 µM e 0; 0,74; 1,48; 2,96 e 5,92 µM de AIB, em delineamento inteiramente casualizado, com quatro repetições e cinco tubos de ensaio por parcela. Após 30 dias, avaliou-se a percentagem de enraizamento, o crescimento das microestacas, o comprimento da maior folha e a massa de calo. Observou-se que o maior percentual estimado de enraizamento ocorreu com a... |
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Palavras-chave: 5.01.00.00-9 Agronomia Carica papaya; Auxina; Micropropagação; Cultura de tecidos Fitotecnia Carica papaya; Auxin; Micropropagation; Tissue culture. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/866 |
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| Castro, Juliana Pereira de; Universidade Federal da Paraíba; Araújo, Emmanuelle Rodrigues; Universidade Federal da Paraíba - UFPB; Rêgo, Mailson Monteiro do; Universidade Federal da Paraíba - UFPB; Rêgo, Elizanilda Ramalho do; Universidade Federal da Paraíba - UFPB. |
| This work aimed to promote in vitro seed germination and disinfestation of the sweet cactus. Seeds were submerged in alcohol at 70% for 1 min. and then treated with sodium hypochlorite solution at different concentrations: 0.0; 0.5; 1.0 and 1.5%. After this treatment, the seeds were washed four times in distillated, deionized and autoclaved water. Seeds were inoculated in MS medium with different concentrations of sucrose (0.0, 2.5, 5.0, 7.5 and 10.0 g L-1). The experimental design was completely randomized in a factorial experiment 4 x 5, with five replicates for treatments. Contamination, germination and seedling growth were evaluated. The results of the analysis of variance indicate that there were no significant interactions among factors. There was no... |
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Palavras-chave: 5.01.00.00-9 Cactaceae; Forage; Tissue culture 5.01.00.00-9 Cactaceae; Forage; Tissue culture. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6275 |
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| Braga,Virgínia F.; Mendes,Giselle C.; Oliveira,Raphael T.R.; Soares,Carla Q.G.; Resende,Cristiano F.; Pinto,Leandro C.; Santana,Reinaldo de; Viccini,Lyderson F.; Raposo,Nádia R.B.; Peixoto,Paulo H.P.. |
| This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 µM)/α-naphthaleneacetic acid (NAA; 0.005 µM) induced multiple shoots. Elongated shoots were rooted with IAA (0.2 µM). Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF) of powdered leaves presented a radical scavenging activity with IC50 = 169.3 µg mL-1. HF showed a non-dose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Biodiversity conservation; Biological effects; Natural products. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100014 |
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| PáDUA,MARLÚCIA S.; PAIVA,LUCIANO V.; LABORY,CLAÚDIA R.G.; ALVES,EDUARDO; STEIN,VANESSA C.. |
| ABSTRACTOil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses inE.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryogenic potential; Growth regulators; Tissue culture; Transmission electron microscopy. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401545 |
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| GHATPANDE,SATISH; SHAFIQ,SAIYID; SIDDIQUI,M.A.Q.. |
| Recent gene knock-out studies in mice have suggested that ventricular myosin light chain-2 (vMLC2) has a role in the regulation of cardiogenic development and that perturbation in expression of vMLC2 is linked to the onset of dilated cardiomyopathy. In an attempt to develop an avian model for such studies, we examined the expression pattern of vMLC2 in chicken embryos at various stages and analyzed the effect of antisense oligonucleotide-mediated interference of vMLC2 function in cultures of whole embryos. Our results showed vMLC2 to be a specific marker for ventricular chamber throughout chicken embryonic development and antisense vMLC2 treatment of primitive streak stage (stage 4) embryos to produce pronounced dilation of heart tube with severe... |
| Tipo: Journal article |
Palavras-chave: Cardiogenesis; Cardiomyopathy; Myosin light chains; Tissue culture. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100009 |
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| Takemori,N. K.; Marschner,R.; Quoirin,M.; Bona,C.; Zanette,F.. |
| In order to achieve adventitious shoot regeneration from young tissues of Racosperma mangium, several combinations of growth regulators were tested. Kinetin (KIN) and a -naphthalenacetic acid (NAA), thidiazuron (TDZ) and NAA or indole-3-butyric acid (IBA), 4-CPPU and 2,4-dichlorophenoxyacetic acid (2,4-D) were added to basic medium composed of MS salts, MS vitamins and 3% sucrose. Sections of hypocotyls and cotyledons of young seedlings were cultivated on these media. Calli developed on different media and, after two to three months, some of them regenerated organized structures. Histological studies revealed hypertrophy and hyperplasy of medular and cortical parenchyma cells of hypocotyl and cotyledon parenchyma. Formation of meristematic cells and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 4-CPPU; Leguminosae; TDZ; Tissue culture. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100008 |
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| Registros recuperados: 114 | |
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