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| Asakuma, Sasaki; Yokoyama, Tomoko; Kimura, Kazumasa; Watanabe, Yoko; Nakamura, Tadashi; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡. |
| It is believed that human milk oligosaccharides (HMO) act as receptor analogues that inhibit the attachment of pathogenic microorganisms to the infant colon, but the direct involvement of HMO in anti infection has not as yet been demonstrated. The present study was conducted to clarify whether HMOs as well as commercially available galacto oligosaccharides (GO), from which lactose has been removed, affect the expression of toll-like receptor (TLR) 2 and 4, and of MD2 mRNA in the HT-29, human colonic cell line. HT-29 cells were treated with neutral human milk oligosaccharides (nHMOs), which had been separated from the acidic oligosaccharide fraction and also from lactose, as well as with GOs. HT-29 cells were treated with specific oligosaccharides of major... |
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Palavras-chave: Human milk oligosaccharides; Galacto-oligosaccharides; Toll-like receptor; Intestinal cell; HT-29 cells. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4184 |
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| Lee,HY; Biswas,D; Ahn,J. |
| ABSTRACT This study was designed to assess the role of bacteriophage P22 in the adhesion, invasion, intracellular survival of, and cellular immune response to Salmonella Typhimurium in intestinal epithelial INT-407 and chicken macrophage-like HD11 cells. The ability of S. Typhimurium to adhere, invade, and survive to INT-407 and HD11cells was evaluated under Salmonella infection alone (control), phage treatment followed by Salmonella infection (PS), Salmonella infection followed by phage treatment (SP), and a combination treatment with Salmonella and phage (S+P). The number of S. Typhimurium associated on INT-407 cells was reduced from 4.2 to 2.7 log cfu/cm2 by phage treatment (SP). The number of intracellular S. Typhimurium within INT-407 cells was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adhesion; Bacteriophage; Invasion; Salmonella; Toll-like receptor. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400427 |
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