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| Registros recuperados: 22 | |
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| BITTENCOURT-OLIVEIRA,MARIA DO CARMO; PICCIN-SANTOS,VIVIANE; MOURA,ARIADNE N.; ARAGÃO-TAVARES,NÍSIA K.C.; CORDEIRO-ARAÚJO,MICHELINE K.. |
| Brazil has a history of blooms and contamination of freshwater systems by cyanobacterial toxins. The monitoring relevance of toxins from cyanobacteria in reservoirs for public supply is notorious given its high toxicity to mammals, included humans beings. The most recurrent toxins in Brazilian water bodies are microcystins (MC). However, the recent record of cylindrospermopsin (CYN) in northeastern Brazil, Pernambuco state, alerts us to the possibility that this could be escalating. This study reports occurrence of MC and CYN, quantified with ELISA, in 10 reservoirs, devoted to public drinking supply in northeastern Brazil. The composition and quantification of the cyanobacteria community associated with these water bodies is also presented. From 23... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bloom; Harmful algae; Monitoring; Toxin; Water quality. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000100297 |
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| Samuels,Richard I.. |
| Destruxin A, a toxic cyclodepsipeptide, was purified from culture filtrates of the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorok. by Reverse-Phase HPLC, and assayed for its effects on a larval Manduca sexta (L.) (Lepidoptera: Sphingidae) heart preparation. Destruxin A was found to cause reversible and dose-dependent acceleration of the heart beat. The heart bioassay was highly sensitive to pulse applications of destruxin A at concentrations as low as 35 pmol. Because of its high level of sensitivity, the M. sexta heart bioassay could be used to detect and quantify destruxins in tissue extracts from mycosed insects or to confirm bioactivity of HPLC fractions. The site of action of Destruxin A was not determined. However, this toxin did not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Manduca sexta; Heart; Toxin; Peptide; Destruxin. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000200009 |
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| Costa,M.M.; Drescher,G.; Maboni,F; Weber,S.S.; Schrank,A.; Vainstein,M.H.; Schrank,I.S.; Vargas,A.C.. |
| Virulence factors and antimicrobial resistance patterns of Escherichia coli isolates were evaluated. A total of 80 E. coli isolates were evaluated, being 64 from clinical samples (intestinal content and fragments of organs from diarrheic piglets), seven from feces of clinically healthy piglets and sows, and nine environmental samples (five from facilities, two from feed, one from insect, and one from waste). Molecular characterization was performed by PCR detection of fimbriae and toxin genes and plasmid content determination. The isolates were also characterized according to their resistance or sensitivity to the following drugs: ampicillin, trimethoprim:sulfamethoxazole, tetracycline, amikacine, colistin, norfloxacin, florfenicol, enrofloxacin,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swine; Colibacillosis; Fimbriae; Toxin; Antimicrobial resistance. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000100004 |
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| Ugur,Hacer Gok; Sıralı,Recep; Tekgul,Ahmet Talha; Efe,Burcin. |
| Abstract This study assessed mad honey use in alternative treatments. The universe of this descriptive study was patients admitted to the pulmonary disease clinic located in the Ordu province of the Black Sea region between 15 December 2014 and 15 February 2015. We did not use a sampling method and patients who agreed to participate were included in the study (n=353). In order to collect the data, we used a questionnaire prepared by the researchers. In this study, 77% of the participants stated that mad honey was beneficial to health, 44.5% used mad honey, and 53.5% consumed it daily. Furthermore, 28.7% used mad honey for asthma, 6.4% for cough, 12.1% for gastrointestinal diseases, and 3.2% for hypertension. There was a significant relationship between the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mad honey; Health; Alternative treatment; Toxin; Poisoning. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100310 |
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| Alves,Guilherme Guerra; Machado-de-Ávila,Ricardo Andrez; Chávez-Olórtegui,Carlos Delfin; Silva,Rodrigo Otávio Silveira; Lobato,Francisco Carlos Faria. |
| Abstract The epsilon toxin, produced by Clostridium perfringens, is responsible for enterotoxemia in ruminants and is a potential bioterrorism agent. In the present study, 15 regions of the toxin were recognized by antibodies present in the serum, with different immunodominance scales, and may be antigen determinants that can be used to formulate subunit vaccines. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epsilon; Toxin; Enterotoxemia; Epitope; Mapping. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300570 |
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| Рябушко, Л. И.; Бесиктепе, С.; Едигер, Д.; Илмаз, Д.; Зенгинер, A.; Рябушко, В. И.; Ли, Р. И.. |
| В Чёрном море в прибрежных водах Крыма впервые обнаружена диатомовая водоросль Pseudo-nitzschia calliantha Lundholm, Moestrup et Hasle, продуцирующая домоевую кислоту. Концентрация токсина в клетках водоросли, культивируемой в питательных средах F/2 и Гольдберга, составляет в среднем соответственно 0.43 и 0.145 пг·кл.-1. Рассмотрены морфология, систематика, экология и токсикологические аспекты вида. |
| Tipo: Journal Contribution |
Palavras-chave: Диатомовая водоросль; Pseudo-nitzschia; Морфология; Токсин; Домоевая кислота; Крым; Чёрное море; Diatom; Morphology; Toxin; Domoic acid; Crimea; Black Sea; Діатомова водорість; Морфологія; Домоєва кислота; Крим; Чорне море. |
| Ano: 2008 |
URL: http://repository.ibss.org.ua/dspace/handle/99011/821 |
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| Belin, Catherine; Amzil, Zouher. |
| Toxin monitoring is carried out along the coasts of France by a national network, REPHY, which is the National Monitoring Network for Phytoplankton and Phycotoxins. Sampling strategy differs according to the type of zone coastal or offshore and to the family of toxins. For PSP and ASP toxins, monitoring is based on the detection of toxic phytoplankton species. For lipophilic toxins, a systematic analysis of toxins is performed in risk areas during high risk periods. Experimental monitoring for palytoxins has also been conducted along the Mediterranean coast since 2007. The total number of toxin analyses is in the order of 2500 to 3000 per year. The detailed results for each family of toxins showed that the most frequent toxic events for the period from... |
| Tipo: Text |
Palavras-chave: Monitoring; Phytoplankton; Toxin; Sampling; Shellfish; French coasts. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00077/18821/16398.pdf |
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| Rolland, Jean-luc; Medhioub, Walid; Vergnes, Agnes; Abi-khalil, Celina; Savar, Veronique; Abadie, Eric; Masseret, Estelle; Amzil, Zouher; Laabir, Mohamed. |
| To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in... |
| Tipo: Text |
Palavras-chave: Shellfish; Toxin; Biomarker; Expression; Phytoplankton. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00225/33606/32003.pdf |
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| Lambert, Christian; Nicolas, Jean-louis; Bultel, Valérie. |
| Using a chemiluminescence (CL) test, it had been previously demonstrated that Vibrio pectenicida, which is pathogenic to Pecten maximus larvae, was able to inhibit completely the CL activity of P, maximus hemocytes and partially inhibit those of Crassostrea gigas, Conversely, a Vibrio sp, strain, S322, pathogenic to C,gigas larvae was more active in reducing the CL activity of oyster hemocytes than of scallop hemocytes, Using this same CL biotest, V, pectenicida and S322 cytoplasmic extracts were shown to reproduce CL inhibition while the cytoplasmic extract of a nonpathogenic strain (U1, Pseudoalteromonas) was without effect. Moreover, cytoplasmic extract as well as live V, pectenicida cells provoked, within a few hours, death of P. maximus hemocytes... |
| Tipo: Text |
Palavras-chave: Bivalves; Hemocyte; Toxin; Chemiluminescence; Crassostrea gigas; Pecten maximus; Vibrio pectenicida. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-466.pdf |
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| Rubio, Tristan; Oyanedel, Daniel; Labreuche, Yannick; Toulza, Eve; Luo, Xing; Bruto, Maxime; Chaparro, Cristian; Torres, Marta; De Lorgeril, Julien; Haffner, Philippe; Vidal-dupiol, Jeremie; Lagorce, Arnaud; Petton, Bruno; Mitta, Guillaume; Jacq, Annick; Le Roux, Frederique; Charriere, Guillaume; Destoumieux-garzon, Delphine. |
| Vibrio species cause infectious diseases in humans and animals, but they can also live as commensals within their host tissues. How Vibrio subverts the host defenses to mount a successful infection remains poorly understood, and this knowledge is critical for predicting and managing disease. Here, we have investigated the cellular and molecular mechanisms underpinning infection and colonization of 2 virulent Vibrio species in an ecologically relevant host model, oyster, to study interactions with marine Vibrio species. All Vibrio strains were recognized by the immune system, but only nonvirulent strains were controlled. We showed that virulent strains were cytotoxic to hemocytes, oyster immune cells. By analyzing host and bacterial transcriptional... |
| Tipo: Text |
Palavras-chave: T6SS; Toxin; Dual RNA-seq; Cytolysis; Pathogenesis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00507/61837/65900.pdf |
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| Abikhalil, Celina; Finkelstein, Darren S.; Conejero, Genevieve; Du Bois, Justin; Destoumieux-garzon, Delphine; Rolland, Jean-luc. |
| Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, including saxitoxin, were also shown to be directly responsible for inducing apoptosis in hemocytes, a process dependent on caspase activation and independent of reactive oxygen species (ROS) production. A series of in vitro labeling and microscopy experiments revealed that STX and analogs there of induced nuclear condensation, phosphatidylserine exposure, membrane permeability, and DNA fragmentation of hemocytes. Unlike in vertebrates, gonyautoxin-5 (GTX5), which... |
| Tipo: Text |
Palavras-chave: Shellfish; Toxin; Harmful algae; Phytoplankton. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00391/50272/50905.pdf |
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| BACH,E. E.; KIMATI,H.. |
| Low molecular weight metabolites produced by Bipolaris bicolor, Bipolaris sorokiniana, and Drechslera tritici-repentis are considered to be toxins that facilitate disease in wheat cultivars. Several such toxins were isolated from these fungi. Electrophoresis demonstrated bands of proteins that reduced shoot inhibition in susceptible plants but not in resistant plants. Chlorophyll content was reduced during the first 10 hours of light in the susceptible plants and after 18 hours in the resistant plants. The enzyme beta-1,3-glucanase increased in the resistant plants after treatment with toxins, but in the susceptible plants this enzyme decreased compared to the control. This suggests that the toxin was a protein and the susceptible plants needed other... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxin; Wheat pathogens; Protein toxin. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301999000200006 |
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| Yonamine,C. M.; Costa,H.; Silva,J. A. A.; Muramoto,E.; Rogero,J. R.; Troncone,L. R. P.; Camillo,M. A. P.. |
| The use of radiotracers allows the understanding of the bioavailability process, biodistribution, and kinetics of any molecule labelled with an isotope, which does not alter the molecule's biological properties. In this work, technetium-99m and iodine-125 were chosen as radiotracers for biodistribution studies in mice using bee (Apis mellifera) venom and a toxin (PnTX2-6) from the Brazilian "armed" spider (Phoneutria nigriventer) venom. Incorporated radioactivity was measured in the blood, brain, heart, lung, liver, kidney, adrenal gland, spleen, stomach, testicle, intestine, muscle, and thyroid gland. Results provided the blood kinetic parameter, and different organs distribution rates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phoneutria nigriventer; Apis mellifera; Toxin; Venom; Radiotracer; Biodistribution. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100006 |
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| Erdeş,Efe; Doğan,Tuğba Somay; Coşar,İlhan; Danışman,Tarık; Kunt,Kadir Boğaç; Şeker,Tamay; Yücel,Meral; Özen,Can. |
| Background Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs.Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims.Methods In the present study, the peptide profile of the venom was investigated by electrophoretic methods, size-exclusion and reversed-phase chromatography and mass spectroscopy. Cytotoxic and antimicrobial effects were evaluated on a breast cancer cell line (MCF-7) and various bacterial and fungal species.Results Proteins make up approximately half of the dry weight of L. abdullahbayrami crude venom. Microfluidic capillary electrophoresis indicated the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scorpion venom; Toxin; Peptide; Leiurus abdullahbayrami; Microfluidic capillary; Electrophoresis; Peptidomics; Venomics; Cytotoxicity; Antimicrobial activity; Turkey. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200338 |
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| Clement,Herlinda; Flores,Vianey; la Rosa,Guillermo De; Zamudio,Fernando; Alagon,Alejandro; Corzo,Gerardo. |
| Abstract Background The cysteine-rich neurotoxins from elapid venoms are primarily responsible for human and animal envenomation; however, their low concentration in the venom may hamper the production of efficient elapid antivenoms. Therefore, the aim of the present study was to produce fully active elapid neurotoxic immunogens for elapid antivenom production. Method Cysteine-rich neurotoxins showed recombinant expression in two strains of E. coli, and were purified using affinity chromatography and reverse-phase HPLC (rpHPLC). Results The cDNA of the four disulfide-bridged peptide neurotoxin Mlat1 was cloned into a modified expression vector, pQE30, which was transfected into two different E. coli strains. The recombinant toxin (HisrMlat1) was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micrurus laticorallis; Protein folding; Recombinant; Elapid; Toxin; Protein recognition. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100318 |
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| Registros recuperados: 22 | |
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