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	Provedor de dados BJMBR (2) Autor Rodrigues,M.M. (2) Boscardin,S.B. (1) Costa,F. (1) Hiyane,M.I. (1) Pereira-Chioccola,V.L. (1) Ribeirão,M. (1) Schenkman,S. (1) Mais... Palavra-chave Trans-sialidase (2) Chagas' disease (1) DNA vaccination (1) NPA-PCR method (1) T-cell receptor (1) Trypanosoma cruzi (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2006 (1) 1999 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Trans-sialidase delivered as a naked DNA vaccine elicits an immunological response similar to a Trypanosoma cruzi infection BJMBR Costa,F.; Pereira-Chioccola,V.L.; Ribeirão,M.; Schenkman,S.; Rodrigues,M.M.. Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, does not synthesize sialic acid, but expresses a trans-sialidase (TS) that catalyzes the transfer of sialic acid from host glycoconjugates to the parasite surface. Here, we review studies that characterize the immune response to the catalytic domain of the enzyme in humans during Chagas' disease or in mice following immunization with the TS gene. In both cases, there are antibodies that strongly inhibit the enzymatic activity and generation of interferon-<FONT FACE="Symbol">g</FONT>-producing T cells. Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA vaccination; Trans-sialidase; Chagas' disease. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200013 The non-palindromic adaptor-PCR method for the identification of the T-cell receptor genes of an interferon-gamma-secreting T-cell hybridomaspecific for trans-sialidase, an immunodominant Trypanosoma cruzi antigen BJMBR Hiyane,M.I.; Boscardin,S.B.; Rodrigues,M.M.. Cloning of the T-cell receptor genes is a critical step when generating T-cell receptor transgenic mice. Because T-cell receptor molecules are clonotypical, isolation of their genes requires reverse transcriptase-assisted PCR using primers specific for each different Valpha or Vß genes or by the screening of cDNA libraries generated from RNA obtained from each individual T-cell clone. Although feasible, these approaches are laborious and costly. The aim of the present study was to test the application of the non-palindromic adaptor-PCR method as an alternative to isolate the genes encoding the T-cell receptor of an antigen-specific T-cell hybridoma. For this purpose, we established hybridomas specific for trans-sialidase, an immunodominant Trypanosoma... Tipo: Info:eu-repo/semantics/article Palavras-chave: NPA-PCR method; T-cell receptor; Trypanosoma cruzi; Trans-sialidase. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300005 Registros recuperados: 2 Primeira ... 1 ... Última

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