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Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.) Genet. Mol. Biol.
Yaish,M.W.F.; Pérez de la Vega,M..
The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA)n microsatellites with n values equal or higher than six. These (GA)n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA)n microsatellite linked to a putative MADS-box gene was identified. This sequence, which...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris L.; Genetic markers; Microsatellites; MADS-box; Transcriptional factor.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300019
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