Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: 

RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 26
Primeira ... 12 ... Última
Imagem não selecionada

Imprime registro no formato completo
Effects of high proline accumulation on chloroplast and mitochondrial ultrastructure and on osmotic adjustment in tobacco plants Agronomy
Borgo, Lucélia; Marur, Celso Jamil; Vieira, Luiz Gonzaga Esteves.
Proline accumulates in many organisms in response to abiotic stresses, such as drought and salinity. Reports have discussed the role of proline in relation to osmotic adjustment, even though no clear-cut evidence has yet been provided demonstrating this association as a defense mechanism against drought stress in plants. However, it has been indicated that the application of exogenous proline can damage the ultrastructure of chloroplasts and mitochondria. In this study, using transgenic tobacco plants carrying a mutant p5cs gene from Vigna aconitifolia under control of the CaMV 35S promoter, we show that the high levels of endogenous proline accumulated in leaves does not contribute to the osmotic adjustment in plants under water deficit conditions....
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.00.00-9 p5cs; Transgenic; Water deficit Melhoramento Vegetal.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19097
Imagem não selecionada

Imprime registro no formato completo
A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach Anais da ABC (AABC)
Alves-Costa,Fernanda A.; Figueiredo,Márcio A.; Lanes,Carlos F.C.; Almeida,Daniela V.; Marins,Luis F.; Wasko,Adriane P..
The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential display; GH; Growth hormone; Transgenic; Zebrafish.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200017
Imagem não selecionada

Imprime registro no formato completo
An improved method for transformation of lettuce by Agrobacterium tumefaciens with a gene that confers freezing resistance BABT
Pileggi,Marcos; Pereira,Albanin Aparecida Mielniczki; Silva,Joandrei dos Santos; Pileggi,Sônia Alvim Veiga; Verma,Desh Pal S..
An efficient method for constructing transgenic lettuce cultivars by Agrobacterium tumefaciens was described by Torres et al., 1993. In the present work, an improvement of the above procedure is described and applied to transform the cultivar Grand Rapids with a mutated P5CS gene. The major modifications were concerned with turning more practical the transformation and regeneration protocols. Also we tried to improve transformation steps by increasing injured area in explants and prolonging co-cultivation with Agrobacteria (in larger concentration). A more significant selective pressure was used against non-transformed plants and bacteria. In these work we were concerned to obtain T1 and T2 seeds. The P5CS gene codes for a delta¹-pyrroline-5-carboxylate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stress resistance; Cold resistance; Transgenic; Lettuce; Proline.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200013
Imagem não selecionada

Imprime registro no formato completo
Transgenic animal models for the functional analysis of vasoactive peptides BJMBR
Bader,M..
The interplay of vasoactive peptide systems is an essential determinant of blood pressure regulation in mammals. While the endothelin and the renin-angiotensin systems raise blood pressure by inducing vasoconstriction and sodium retention, the kallikrein-kinin and the natriuretic-peptide systems reduce arterial pressure by eliciting vasodilatation and natriuresis. Transgenic technology has proven to be very useful for the functional analysis of vasoactive peptide systems. As an outstanding example, transgenic rats overexpressing the mouse Ren-2 renin gene in several tissues become extremely hypertensive. Several other transgenic rat and mouse strains with genetic modifications of components of the renin-angiotensin system have been developed in the past...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Renin-angiotensin system; Transgenic; Animal models; Knockout.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900011
Imagem não selecionada

Imprime registro no formato completo
Genetic transformation of coffee Braz. J. Plant Physiol.
Ribas,Alessandra Ferreira; Pereira,Luiz Filipe Protasio; Vieira,Luiz Gonzaga E..
In the last 15 years, considerable advances were made in coffee genetic transformation. Different research groups in the world have been able to transform coffee with genes for insect resistance, decaffeinated coffee, herbicide resistance and control of fruit maturation. Although the majority of the research is still limited to laboratory and greenhouse studies, initial field tests with transformed coffee are beginning to appear in the literature. In this review we provide an update on the state of coffee genetic transformation, presenting technical aspects related to tissue culture systems, strategies for selection and transformation with particle bombardment, as well as the use of Agrobacterium tumefaciens. We also discuss the potential applications of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium; Coffea; Biolistic; Genetic engineering; Transgenic.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100007
Imagem não selecionada

Imprime registro no formato completo
Advances in Apple Transformation Technology to Confer Resistance to Fungal Diseases in Apple Crops: A Chilean Perspective Chilean J. Agric. Res.
Polanco,Víctor; Paredes,Mario; Becerra,Viviana; Pérez,Eduardo.
Apple (Malus domestica Borkh.) is one of the most consumed fruit in the world. Genetic transformation is a key process to sustain this demand by permitting the potential enhancement of existing cultivars as well as the development of new cultivars resistant to pests, diseases, and storage problems that occur in the major production areas. This review summarizes the advances of genetic engineering applied to the development of resistant apple cultivars to fungus disease, with particular attention in the generation of apples resistant to Venturia inaequalis (Cooke) G.Winter, the main phytosanitary problem that affects apple crops in Chile.
Tipo: Journal article Palavras-chave: Apple; Fungal disease; Transgenic; Cisgenesis.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200013
Imagem não selecionada

Imprime registro no formato completo
Attitudes towards genomic: Research in four Latin American countries Electron. J. Biotechnol.
Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando.
The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,...
Tipo: Journal article Palavras-chave: Cloning; Genome; Social representations; Transgenic.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003
Imagem não selecionada

Imprime registro no formato completo
A practical approach to the understanding and teaching of RNA silencing in plants Electron. J. Biotechnol.
Bazzini,Ariel A; Mongelli,Vanesa C; Hopp,H. Esteban; del Vas,Mariana; Asurmendi,Sebastián.
Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach...
Tipo: Journal article Palavras-chave: Gene silencing; GFP; HC-Pro; PDS; Protocols; PTGS; Teaching; Transgenic; Transient expression; TRV; VIGS.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002
Imagem não selecionada

Imprime registro no formato completo
Genetic approaches for studying transgene inheritance and genetic recombination in three successive generations of transformed tobacco Genet. Mol. Biol.
Tizaoui,Kalthoum; Kchouk,Mohamed Elyes.
Transgene integration into plant genomes is a complex process accompanied by molecular rearrangements. Classic methods that are normally used to study transgenic population genetics are generally inadequate for assessing such integration. Two major characteristics of transgenic populations are that a transgenic genome may harbor many copies of the transgene and that molecular rearrangements can create an unstable transgenic locus. In this work, we examined the segregation of T1, T2 and T3 transgenic tobacco progenies. Since transfer DNA (T-DNA) contains the NptII selectable marker gene that confers resistance to kanamycin, we used this characteristic in developing a method to estimate the number of functional inserts integrated into the genome. This...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copy number variation; Gene rearrangements; Genetic inheritance; Transgenic.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400015
Imagem não selecionada

Imprime registro no formato completo
Chitinase activities, scab resistance, mycorrhization rates and biomass of own-rooted and grafted transgenic apple Genet. Mol. Biol.
Schäfer,Tina; Hanke,Magda-Viola; Flachowsky,Henryk; König,Stephan; Peil,Andreas; Kaldorf,Michael; Polle,Andrea; Buscot,François.
This study investigated the impact of constitutively expressed Trichoderma atroviride genes encoding exochitinase nag70 or endochitinase ech42 in transgenic lines of the apple cultivar Pinova on the symbiosis with arbuscular mycorrhizal fungi (AMF). We compared the exo- and endochitinase activities of leaves and roots from non-transgenic Pinova and the transgenic lines T386 and T389. Local and systemic effects were examined using own-rooted trees and trees grafted onto rootstock M9. Scab susceptibility was also assessed in own-rooted and grafted trees. AMF root colonization was assessed microscopically in the roots of apple trees cultivated in pots with artificial substrate and inoculated with the AMF Glomus intraradices and Glomus mosseae. Own-rooted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apple; Chitinase; Mycorrhiza; Transgenic.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300014
Imagem não selecionada

Imprime registro no formato completo
Ensaio de competição de cultivares tolerantes ao glifosato da rede soja sul de pesquisa, safra 2009/10. Infoteca-e
BERTAGNOLLI, P. F.; COSTAMILAN, L. M.; NICOLAU, M.; VERNETTI JUNIOR, F. de J.; STECKLING, C.; ROVERSI, T.; RUBIN, S. de A. L.; GONÇALVES, J. A.; CASTRO, R. L. de; GABE, N. L.; OLIVEIRA, M. A. R. de; VICENTE, D.; MATZENBACHER, R.; GIASSON, N. F.; BAGATINI, N. P.; SILVA, A. E. L. da.
Tipo: Parte de livro Palavras-chave: Soybean; Transgenic.
Ano: 2010 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/882340
Imagem não selecionada

Imprime registro no formato completo
Assessing the effects of genotype-by-environment interaction on epigenetic, transcriptomic, and phenotypic response in a Pacific salmon ArchiMer
Christensen, Kris A; Le Luyer, Jeremy; Chan, Michelle T T; Rondeau, Eric B; Koop, Ben F; Bernatchez, Louis; Devlin, Robert H.
Genotype-by-environment (GxE) interactions are non-parallel reaction norms among individuals with different genotypes in response to different environmental conditions. GxE interactions are an extension of phenotypic plasticity and consequently studying such interactions improves our ability to predict effects of different environments on phenotype as well as the fitness of genetically distinct organisms and their capacity to interact with ecosystems. Growth hormone transgenic coho salmon grow much faster than non-transgenics when raised in tank environments, but show little difference in growth when reared in nature-like streams. We used this model system to evaluate potential mechanisms underlying this growth rate GxE interaction, performing RNA-seq to...
Tipo: Text Palavras-chave: Epigenetics; Transcriptome; GxE; Transgenic; Growth hormone; Salmonid.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00677/78890/81188.pdf
Imagem não selecionada

Imprime registro no formato completo
Histocytological examination on organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant Phyton
Guan,Z-J; Guo,B; Huo,Y-L; Dai,J-K; Wei,Y-H.
The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige & Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron microscopy indicated that there were significant changes in morphology and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Histocytological; Organogenesis; Somatic embryogenesis; HBsAg; Transgenic; Cherry tomato; Mutant.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100008
Imagem não selecionada

Imprime registro no formato completo
The role of glyphosate in RR soybean production and seed quality Planta Daninha
Albrecht,L.P.; Albrecht,A.J.P.; Braccini,A.L.; Oliveira Jr.,R.S.; Zobiole,L.H.S.; Ávila,M.R..
This study aimed to evaluate the production components and quality of RR soybean seeds (Roundup Ready®), after application of increasing rates of gliphosate. Field experiments were conducted in Mandaguari, Paraná, during two seasons. Treatments consisted of five doses of glyphosate. All applications were performed once, between development stages V4 and V5. The experiment was arranged in a completely randomized block design, with four replicates. Data were subjected to analysis of variance, and when significant, t-tests and a regression analysis were applied to verify the behavior of the treatments. The physiological and sanitary quality, yield and mass of one thousand seeds were evaluated. The results indicated that seed quality can be adversely affected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Herbicide; Transgenic.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582014000200018
Imagem não selecionada

Imprime registro no formato completo
ISSUES IN DEVELOPMENT AND ADOPTION OF GENETICALLY MODIFIED (GM) WHEATS AgEcon
Wilson, William W.; Janzen, Edward L.; Dahl, Bruce L.; Wachenheim, Cheryl J..
Development of genetically modified (GM) wheat varieties is proceeding; however, several critical issues remain the focus of contention. This project summarizes the current state of knowledge on some of these critical issues for commercialization of GM wheats. Background on the evolution of GM Wheats is presented. Then, agronomic adoption and competitiveness of GM crops; research on GM traits in wheat; consumer acceptance of GM crops (a separate section is included on issues related to consumer acceptance of GM crops); regulatory issues and status; international trade; testing, segregation, and identity preservation; and production and marketing risks are examined. Finally, there is a description of the likely marketing system to evolve and a...
Tipo: Working or Discussion Paper Palavras-chave: Wheat; Genetic modification; Transgenic; Marketing; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2003 URL: http://purl.umn.edu/23497
Imagem não selecionada

Imprime registro no formato completo
Transgenic Trees: Implementation and Outcomes of the Plant Protection Act AgEcon
Sedjo, Roger A..
The responsibility for protecting U.S. agriculture from pests and diseases is assigned by the Federal Plant Pest Act (FPPA) to the Animal and Plant Health Inspection Service (APHIS) of the Department of Agriculture. The Plant Protection Act (Title 7 U.S.C. Sections 7701 et seq.) gives Aphis statutory authority over genetically modified organisms (GMO), in effect assigning to APHIS a related responsibility of determining whether a genetically altered plant, crop, or tree is likely to pose unacceptable risks to the environment. Although APHIS has considerable experience with crop plants, it has only limited experience with trees. Yet the possible benefits of applying genetic engineering to trees are substantial and include industrial wood production and...
Tipo: Working or Discussion Paper Palavras-chave: Forestry; Biotechnology; Transgenic; Tree plantations; Timber supply; Genes; GMOs; Industrial wood; Economics; Regulations; Costs; Benefits; Conservation; Resource /Energy Economics and Policy; Q21; Q23; Q16; O32; L73.
Ano: 2004 URL: http://purl.umn.edu/10629
Imagem não selecionada

Imprime registro no formato completo
Valuing Transgenic Cotton Technologies Using a Risk/Return Framework AgEcon
Bryant, Kelly J.; Reeves, Jeanne M.; Nichols, Robert L.; Greene, Jeremy K.; Tingle, Christopher H.; Studebaker, Glenn E.; Bourland, Fred M.; Capps, Charles D.; Groves, Frank E..
Stochastic Efficiency with Respect to a Function (SERF) is used to rank transgenic cotton technology groups and place an upper and lower bound on their value. Yield and production data from replicated plot experiments are used to build cumulative distribution functions of returns for nontransgenic, Roundup Ready, Bollgard, and stacked gene cotton cultivars. Analysis of Arkansas data indicated that the stacked gene and Roundup Ready technologies would be preferred by a large number of risk neutral and risk averse producers as long as the costs of the technology and seed are below the lower bounds calculated in this manuscript.
Tipo: Journal Article Palavras-chave: Cotton; Financial risk; Market value; SERF; Transgenic; Agribusiness; Crop Production/Industries; Risk and Uncertainty; Q12; Q16.
Ano: 2008 URL: http://purl.umn.edu/47257
Imagem não selecionada

Imprime registro no formato completo
Consumers Willingness to Pay to Avoid Transgenic Products AgEcon
Ribeiro, Francis Lee; de Noronha, Jose Ferreira; Noronha, Cassia Maria Silva.
The debate on transgenic, which is embebed in asymmetrical information, has as a focal point, the risks and uncertainties to them associated. Many are the concerns and questions about the risks for the human health and the environment. Considering the interests of the productive sector in the transgenic technology, the matter sets itself in terms of how fast it wants to be settled taking into consideration the incapability of establishing certainties about the damages or benefits within the process. With the recent liberation by the Brazilian government for its production the sector starts showing segmentation. Therefore, this issue reflects in the market itself. The question is: are the consumers willing to buy transgenic? As an attempt to answer this...
Tipo: Conference Paper or Presentation Palavras-chave: Transgenic; Contingent Valuation; Measure of Well-being; Demand and Price Analysis; Food Consumption/Nutrition/Food Safety.
Ano: 2005 URL: http://purl.umn.edu/24221
Imagem não selecionada

Imprime registro no formato completo
Welfare Measures and Mandatory Regulation for Transgenic Food in the European Union: A Theoretical Framework for the Analysis AgEcon
Diez, Maria Del Carmen Fernandez.
This paper presents an analytical framework for studying the impact of mandatory labelling regulation for transgenic food. We compare Genetically Modified (GM) and conventional crop markets and identify gains for food processors prior to mandatory labelling and losses after this measure for the GM market. Nevertheless, food processors could obtain gains for conventional products after market disgregation. Finally, consumers will be worse off both for conventional and GM foods unless qualities other than changes to prices are considered.
Tipo: Conference Paper or Presentation Palavras-chave: Welfare; Mandatory labelling; Transgenic; Genetically modified organism; European Union policy; Agricultural and Food Policy; Q18; K32; D62.
Ano: 2005 URL: http://purl.umn.edu/24472
Imagem não selecionada

Imprime registro no formato completo
Ex-Ante Evaluation of Drought Tolerant Varieties in Eastern and Central Africa AgEcon
Kostandini, Genti; Mills, Bradford F.; Mykerezi, Elton.
Tipo: Conference Paper or Presentation Palavras-chave: Transgenic; Drought Tolerance; Household Benefits; Consumer/Household Economics; Crop Production/Industries.
Ano: 2008 URL: http://purl.umn.edu/49372
Registros recuperados: 26
Primeira ... 12 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional