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Development of transformation vectors for the production of potentially high oleate transgenic oil palm Electron. J. Biotechnol.
Masani,Mat Yunus Abdul; Parveez,Ghulam Kadir Ahmad.
The main target of Malaysian Palm Oil Board (MPOB) genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (UbiPro)-driven bar gene for selection of transformants on herbicide (Basta or Bialaphos), and mesocarp-specific promoter (MSP1) for expression of the transgenes [antisense palmitoyl-ACP-thioesterase (PAT) and sense β-ketoacyl-ACP-synthase II (KASII) and sense Δ9-stearoyl-ACP-desaturase (SAD)] potentially responsible for high oleate content in oil palm...
Tipo: Journal article Palavras-chave: Oleate; Transformation vectors; Transgenic oil palm.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300003
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Expression of Bacillus thuringiensis insecticidal protein gene in transgenic oil palm Electron. J. Biotechnol.
Lee,Mei-Phing; Yeun,Li-Huey; Abdullah,Ruslan.
Oil palm, like all other crops, is susceptible to attack from several insect pests causing significant reduction in productivity. In the past, cry genes from Bacillus thuringiensis have been reported to be effective in conferring resistance towards insect pests in crops such as corn and rice. One of the advantages of these toxin proteins is their specificity towards certain harmful insects. A rapid and efficient method was developed for the transformation and evaluation of CryIA(b) expression in oil palm. A recombinant vector was introduced into immature embryos (IEs) of oil palm via the biolistic method. More than 700 putative transformed IEs from independent transformation events were generated. Transient transformation efficiency of 81-100 % was...
Tipo: Journal article Palavras-chave: CryIA(b) gene; Gene expression; Insect resistance; Oil palm transformation; Rapid detection system; Transgenic oil palm.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200005
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