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	Provedor de dados BJMBR (1) Genet. Mol. Biol. (1) Autor Brofman,P.R.S. (1) Carolina,Romero (1) Correa,A. (1) Dallagiovanna,B. (1) Fernando,Lizcano (1) Goldenberg,S. (1) Senegaglia,A.C. (1) Stimamiglio,M.A. (1) Vargas,Diana (1) Zych,J. (1) Mais... Palavra-chave Trichostatin A (2) 5-Aza-2′-deoxycytidine (1) Adipocyte (1) Adipocyte differentiation (1) Deacetylation (1) Demethylation (1) Epigenetics (1) Histones (1) JMJD2C (1) Mesenchymal stem cells (1) PPARγ (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última The epigenetic modifiers 5-aza-2'-deoxycytidine and trichostatin A influence adipocyte differentiation in human mesenchymal stem cells BJMBR Zych,J.; Stimamiglio,M.A.; Senegaglia,A.C.; Brofman,P.R.S.; Dallagiovanna,B.; Goldenberg,S.; Correa,A.. Epigenetic mechanisms such as DNA methylation and histone modification are important in stem cell differentiation. Methylation is principally associated with transcriptional repression, and histone acetylation is correlated with an active chromatin state. We determined the effects of these epigenetic mechanisms on adipocyte differentiation in mesenchymal stem cells (MSCs) derived from bone marrow (BM-MSCs) and adipose tissue (ADSCs) using the chromatin-modifying agents trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2′-deoxycytidine (5azadC), a demethylating agent. Subconfluent MSC cultures were treated with 5, 50, or 500 nM TSA or with 1, 10, or 100 µM 5azadC for 2 days before the initiation of adipogenesis. The differentiation was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesenchymal stem cells; Trichostatin A; 5-Aza-2′-deoxycytidine; Adipocyte differentiation; Epigenetics. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500405 Regulation of adipogenesis by nucelar receptor PPARγ is modulated by the histone demethylase JMJD2C Genet. Mol. Biol. Fernando,Lizcano; Carolina,Romero; Vargas,Diana. A potential strategy to combat obesity and its associated complications involves modifying gene expression in adipose cells to reduce lipid accumulation. The nuclear receptor Peroxisome Proliferator-activated receptor gamma (PPARγ) is the master regulator of adipose cell differentiation and its functional activation is currently used as a therapeutic approach for Diabetes Mellitus type 2. However, total activation of PPARγ induces undesirable secondary effects that might be set with a partial activation. A group of proteins that produce histone demethylation has been shown to modify the transcriptional activity of nuclear receptors. Here we describe the repressive action of the jumonji domain containing 2C/lysine demethylase 4 C (JMJD2C/KDM4C) on PPARγ... Tipo: Info:eu-repo/semantics/article Palavras-chave: PPARγ; Histones; Demethylation; JMJD2C; Deacetylation; Adipocyte; Trichostatin A. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100004 Registros recuperados: 2 Primeira ... 1 ... Última

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