|
|
|
|
|
| Ribas, Rodolfo Krüger da Câmara; Carboni, Diórgenes dos Santos; Cazarolli, Juciana Clarice; Flôres, Simone Hickmann; Ramirez-Castrillon, Maurício; Valente, Patricia. |
| Lipases (E.C. 3.1.1.3) are serine-hydrolases, and act on long chain fatty acid ester bonds. They exhibit specific and enantioselective activities, which are desirable for many industrial applications. This study aimed at screening and optimizing the production of lipases by wild yeast strains from a variety of substrates, as well as characterizing the enzyme. An initial selection was made in oxygenated oil-supplemented minimum medium, and the enzymatic activity of the supernatant was tested over p- nitrophenyl palmitate. One-hundred and twenty-four yeast strains from different substrates were tested, and twenty-three showed significantly higher lipolytic activity (p <0.01). One yeast in particular, QU110, showed best lipase production and therefore... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: P-NPP; Tryptone; Yeast; Palmitate; Response Surface Model; Serine-hydrolase. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/45481 |
| |
|
|
| Rossi-Rodrigues,Bianca Caroline; Brochetto-Braga,Márcia Regina; Tauk-Tornisielo,Sâmia Maria; Carmona,Eleonora Cano; Arruda,Valeska Marques; Chaud Netto,José. |
| Trichoderma is one of the fungi genera that produce important metabolites for industry. The growth of these organisms is a consequence of the nutritional sources used as also of the physical conditions employed to cultivate them. In this work, the automated Bioscreen C system was used to evaluate the influence of different nutritional sources on the growth of Trichoderma strains (T. hamatum, T. harzianum, T. viride, and T. longibrachiatum) isolated from the soil in the Juréia-Itatins Ecological Station (JIES), São Paulo State - Brazil.The cultures were grown in liquid culture media containing different carbon- (2%; w/v) and nitrogen (1%; w/v) sources at 28ºC, pH 6.5, and agitated at 150 rpm for 72 h. The results showed, as expected, that glucose is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioscreen C system; Growth; Trichoderma; Tryptone; Yeast extract. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200035 |
| |
|
|
|
