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| Del Valle,Paulo Roberto; Milani,Cintia; Brentani,Maria Mitzi; Katayama,Maria Lucia Hirata; Lyra,Eduardo Carneiro de; Carraro,Dirce Maria; Brentani,Helena; Puga,Renato; Lima,Leandro A.; Rozenchan,Patricia Bortman; Nunes,Bárbara dos Santos; Góes,João Carlos Guedes Sampaio; Folgueira,Maria Aparecida Azevedo Koike. |
| Cancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bone marrow; Breast neoplasm; Fibroblast; Gene expression profiling; Lymph node; Mesenchymal stromal cells; Tumor microenvironment. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400002 |
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| Moritz,Milene Nóbrega de Oliveira; Eustáquio,Lívia Mara Santos; Micocci,Kelli Cristina; Nunes,Ana Carolina Caetano; Santos,Patty Karina dos; Vieira,Tamires de Castro; Selistre-de-Araujo,Heloísa Sobreiro. |
| Abstract Background Matrix metalloproteinases (MMPs) are key players in tumor progression, helping tumor cells to modify their microenvironment, which allows cell migration to secondary sites. The role of integrins, adhesion receptors that connect cells to the extracellular matrix, in MMP expression and activity has been previously suggested. However, the mechanisms by which integrins control MMP expression are not completely understood. Particularly, the role of α2β1 integrin, one of the major collagen I receptors, in MMP activity and expression has not been studied. Alternagin-C (ALT-C), a glutamate-cysteine-aspartate-disintegrin from Bothrops alternatus venom, has high affinity for an α2β1 integrin. Herein, we used ALT-C as a α2β1 integrin ligand to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ALT-C; Α2β1; Integrin; Cancer; Tumor microenvironment; MMP; C-Myc. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100307 |
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