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Optimization of Fermentation Medium for Enhanced Glucansucrase and Glucan Production from Weissella confusa BABT
Shukla,Shraddha; Goyal,Arun.
A glucan producing Weissella confusa isolated from fermented cabbage, as identified earlier, was used for optimization of its fermentation medium. The effects of various macronutrients such as sucrose, glucose as cosubstrate, yeast extract, beef extract, peptone, sodium acetate, K2HPO4 and Tween 80 were studied on glucansucrase and glucan production from Weissella confusa. The medium used as control gave 6.0 U/ml enzyme activity and 34 mg/ml glucan concentration. Sucrose (5%), glucose as cosubstrate (5% for glucansucrase and 3% for glucan production), Tween 80 (0.1%), yeast extract (1.5%), Peptone (2.0%) and K2HPO4 (1.5%) were effective nutrients displaying higher glucansucrase and glucan production giving 18.2, 18.0, 7.0, 6.4, 6.2 and 6.4 U/ml enzyme...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Weissella confusa; Glucansucrase; Glucan; Tween 80.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600006
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Enhanced production of manganese peroxidase by Phanerochaete chrysosporium BABT
Urek,Raziye Ozturk; Pazarlioglu,Nurdan Kasikara.
Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 %, v/v) and Mn2+ (174 µM) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phanerochaete chrysosporium; Manganese dependent peroxidase; Tween 80; Mn2+; Phenylmethylsulphonylfloride.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700001
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Spray Drying of Pequi Pulp: Process Performance and Physicochemical and Nutritional Properties of the Powdered Pulp BABT
Santana,Audirene; Kurozawa,Louise; Oliveira,Rafael; Park,Kil.
The objective of this work was to optimize the spray drying of pequi pulp using maltodextrin as carrier agent and Tween 80 as surfactant agent. A central composite rotatable design was used to evaluate the influence of inlet air temperature (140 to 200°C), maltodextrin (15 to 30%) and surfactant (0 to 5%) concentration on the process performance and physicochemical and nutritional properties of the dried powdered pulp. The dependent variables were process yield (27.4 - 51.7%), outlet air temperature (106.5 - 135°C), energetic efficiency (29.9 - 44.8%), moisture content (0.25 - 1.43%), water activity (0.09 to 0.21), hygroscopicity (9.1 - 12.1 g adsorbed moisture/100g dry matter), vitamin C content (129.8 - 303.0 mg/g solids pequi) and total carotenoids...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fruit; Tween 80; Powder property; Screening design; Microscopy.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100500
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