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	Provedor de dados BABT (1) BJM (1) Autor Araújo,Elza Fernandes de (1) Banerjee,Rintu (1) Dutta,Jayati Ray (1) Lima,Juliana Oliveira (1) Pereira,Jorge Fernando (1) Queiroz,Marisa Vieira de (1) Mais... Palavra-chave Ultraviolet mutagenesis (2) 2-Deoxy-d-glucose (1) Extracellular protease (1) Kinetic characterization (1) Pectinolytic complex (1) Physicochemical (1) Pseudomonas sp. (1) Purification (1) Spontaneous mutant (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2017 (1) 2006 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Isolation and characterization of a newly isolated Pseudomonas mutant for protease production BABT Dutta,Jayati Ray; Banerjee,Rintu. A potent bacterium for extracellular protease production was isolated from local soil and identified as Pseudomonas sp. RAJR 044. A mutant of this strain JNGR 242 with protease productivity 2.5 fold higher was obtained by ultraviolet irradiation under experimentally optimized conditions of pH 7.0, temperature of 34ºC, inoculum volume of 1.0 mL and incubation time of 24 hours. Comparative analysis of the chemical characteristics i.e. assimilation of carbon and nitrogen sources were also carried out. Maximum growth of the mutant strain in 2% gelatin agar plate was obtained in presence of dextrose (2%), maltose (2%), ammonium sulfate (2%) and potassium nitrate (2%) whereas, that of the parent strain was found in sucrose (2%) and ammonium nitrate (2%). The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular protease; Purification; Kinetic characterization; Ultraviolet mutagenesis; Pseudomonas sp.; Physicochemical. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100005 Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression BJM Lima,Juliana Oliveira; Pereira,Jorge Fernando; Araújo,Elza Fernandes de; Queiroz,Marisa Vieira de. Abstract Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. Three spontaneous and nine UV-induced mutants were obtained. Some mutants produced sectors (segments morphologically different) that were also studied. The mutants were analyzed for pectinases production on pectinase-agar plates and five mutants and two sectors showing larger clearing zones than the wild type were selected for quantitative assay. Although PL production higher than the wild type has been... Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-Deoxy-d-glucose; Ultraviolet mutagenesis; Pectinolytic complex; Spontaneous mutant. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300602 Registros recuperados: 2 Primeira ... 1 ... Última

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