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Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies Scientia Agricola
Nascimento,Ildon Rodrigues do; Maluf,Wilson Roberto; Figueira,Antônia Reis; Menezes,Cícero Beserra; Resende,Juliano Tadeu Vilela de; Faria,Marcos Ventura; Nogueira,Douglas Willian.
The SCAR (Sequence Characterized Amplified Region) 'Sw-421' molecular marker is located at 1.0 cM from the Sw-5 allele, originated from Lycopersicon peruvianum (L.), which confers resistance to the tomato spotted wilt virus (TSWV). However, it had not been tested yet in advanced tomato populations. The goal of this study was to distinguish resistant homozygotes (Sw-5/Sw-5) and heterozygotes (Sw-5/Sw-5+) from susceptible (Sw-5+/Sw-5+) plants in crossing populations with the Stevens cultivar and advanced backcrossing populations by using 'Sw421' SCAR marker. The amplification of 940 bp and 900 bp bands characterized the resistant homozygotes and susceptible controls, respectively. A two band pattern (900 bp and 940 bp) was observed in heterozygote genotypes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; Sw-5; Vírus resistance; Molecular marker.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300003
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