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| MÜLLER,ILSE; MEDINA-SELBY,ANGELICA; PALACIOS,JOSE LUIS; MARTINEZ,PATRICIO; OPAZO,PATRICIO; BRUCE,ELSA; MANCILLA,MARTA; VALENZUELA,PABLO; YUDELEVICH,ARTURO; VENEGAS,ALEJANDRO. |
| We have cloned and sequenced ten Helicobacter pylori genes from a Chilean strain (CH-CTX1) including: a cytotoxin VacA fragment, a CagA fragment (A17), a species-specific protein (TsaA), urease subunits (UreA, UreB), a flagellin subunit (FlaB), heat shock proteins (HspA and HspB), adhesin (HpaA) and a lipoprotein (Lpp20). We compared their deduced amino acid sequences with the corresponding sequences from three unrelated H. pylori strains, including fully sequenced strains 26695(UK) and J99(USA), and found that eight of them (UreA, UreB, FlaB, HspA, HspB, Lpp20, TsaA and HpaA) presented more than 97.3% identity. In contrast, VacA partial sequence showed lower identity values (93.2 - 94.9%). Moreover, we found major differences in the A17 region respect to... |
| Tipo: Journal article |
Palavras-chave: Chilean H. pylori strain; PCR-cloned genes; HpaA; VacA; CagA sequences. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100010 |
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| Cogo,Laura Lúcia; Monteiro,Cristina Leise Bastos; Nogueira,Keite da Silva; Palmeiro,Jussara Kasuko; Ribeiro,Marcelo Lima; Camargo,Eloá Ramalho de; Neves,Daniel Locatelli; Nascimento,Aguinaldo José do; Costa,Libera Maria Dalla. |
| Prevalence of H. pylori infection was determined using cultures of gastric biopsy samples of patients attended at the academic hospital of the Federal University of Paraná, Curitiba, Paraná, Brazil. Molecular methods were used to characterize the cagA and vacA genes from bacterial isolates associated with different diseases presented by patients. Out of a total of 81, forty-two gastric biopsy samples tested were positive for H. pylori, with a prevalence of 51.9%. No significant difference was found with regard to the gender (p=0.793) and age (p=0.183) of the patients. Genotype s1m1 vacA gene was found in 67% of the cases of peptic ulcer investigated (p=1.0), despite the limited number of patients with this disease (n=3). A correlation between the presence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Virulence genes; CagA; VacA. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400007 |
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| Rasmussen,LT; Labio,RW de; C Neto,A; Silva,LC; Queiroz,VF; Smith,MAC; Payão,SLM. |
| Helicobacter pylori, a gram-negative bacterium, possesses two important virulence factors: the vacuolating toxin (vacA), and the cytotoxin-associated gene product (cagA). The aim of the present study was to evaluate the presence of H. pylori in the stomach and oral cavity of humans and compare the cagA and vacA genotypes of H. pylori found in different samples (stomach, saliva and dental plaque) from the same patient. Gastric biopsies, saliva and dental plaques were obtained from 62 dyspeptic adults. DNA was extracted and evaluated for the presence of H. pylori and the alleles cagA and vacA. Persons with gastritis had a higher frequency of H. pylori -positive samples in the stomach while positive samples from gastric biopsies were significantly correlated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; CagA; VacA; Dental plaque; Dyspeptic patient; Saliva. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200008 |
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