Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Biol. Res. (2) Autor GILLEY,CYNTHIA (1) GÖTTE,MARTIN (1) KASSA,AZEB (1) LINDER,MARIA C (1) MORIYA,MIZUE (1) STADTBÄUMER,ANDREA (1) WHON,ANNE (1) Mais... Palavra-chave Vesicular transport (2) Apotransferrin (1) Caco2 cells (1) Copper (1) Endosomal trafficking (1) Intestinal iron absorption (1) Manganese (1) PEP12 (1) SNARE (1) Syntaxin (1) Vacuole protein sorting (1) Mais... Tipo do documento Journal article (2) Ano 2006 (1) 2002 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae Biol. Res. GÖTTE,MARTIN; STADTBÄUMER,ANDREA. The molecular mechanisms of vesicular protein transport in eukaryotic cells are highly conserved. Members of the syntaxin family play a pivotal role in the membrane fusion process. We have expressed rat syntaxin 6 and its cytoplasmic domain in wild-type and pep12 mutant strains of Saccharomyces cerevisiae to elucidate the role of the syntaxin 6-dependent vesicular trafficking step in yeast. Immunofluorescence microscopy revealed a punctate, Golgi-like staining pattern for syntaxin 6, which only partially overlapped with Pep12p in wild-type yeast cells. In contrast to Pep12p, syntaxin 6 was not mislocalized to the vacuole upon expression from 2 micron vectors, which might be attributed to conserved sorting and retention signals. Syntaxin 6 was not capable... Tipo: Journal article Palavras-chave: Vesicular transport; SNARE; Syntaxin; Endosomal trafficking; Vacuole protein sorting; PEP12. Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300009 Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers Biol. Res. LINDER,MARIA C; MORIYA,MIZUE; WHON,ANNE; KASSA,AZEB; GILLEY,CYNTHIA. Two aspects of the mechanisms by which iron is absorbed by the intestine were studied in the Caco2 cell model, using 59Fe(II)-ascorbate. Data showing the importance of vesicular processes and cycling of apotransferrin (apoTf) to uptake and overall transport of Caco2 cell monolayers (or basolateral 59Fe release) were obtained by comparing effects of: a) adding apoTf to the basal chamber; b) adding vesicular transport inhibitors; or c) cooling to 4ºC. These showed that apoTf may be involved in as much as half of Fe transfer across the basolateral membrane, and that vesicular processes may also play a role in non-apoTf-dependent Fe transport. Studies were initiated to examine potential interactions of other metal ions with Fe(II) via DMT1. Kinetic data showed... Tipo: Journal article Palavras-chave: Intestinal iron absorption; Caco2 cells; Apotransferrin; Vesicular transport; Copper; Manganese. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100016 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco