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| Fakruddin,Md; Rahaman,Mizanur; Ahmed,Monzur Morshed; Hoque,Md Mahfuzul. |
| BACKGROUND: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was to isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh. RESULT: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical characteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp. specific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately high antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates were capable of producing biofilm (strong biofilm producer), extracellular protease and... |
| Tipo: Journal article |
Palavras-chave: Cronobacter; Food; Virulent; Bangladesh. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100057 |
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| Forster,Karine M.; Hartwig,Daiane D.; Seixas,Fabiana K.; McBride,Alan J.A.; Monte,Leonardo G.; Recuero,Ana Lúcia C.; Brod,Claudiomar S.; Hartleben,Cláudia P.; Amaral,Marta; Dellagostin,Odir A.. |
| Pathogenic Leptospira spp. are the etiological agents of leptospirosis, an important disease of both humans and animals. In urban settings, L. interrogans serovars are the predominant cause of disease in humans. The purpose of this study was to characterize a novel Leptospira isolate recovered from an abandoned swimming pool. Molecular characterization through sequencing of the rpoB gene revealed 100% identity with L. interrogans and variable-number tandem-repeat (VNTR) analysis resulted in a banding pattern identical to L. interrogans serogroup Icterohaemorrhagiae, serovar Copenhageni or Icterohaemorrhagiae. The virulence of the strain was determined in a hamster model of lethal leptospirosis. The lethal dose 50% (LD50) was calculated to be two... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Leptospirosis; Virulent; VNTR; RpoB. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100024 |
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