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| Traesel,C.K.; Bernardes,L.M.; Spilki,F.R.; Weiblen,R.; Flores,E.F.. |
| Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) share high genetic and antigenic similarities, but exhibit marked differences in tissue tropism and neurovirulence. The amino-terminal region of glycoprotein C (gC), which is markedly different in each of the viruses, is involved in virus binding to cellular receptors and in interactions with the immune system. This study investigated the genetic and antigenic differences of the 5′ region of the gC (5′ gC) gene (amino-terminal) of South American BoHV-1 (n=19) and BoHV-5 (n=25) isolates. Sequence alignments of 374 nucleotides (104 amino acids) revealed mean similarity levels of 97.3 and 94.2% among BoHV-1 gC (gC1), respectively, 96.8 and 95.6% among BoHV-5 gC (gC5), and 62 and 53.3% between gC1 and gC5.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GC amino-terminal; BoHV; Genetic diversity; Epitope prediction; Virus neutralization. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500470 |
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| Gouveia,Frederico Leite; Oliveira,Renata Mendes Batista de; Lucena,Antônio Edson de Souza; Cordeiro,Marli Tenório; Leite,Ana Cristina Lima. |
| Dengue represents an important health problem in Brazil and therefore there is a great need to develop a vaccine or treatment. The neutralization of the dengue virus by a specific antibody can potentially be applied to therapy. The present paper describes, for the first time, the preparation of Immunoglobulin specific for the dengue virus (anti-DENV IgG), collected from screened Brazilian blood-donations. Production was performed using the classic Cohn-Oncley process with minor modifications. The anti-DENV IgG was biochemically and biophysically characterized and fulfilled the requirements defined by the European Pharmacopoeia. The finished product was able to neutralize different virus serotypes (DENV-1, DENV-2, and DENV-3), while a commercial IgG... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Viral infections; Dengue/treatment; Dengue/virus; Dengue/neutralization; Immunoglobulins/preparation; Plasma fractionation; Virus neutralization. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400022 |
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