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	Provedor de dados 116 (1) 52 (1) Autor Hours,Roque Alberto (2) Martos,María Alicia (2) Butiuk,Ana Paula (1) Combina,Mariana (1) Garro,Oscar Alfredo (1) Rojas,Natalia Lorena (1) Zubreski,Emilce Roxana (1) Mais... Palavra-chave Polygalacturonase (2) Wickerhamomyces anomalus (2) Cassava (1) Characterization (1) Maceration (1) Purification (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2014 (1) 2013 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus 52 Martos,María Alicia; Butiuk,Ana Paula; Rojas,Natalia Lorena; Hours,Roque Alberto. The aim of this work was to study the purification and physicochemical properties of an endo-polygalacturonase (PG) produced by Wickerhamomyces anomalus isolated from the citrus fruit peels. The enzyme was purified to homogeneity from the culture filtrate of W. anomalus grown on the yeast nitrogen base medium with glucose as carbon and energy source and citrus pectin as inductor. After anion-exchange chromatography and gel filtration chromatography, PG activity was eluted as a single peak, yielding 21% of the original activity. After dialysis and cation-exchange chromatography, only one fraction with PG activity was obtained, recovering 56% of initial enzyme activity and 1.3-fold increase in specific activity. The molecular weight of the enzyme was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Wickerhamomyces anomalus; Polygalacturonase; Purification; Characterization. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400587 Isolation of a yeast strain able to produce a polygalacturonase with maceration activity of cassava roots 116 Martos,María Alicia; Zubreski,Emilce Roxana; Combina,Mariana; Garro,Oscar Alfredo; Hours,Roque Alberto. The objective of the present study was the isolation of a yeast strain, from citrus fruit peels, able to produce a polygalacturonase by submerged fermentation with maceration activity of raw cassava roots. Among 160 yeast strains isolated from citrus peels, one strain exhibited the strongest pectinolytic activity. This yeast was identified as Wickerhamomyces anomalus by 5.8S-ITS RFLP analysis and confirmed by amplification of the nucleotide sequence. The yeast produced a polygalacturonase (PG) in Erlenmeyer shake flasks containing YNB, glucose, and citrus pectin. PG synthesis occurred during exponential growth phase, reaching 51 UE.mL-1 after 8 hours of fermentation. A growth yield (Yx/s) of 0.43 gram of cell dry weight per gram of glucose consumed was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Wickerhamomyces anomalus; Polygalacturonase; Maceration; Cassava. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000200018 Registros recuperados: 2 Primeira ... 1 ... Última

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