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Registros recuperados: 7
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Detection and characterization of Wolbachia infection in silkworm Genet. Mol. Biol.
Zha,Xingfu; Zhang,Wenji; Zhou,Chunyan; Zhang,Liying; Xiang,Zhonghuai; Xia,Qingyou.
Wolbachia naturally infects a wide variety of arthropods, where it plays important roles in host reproduction. It was previously reported that Wolbachia did not infect silkworm. By means of PCR and sequencing we found in this study that Wolbachia is indeed present in silkworm. Phylogenetic analysis indicates that Wolbachia infection in silkworm may have occurred via transfer from parasitic wasps. Furthermore, Southern blotting results suggest a lateral transfer of the wsp gene into the genomes of some wild silkworms. By antibiotic treatments, we found that tetracycline and ciprofloxacin can eliminate Wolbachia in the silkworm and Wolbachia is important to ovary development of silkworm. These results provide clues towards a more comprehensive understanding...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wolbachia; Silkworm; Wsp; Antibiotics.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400014
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First record of Wolbachia in South American terrestrial isopods: prevalence and diversity in two species of Balloniscus (Crustacea, Oniscidea) Genet. Mol. Biol.
Almerão,Mauricio Pereira; Fagundes,Nelson Jurandi Rosa; Araújo,Paula Beatriz de; Verne,Sébastien; Grandjean,Frédéric; Bouchon,Didier; Araújo,Aldo Mellender.
Wolbachia are endosymbiotic bacteria that commonly infect arthropods, inducing certain phenotypes in their hosts. So far, no endemic South American species of terrestrial isopods have been investigated for Wolbachia infection. In this work, populations from two species of Balloniscus (B. sellowii and B. glaber) were studied through a diagnostic PCR assay. Fifteen new Wolbachia 16S rDNA sequences were detected. Wolbachia found in both species were generally specific to one population, and five populations hosted two different Wolbachia 16S rDNA sequences. Prevalence was higher in B. glaber than in B. sellowii, but uninfected populations could be found in both species. Wolbachia strains from B. sellowii had a higher genetic variation than those isolated from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wolbachia; Prevalence; Diversity; South America; Oniscidea.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600013
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Padronização das análises moleculares para a pesquisa de Wolbachia em artrópodes parasitas de interesse pecuário. Infoteca-e
CHAPAVAL, L.; BRITO, L. G.; BARBIERI, F. da S.; MALAGO JUNIOR, W.; BILHASSI, T. B..
Insetos e outros artrópodes são hospedeiros comuns de bactérias transmitidas por via materna (Duron; Hurst, 2013). Esses endossimbiontes hereditários podem ter uma forte influência na ecologia do hospedeiro. Tais bactérias transmitidas verticalmente são numerosas e dependentes do hospedeiro para sobrevivência e transmissão. Certas associações são obrigatórias para ambos os parceiros, e geralmente envolvem um benefício nutricional para o hospedeiro. Outras bactérias hereditárias são facultativas e variam, em associações, de mutualistas a parasitas quando observadas sob a perspectiva do hospedeiro. Entre estas, a Wolbachia é a mais comum e a mais difundida simbionte facultativa de insetos e artrópodes (Hilgenboecker et al., 2008; Zug; Hammerstein, 2012).
Tipo: Circular Técnica (INFOTECA-E) Palavras-chave: Endossimbionte hereditário; Ecologia do hospedeiro; Wolbachia.
Ano: 2020 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1120107
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The Cytoplasm Affects the Epigenome in Drosophila melanogaster ArchiMer
Grunau, Christoph; Voigt, Susanne; Dobler, Ralph; Dowling, Damian K.; Reinhardt, Klaus.
Cytoplasmic components and their interactions with the nuclear genome may mediate patterns of phenotypic expression to form a joint inheritance system. However, proximate mechanisms underpinning these interactions remain elusive. To independently assess nuclear genetic and epigenetic cytoplasmic effects, we created a full-factorial design in which representative cytoplasms and nuclear backgrounds from each of two geographically disjunct populations of Drosophila melanogaster were matched together in all four possible combinations. To capture slowly-accumulating epimutations in addition to immediately occurring ones, these constructed populations were examined one year later. We found the K4 methylation of histone H3, H3K4me3, an epigenetic marker...
Tipo: Text Palavras-chave: Epigenetics; Mitochondria; Mito-nuclear coevolution; Wolbachia.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00478/58935/62427.pdf
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Wolbachia, una pandemia con posibilidades Rev. Soc. Entomol. Argent.
Rodriguero,Marcela S..
La infección causada por Wolbachia es la más extendida entre los animales. La capacidad de esta bacteria para manipular la reproducción de sus hospedadores la posicionan en el centro de la biología de los organismos, influyendo en procesos tan capitales como la determinación del sexo, el ciclo celular, la formación y extinción de especies y el comportamiento de artrópodos entre los que se cuentan varias plagas y vectores de enfermedades. Cualidades tales como la herencia vertical de Wolbachia, la velocidad a la que se propaga en las poblaciones que afecta, la capacidad de bloquear la actividad patogénica de diversos microorganismos o de acortar el ciclo de vida de sus hospedadores la señalan como un potencial instrumento para el control de poblaciones de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wolbachia; Pandemia; Transferencia horizontal; Técnica del insecto incompatible; Incompatibilidad citoplasmática; Partenogénesis telitóquica; Feminización; Androcidio; Parásito reproductivo.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802013000200001
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The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps Anais da ABC (AABC)
MARTINELLI,ARTURO B.; WAICHERT,CECILIA; BARBOSA,DIEGO N.; FAGUNDES,VALÉRIA; AZEVEDO,CELSO O..
ABSTRACT Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera: Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diaphanization; Genitalia preparation; KOH; Wolbachia.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401629
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Detection of Wolbachia (Alphaproteobacteria: rickettsiales) in three species of terrestrial isopods (crustacea: isopoda: oniscidea) in Brazil BJM
Zimmermann,Bianca Laís; Almerão,Maurício Pereira; Bouchon,Didier; Araujo,Paula Beatriz.
Terrestrial isopods are widely infected with Wolbachia. However, little is known about the presence of bacteria in the Neotropical species. The objective of this study was to test the hypothesis of presence of Wolbachia infection in the native species of terrestrial isopods, Atlantoscia floridana and Circoniscus bezzii, and in the introduced species Burmoniscus meeusei.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wolbachia; Terrestrial isopods; 16S rDNA; Brazil.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200036
Registros recuperados: 7
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