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| NIKLITSCHEK,MAURICIO; ALCAÍNO,JENNIFER; BARAHONA,SALVADOR; SEPÚLVEDA,DIONISIA; LOZANO,CARLA; CARMONA,MARISELA; MARCOLETA,ANDRÉS; MARTÍNEZ,CLAUDIO; LODATO,PATRICIA; BAEZA,MARCELO; CIFUENTES,VÍCTOR. |
| The cloning and nucleotide sequence of the genes (idi, crtE, crtYB, crtl and crtS) controlling the astaxanthin biosynthesis pathway of the wild-type ATCC 24230 strain of Xanthophyllomyces dendrorhous in their genomic and cDNA version were obtained. The idi, crtE, crtYB, crtl and crtS genes were cloned, as fragments of 10.9, 11.5, 15.8, 5.9 and 4 kb respectively. The nucleotide sequence data analysis indicates that the idi, crtE, crtYB, crtl and crtS genes have 4, 8,4, 11, and 17 introns and 5, 9, 5, 12 and 18 exons respectively. In addition, a highly efficient site-directed mutagenesis system was developed by transformation by integration, followed by mitotic recombination (the double recombinant method). Heterozygote idi (idi+ / idi-::hph), crtE (crtE+ /... |
| Tipo: Journal article |
Palavras-chave: Astaxanthin biosynthesis; Xanthophyllomyces dendrorhous; Phaffia rhodozyma. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100011 |
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| LODATO,PATRICIA; ALCAÍNO,JENNIFER; BARAHONA,SALVADOR; RETAMALES,PATRICIO; JIMÉNEZ,ANTONIO; CIFUENTES,VÍCTOR. |
| The expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to correlate astaxanthin production with the transcript levels of carotenogenic genes in a wild-type strain (UCD 67-385) and two overproducer deregulated strains (atxS1 and atxS2). At 3 day cultures, the wild-type strain contained higher transcript levels from the crtE and crtYB genes on minimal medium than on rich medium. Similarly, carotenoid production was higher on minimal medium than on rich medium. However, carotenoid production in the atxS1 and atxS2 strains was... |
| Tipo: Journal article |
Palavras-chave: Carotenoid biosynthesis; Overproducer strains; Xanthophyllomyces dendrorhous; Phaffia rhodozyma. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100009 |
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| LODATO,P; ALCAÍNO,J; BARAHONA,S; NIKLITSCHEK,M; CARMONA,M; WOZNIAK,A; BAEZA,M; JIMÉNEZ,A; CIFUENTES,V. |
| In the yeast Xanthophyllomyces dendrorhous the genes idi, crtE, crtYB, crtl and ast are involved in the biosynthesis of astaxanthin from isopentenyl pyrophosphate. The carotenoid production and the kinetics of mRNA expression of structural genes controlling the carotenogenesis in a wild-type ATCC 24230 and in carotenoid overproducer deregulated atxS2 strains were studied. The biosynthesis of carotenoid was induced at the late exponential growth phase in both strains. However, the cellular carotenoid concentration was four times higher in atxS2 than in the wild-type strain in the exponential growth phase, suggesting that carotenogenesis was deregulated in atxS2 at the beginning of growth. In addition, the maximum expression of the carotenogenesis genes at... |
| Tipo: Journal article |
Palavras-chave: Xanthophyllomyces dendrorhous; Carotenogenesis genes; MRNA expression. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100008 |
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