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Registros recuperados: 7
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Structure and properties of fibres from sea-grass (Zostera marina) ArchiMer
Davies, Peter; Morvan, C; Sire, O; Baley, C.
This paper presents results from a study of fibres extracted from Zostera marina eel-grass collected from the Baltic coast. This species of sea-grass is shown to contain small diameter (around 5 mu m) fibres composed of similar to 57% cellulose, similar to 38% of non-cellulosic polysaccharides (mainly xylan) and similar to 5% of residual matter so-called Klason lignin. This composition is quite different to that of commonly used terrestrial fibres. Single fibre stiffness values up to 28 GPa were measured. This stiffness combined with a low density could provide an attractive reinforcement for composite materials, and may be particularly suitable for use in bio-degradable structures.
Tipo: Text Palavras-chave: Xylan; Tension; Mechanical behaviour; IR spectroscopy; Sea grass; Fibre.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2607.pdf
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Purification and characterization of a low molecular weight xylanase from solid-state cultures of Aspergillus fumigatus Fresenius Rev. Microbiol.
Silva,Claudio Henrique Cerri e; Puls,Jurgen; Sousa,Marcelo Valle de; Ferreira Filho,Edivaldo Ximenes.
A xylan-degrading enzyme (xylanase II) was purified to apparent homogeneity from solid-state cultures of Aspergillus fumigatus Fresenius. The molecular weight of xylanase II was found to be 19 and 8.5 kDa, as estimated by SDS-PAGE and gel filtration on FPLC, respectively. The purified enzyme was most active at 55 °C and pH 5.5. It was specific to xylan. The apparent Km and Vmax values on soluble and insoluble xylans from oat spelt and birchwood showed that xylanase II was most active on soluble birchwood xylan. Studies on hydrolysis products of various xylans and xylooligomers by xylanase II on HPLC showed that the enzyme released a range of products from xylobiose to xylohexaose, with a small amount of xylose from xylooligomers, and presented transferase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylan; Xylanase; Aspergillus fumigatus.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200005
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Application of xylanases from Amazon Forest fungal species in bleaching of eucalyptus kraft pulps BABT
Medeiros,Roseli Garcia; Silva Jr.,Francides Gomes da; Báo,Sônia Nair; Hanada,Rogério; Ferreira Filho,Edivaldo Ximenes.
Crude xylanase preparations from Penicillium corylophilum, Aspergillus niger and Trichoderma longibrachiatum were used to treat Eucalyptus kraft pulp, prior to chlorine dioxide and alkaline bleaching sequences. The enzyme pretreatment improved brightness and delignification of non-delignified and oxygen-bleached samples of eucalyptus kraft pulp. Xylanase preparations from T. longibrachiatum and P. corylophilum were more effective to reduce pulp kappa number. A small reduction in viscosity was obtained when the oxygen-bleached pulp was treated with xylanase preparation from A. niger. For all enzyme samples, the best release of chromophoric material from the pulp was at 237 nm. The enzyme preparation from P. corylophilum was responsible for the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylan; Xylanase; Kraft pulp; Pulp bleaching.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200008
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Investigation of factors affecting xylanase activity from Trichoderma harzianum 1073 D3 BABT
Isil,Seyis; Nilufer,Aksoz.
In this study, some physiological conditions affecting the activity of xylanase enzyme produced from Trichoderma harzianum 1073 D3 were determined. In addition, stabilization of pH and temperature in liquid and semi-solid state cultivation media were investigated. It was concluded that for maximum xylanase activity, incubation at 60°C in an enzyme incubation medium with pH 5 that contained 1 % xylan was appropriate. The stability studies showed that the enzyme was relatively stable in the pH range 3-7 and retained more than 50 % of its original activity after four months.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Xylan; Xylanase; Lignocellulosic material; Enzyme stability; Metal ions.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200004
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Hydrolysis of xylans by enzyme systems from solid cultures of Trichoderma harzianum strains BJMBR
Silveira,F.Q.P.; Ximenes,F.A.; Cacais,A.O.G.; Milagres,A.M.F.; Medeiros,C.L.; Puls,J.; Filho,E.X.F..
Xylanase activity was isolated from crude extracts of Trichoderma harzianum strains C and 4 grown at 28oC in a solid medium containing wheat bran as the carbon source. Enzyme activity was demonstrable in the permeate after ultrafiltration of the crude extracts using an Amicon system. The hydrolysis patterns of different xylans and paper pulps by xylanase activity ranged from xylose, xylobiose and xylotriose to higher xylooligosaccharides. A purified ß-xylosidase from the Trichoderma harzianum strain released xylose, xylobiose and xylotriose from seaweed, deacetylated, oat spelt and birchwood xylans. The purified enzyme was not active against acetylated xylan and catalyzed the hydrolysis of xylooligosaccharides, including xylotriose, xylotetraose and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Trichoderma; Xylan; Xylanase.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800003
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Purification and characterization of a new Xylanase from Humicola grisea var. Thermoidea BJM
Lucena-Neto,Severino de Albuquerque; Ferreira-Filho,Edivaldo Ximenes.
The thermophilic fungus Humicola grisea var. thermoidea secretes extracellular xylanase when grown on solid and in liquid media containing wheat bran and banana plant residue as substrates, respectively. At 55ºC, xylanase from the culture filtrate of H. grisea var. thermoidea grown on banana stalk retained 50% of its activity after 28 h of incubation. A xylanase (X2) was isolated from solid state cultures with wheat bran as the carbon source. It was purified to apparent homogeneity by ultrafiltration followed by ion-exchange and hydrophobic interaction chromatography on DEAE-Sepharose and Phenyl-Sepharose resins, respectively. The enzyme had an apparent molecular weight of 29 kDa, as determined by SDS-PAGE. The purified enzyme was most active at pH and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Humicola grisea; Xylan; Xylanase.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100014
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Production of xylan-degrading enzymes by a Trichoderma harzianum strain BJM
Cacais,André O.Guerreiro; Silveira,Fabiane Q. de Paula; Ferreira Filho,Edivaldo Ximenes.
Trichoderma harzianum strain 4 produced extracellular xylan-degrading enzymes, namely beta-xylanase, beta-xylosidase and alpha-arabinofuranosidase, when grown in liquid medium cultures containing oat spelt xylan as inducer. Cellulase activity was not detected. The pattern of xylan-degrading enzymes induction was influenced by the form of xylan present in the medium. They were detected in different incubation periods. Electrophoretic separation of the proteins from liquid culture filtrates by SDS-PAGE showed a variety of bands with high and low molecular weights.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Xylan; T. harzianum; Xylanolytic activity.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200014
Registros recuperados: 7
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