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Última Application of xylanases from Amazon Forest fungal species in bleaching of eucalyptus kraft pulps BABT Medeiros,Roseli Garcia; Silva Jr.,Francides Gomes da; Báo,Sônia Nair; Hanada,Rogério; Ferreira Filho,Edivaldo Ximenes. Crude xylanase preparations from Penicillium corylophilum, Aspergillus niger and Trichoderma longibrachiatum were used to treat Eucalyptus kraft pulp, prior to chlorine dioxide and alkaline bleaching sequences. The enzyme pretreatment improved brightness and delignification of non-delignified and oxygen-bleached samples of eucalyptus kraft pulp. Xylanase preparations from T. longibrachiatum and P. corylophilum were more effective to reduce pulp kappa number. A small reduction in viscosity was obtained when the oxygen-bleached pulp was treated with xylanase preparation from A. niger. For all enzyme samples, the best release of chromophoric material from the pulp was at 237 nm. The enzyme preparation from P. corylophilum was responsible for the highest... Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylan; Xylanase; Kraft pulp; Pulp bleaching. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200008 Screening and Molecular Identification of New Microbial Strains for Production of Enzymes of Biotechnological Interest BABT Ghazala,Imen; Haddar,Anissa; Romdhane,Molka Ben; Ellouz-Chaanouni,Semia. ABSTRACT: This research focused on isolation, identification and characterization of new strains of fungi and bacteria, which were able to produce extracellular xylanase, mannanase, pectinase and α-amylase. Fungi isolates were identified on the basis of analyses of 18S gene sequencing and internal transcribed spacer region. The closest phylogenetic neighbors according to 18S gene sequence and ITS region data for the two isolates M1 and SE were Aspergillus fumigatus and Aspergillus sydowii, respectively. I4 was identified as Bacillus mojavensis on the basis of the 16S rRNA gene sequencing and biochemical properties. The enzyme production was evaluated by cultivating the isolated microorganisms in liquid-state bioprocess using wheat bran as carbon source.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylanase; Pectinase; Β-mannanase; Α-amylase; Bacillus mojavensis; Aspergillus fumigatus and Aspergillus sydowii. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100510 Enzymatic programs for broilers BABT Sorbara,José Otávio Berti; Murakami,Alice Eiko; Nakage,Eliane Saiuri; Piracés,Francisco; Potença,Alexandra; Guerra,Rafael Lachinski Holanda. Two trials were conducted on floor pens to evaluate the performance of broilers fed with diets contained different carbohydrases enzyme programs at different ages. Trial 1- The body weight gain (BWG) was affected by the Negative Control (NC) treatment (P<0.05). However, when the birds were fed with the NC diet + Amilase (AMY)+Xilanase (XYL), the BWG improved and reached the same level as the Positive Control (PC). Trial 2- The birds received enzymes supplementation in the total phase and others only in the grower phase.The ME reduction by 120 kcal/kg increased the feed conversion ratio (FCR) (P<0.05) when compared to PC and none of the enzyme programs was able to recover the performance. It can be concluded that it is possible to supplement with AMY... Tipo: Info:eu-repo/semantics/article Palavras-chave: Broilers; Carbohydrase; Enzymes; Amylase; Xylanase. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700030 Influence of agitation speeds and aeration rates on the Xylanase activity of Aspergillus niger SS7 BABT Bakri,Yasser; Mekaeel,Alaa; Koreih,Amal. In this study, the effect of agitation and aeration rates on xylanase activity of Aspergillus niger SS7 in 3-litre stirred tank bioreactor was investigated. The agitation rates tested were 100, 200 and 300 rpm at each airflow rates of 0.5, 1.0 and 1.5 vvm. The maximum xylanase activity in mono- agitator system was at the agitation speed of 200 rpm and aeration rate of 1.0 vvm. In bi-agitator system, at low agitation speed (100 rpm), the xylanase activity was enhanced by 13% compared to mono- agitator system for an aeration rate of 1.0 vvm. Xylanase productivity in continuous culture was higher by approximately 3.5 times than in batch culture. Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Agitation; Aeration; Xylanase. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400003 Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile BABT Joshi,Chetna; Khare,Sunil Kumar. Regulation of xylanase production in two thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile was investigated. The expression of xylanase was found to be inducible in both the cases. Various carbon sources were tested so as to identify the inducers. Soy flour and oat spelt xylan induced maximum level of xylanase in Scytalidium thermophilum and Sporotrichum thermophile respectively. Induction of xylanase in Scytalidium thermophilum led to simultaneous induction of cellulase. The zymography of enzyme preparations revealed that different carbon sources caused differential expression of multiple isoforms of xylanase in Scytalidium thermophilum, but same isoforms were expressed by Sporotrichum thermophile irrespective of the carbon source... Tipo: Info:eu-repo/semantics/article Palavras-chave: Induction; Isoforms; Xylanase; Biobleaching. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100003 Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues BABT Alberton,Luiz Romulo; Vandenberghe,Luciana Porto de Souza; Assmann,Ricardo; Fendrich,Ricardo Cancio; Rodriguéz-León,José; Soccol,Carlos Ricardo. The study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source, respectively. In SmF, Napier grass (1% v/w) supplemented with soybean bran, hydroxyethylcellulose and B complex vitamins were used. Soybean bran (1.5 % w/v), B complex vitamins (0.1%), and hydroxyethilcellulose (0.15%) led to an increase in xylanase production (23.41 U/mL). In SSF, the effects of the following parameters were studied: substrate composition (sugarcane bagasse, Napier grass and soybean bran), initial moisture, and inoculum rate. In SSF, the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; Xylanase; Submerged fermentation; Solid-state agro-industrial fermentation; Residues. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700022 Investigation of factors affecting xylanase activity from Trichoderma harzianum 1073 D3 BABT Isil,Seyis; Nilufer,Aksoz. In this study, some physiological conditions affecting the activity of xylanase enzyme produced from Trichoderma harzianum 1073 D3 were determined. In addition, stabilization of pH and temperature in liquid and semi-solid state cultivation media were investigated. It was concluded that for maximum xylanase activity, incubation at 60°C in an enzyme incubation medium with pH 5 that contained 1 % xylan was appropriate. The stability studies showed that the enzyme was relatively stable in the pH range 3-7 and retained more than 50 % of its original activity after four months. Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Xylan; Xylanase; Lignocellulosic material; Enzyme stability; Metal ions. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200004 Coexpression of rumen fungal xylanase and bifunctional cellulase genes in Escherichia coli BABT Comlekcioglu,Ugur; Gunes,Merva; Altun,Hanifi; Ekiz,Dilek Ozgun; Aygan,Ashabil. ABSTRACT Rumen fungi inhabit the gastro-intestinal tract of ruminants and the most non-ruminant herbivores. Rumen fungi produce highly active plant cell wall degrading enzymes, therefore they have gained scientific interest. In this study, genes encoding xylanase (xynA-7) and cellulase (celA-5) were amplified from Neocallimastix sp. GMLF7 and Orpinomyces sp. GMLF5, respectively, and expressed in Escherichia coli. XynA-7 was found to be active only on xylan, however CelA-5 had activity both on carboxymethyl cellulose and lichenan. Lichenase activity of CelA-5 was found to be higher than carboxymethyl cellulase activity. The optimal conditions were at pH 6.0 and 40 °C for CelA-5 and at pH 6.5 and 50 °C for XynA-7. A coexpression vector was constructed to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rumen fungi; Xylanase; Cellulase; Coexpression; E. coli. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100407 Production and properties of Xylanase from thermophilic Bacillus sp. BABT Cordeiro,Carlos Alberto Martins; Martins,Meire Lelis Leal; Luciano,Angélica Bárbara; Silva,Roberta Freitas da. An aerobic, thermophilic, xylanolytic bacterium was isolated from local soil. The results of 16S rRNA sequence comparisons indicated that the isolate was closely related to Bacillus caldoxylolyticus and Bacillus sp strain AK1. These organisms exhibited 94% levels of ribossomal DNA sequence homology. Studies on the xylanase characterisation from liquid cultures grown on beechwood xylan revealed that the enzyme retained 100% of activity for 2 hours at temperatures ranging from 30 to 50º C, while at 60, 70 and 100º C, 10%, 11% and 29% of the original activities were lost, respectively. The optimum pH of the enzyme was found to be between 6.5 and 7.0. After incubation of crude enzyme solution for 24 hours at 25º C and at pH 5.5 to 8.0, a decrease of about 12%... Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylanase; Thermophilic bacterium; Bacillus sp. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600002 Hydrolysis of xylans by enzyme systems from solid cultures of Trichoderma harzianum strains BJMBR Silveira,F.Q.P.; Ximenes,F.A.; Cacais,A.O.G.; Milagres,A.M.F.; Medeiros,C.L.; Puls,J.; Filho,E.X.F.. Xylanase activity was isolated from crude extracts of Trichoderma harzianum strains C and 4 grown at 28oC in a solid medium containing wheat bran as the carbon source. Enzyme activity was demonstrable in the permeate after ultrafiltration of the crude extracts using an Amicon system. The hydrolysis patterns of different xylans and paper pulps by xylanase activity ranged from xylose, xylobiose and xylotriose to higher xylooligosaccharides. A purified ß-xylosidase from the Trichoderma harzianum strain released xylose, xylobiose and xylotriose from seaweed, deacetylated, oat spelt and birchwood xylans. The purified enzyme was not active against acetylated xylan and catalyzed the hydrolysis of xylooligosaccharides, including xylotriose, xylotetraose and... Tipo: Info:eu-repo/semantics/other Palavras-chave: Trichoderma; Xylan; Xylanase. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800003 Production and characterization of an enzyme complex from a new strain of Clostridium thermocellum with emphasis on its xylanase activity BJM Vieira,Werner Bessa; Moreira,Leonora Rios de Souza; Monteiro Neto,Amadeu; Ferreira Filho,Edivaldo Ximenes. A new bacterial strain (ISO II) was isolated from manure cow and identified as phylogenetically close to the thermophilic cellulolytic bacterium Clostridium thermocellum. The new strain produced extracellular xylanase, pectinase, mannanase and cellulase activities when grown in liquid culture medium containing banana stem as carbon source. The enzyme production profile after growth on banana stem showed that xylanase and cellulase activities were detected in different incubation periods. An enzyme complex containing xylanase, cellulase and mannanase activities was isolated from culture supernatant samples of strainISO II. The complex was partially purified by ultrafiltration and gel filtration chromatography on Sephacryl S-300. Zymogram analysis after... Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium thermocellum; Banana stem; Xylanase. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200009 One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash BJM Sanghi,Ashwani; Garg,Neelam; Gupta,V.K.; Mittal,Ashwani; Kuhad,R.C.. The present study describes the one-step purification and characterization of an extracellular cellulase-free xylanase from a newly isolated alkalophilic and moderately thermophilic strain of Bacillus subtilis ASH. Xylanase was purified to homogeneity by 10.5-fold with ~43% recovery using ion-exchange chromatography through CM-Sephadex C-50. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular mass of 23 kDa. It showed an optimum pH at 7.0 and was stable over the pH range 6.0-9.0. The optimum temperature for enzyme activity was 55 ºC. The purified xylanase did not lose any activity up to 45 ºC, however, it retained 80% and 51% of its activity after pre-incubation at 55 ºC and 60 ºC, respectively. The enzyme obeyed Michaelis-Menton... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkalophilic; Bacillus subtilis; Purification; Xylanase. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200029 Improvement of xylanase production by Cochliobolus sativus in solid state fermentation BJM Bakri,Yasser; Jawhar,Mohammed; Arabi,Mohammed Imad Eddin. The xylanase production by Cochliobolus sativus strain Cs6 was improved under solid state fermentation (SSF). High xylanase activity (1079 U/g) was obtained when wheat straw was used after 8 days of incubation. Combinations of sodium nitrate with peptone or yeast extract resulted in an increased xylanase production (1543 and 1483 U/g, respectively). The Cs6 strain grown in SSF in a simple medium, proved to be a promising microorganism for xylanase production. Tipo: Info:eu-repo/semantics/article Palavras-chave: Cochliobolus sativus; Xylanase; Solid-state fermentation. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300036 Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus miehe BJM Silva,Roberto da; Lago,Ellen S.; Merheb,Carolina W.; Macchione,Mariana M.; Park,Yong Kun; Gomes,Eleni. The use of waste as raw material is important for government economy and natural balance. The purpose of this work was to study the production of CMCase and xylanase by a Brazilian strain of Thermoascus aurantiacus in solid state fermentation (SSF) using different agricultural residues (wheat bran, sugarcane bagasse, orange bagasse, corncob, green grass, dried grass, sawdust and corn straw) as substrates without enrichment of the medium and characterize the crude enzymes.The study of the extracellular cellulolytic and hemicellulolytic enzymes showed that T. arantiacus is more xylanolytic than cellulolytic. The highest levels of enzymes were produced in corncob, grasses and corn straw. All the enzymes were stable at room temperature by 24 h over a broad pH... Tipo: Info:eu-repo/semantics/article Palavras-chave: Thermoascus aurantiacus; Xylanase; CMCase; Solid state fermentation; Agricultural residues. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300006 Cloning of a novel xylanase gene from a newly isolated Fusarium sp. Q7-31 and its expression in Escherichia coli BJM Xie,Zhan-Ling; Gao,Hai-Yan; Zhang,Qian; Wang,Huan; Liu,Ying. A strain of Q7-31 was isolated from Qinghai-Tibet Plateau and was identified as Fusarium sp. based on its morphological characteristics and ITS rDNA gene sequence analysis. It has the highest capacity of degrading cell wall activity compared with other 11 strains. To do research on its xylanase activity of Fusarium sp. Q7-31 while the degrading the rice cell walls, the complete gene xyn8 that encodes endo-1, 4-β-xylanase secreted by Fusarium sp. Q7-31 was cloned and sequenced. The coding region of the gene is separated by two introns of 56bp and 55bp. It encodes 230 amino acid residues of a protein with a calculated molecular weight of 25.7 kDa. The animo acids sequence of xyn8 gene has higher similarity with those of family 11 of glycosyl hydrolases... Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylanase; Gene cloning; Fusariumsp. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100049 Production of cellulase-free xylanase by Trichoderma reesei SAF3 BJM Kar,Sanjay; Mandal,Asish; Mohapatra,Pradeep K. das; Mondal,Keshab C.; Pati,Bikash R.. A xylanase producing fungi has been isolated from soil and identified as Trichoderma reesei SAF3. Maximum growth of the organism was found at 48 h under submerged condition in xylan containing enriched medium, whereas highest enzyme production (4.75U/mL) was recorded at 72 h. No detectable cellulase activity was noted during whole cultivation period. The partially purified enzyme hydrolyzed xylan into xylopentose and xylose. All these properties of xylanase highlighten its promising uses in industrial scale. Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylanase; Trichoderma reesei; Submerged fermentation. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400011 Xylanase and cellulase activities during anaerobic decomposition of three aquatic acrophytes BJM Nunes,Maíra F; Cunha-Santino,Marcela B. da; Bianchini Jr,Irineu. Enzymatic activity during decomposition is extremely important to hydrolyze molecules that are assimilated by microorganisms. During aquatic macrophytes decomposition, enzymes act mainly in the breakdown of lignocellulolytic matrix fibers (i.e. cellulose, hemicellulose and lignin) that encompass the refractory fraction from organic matter. Considering the importance of enzymatic activities role in decomposition processes, this study aimed to describe the temporal changes of xylanase and cellulose activities during anaerobic decomposition of Ricciocarpus natans (freely-floating), Oxycaryum cubense (emergent) and Cabomba furcata (submersed). The aquatic macrophytes were collected in Óleo Lagoon, Luiz Antonio, São Paulo, Brazil and bioassays were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaerobic decomposition; Aquatic macrophytes; Xylanase; Cellulase. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100010 Thermostability of xylanolytic enzymes produced by Lentinula edodes UFV70 BJM Ribeiro,Liliane Fraga Costa; Vaz,Marcelo Gomes Marçal Vieira; Chaves-Alves,Virgínia Maria; Vanetti,Maria Cristina Dantas; Kasuya,Maria Catarina Megumi; Passos,Flávia Maria Lopes; Nascimento,Antônio Galvão do. Xylanolytic enzymes produced by Lentinula edodes UFV70, cultivated in eucalyptus sawdust/rice bran medium, were stable at 50, 60 and 65ºC for 21 hours, losing only 15-25% activity. Fungus incubation at 50ºC for 12 hours and at 65ºC for 24 hours increased the amount of xylose produced. Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylanase; Thermostability; Lentinula edodes; Xylose. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100021 Screening of filamentous fungi for production of enzymes of biotechnological interest BJM Guimarães,Luis Henrique S.; Peixoto-Nogueira,Simone C.; Michelin,Michele; Rizzatti,Ana Carolina S.; Sandrim,Valéria C.; Zanoelo,Fabiana F; Aquino,Ana Carla M.M.; Junior,Altino B.; Polizeli,Maria de Lourdes T. M.. Many enzymes produced by fungi have relevant biotechnological applications in several industrial areas. The purpose of this study was to collect and isolate filamentous fungi from soil and humus, plants and sugar cane bagasse of different regions of the São Paulo state. Forty isolates were examined for their ability to produce xylanase, glucose-oxidase, alkaline phosphatase, acid phosphatase, phytase, pectinase and amylase. Among these, twenty three isolates exhibited enzymatic potential. The xylanases produced by two of these isolates (Aspergillus caespitosus and A. phoenicis) showed good potential for pulp bleaching. Among seventeen isolates, at least three produced high levels of glucose-oxidase, being Rhizopus stolonifer and A. versicolor the best... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphatase; Xylanase; Glucose oxidase; Pectinase; Amylase; Rhizopus microsporus var. rhizopodiformis; Fungi. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400014 Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation BJM Moretti,Marcia M. S.; Bocchini-Martins,Daniela A.; Silva,Roberto Da; Rodrigues,André; Sette,Lara D.; Gomes,Eleni. Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45ºC on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Thermophilic fungi; Solid-state fermentation; Xylanase; Cellulase. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300032 Registros recuperados: 41 Primeira ... 1 2 3 ... Última

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