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	Provedor de dados BJM (1) Genet. Mol. Biol. (1) Autor Almeida,Alzira Maria Paiva de (2) Leal,Nilma Cintra (2) Balbino,Valdir de Queiroz (1) Leal,Tereza Cristina Arcanjo (1) Leal-Balbino,Tereza Cristina (1) Nascimento,Mirna Gisele Medeiros do (1) Oliveira,Maria Betânia Melo de (1) Sobreira,Marise (1) Mais... Palavra-chave Y. pestis (2) High pathogenicity island (1) Pgm (1) Plasmids (1) Probes (1) Psn (1) Virulence genes (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2006 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última The pgm locus and pigmentation phenotype in Yersinia pestis Genet. Mol. Biol. Leal-Balbino,Tereza Cristina; Leal,Nilma Cintra; Nascimento,Mirna Gisele Medeiros do; Oliveira,Maria Betânia Melo de; Balbino,Valdir de Queiroz; Almeida,Alzira Maria Paiva de. The pigmentation (pgm) locus is a large unstable area of the Yersinia pestis chromosome composed of a segment of iron acquisition (HPI) linked to a pigmentation segment. In this work we examined the mobility of HPI and the pigmentation segment in three Y. pestis isolates using successive subcultures on Congo red agar (CRA) plates. Strain P. CE 882 was shown to be highly stable while strains P. Exu 340 and P. Peru 375 dissociated into several phenotypes, PCR analysis showing evidence of changes in the pgm locus of the derived cultures. Strains P. Exu 340 and P. Peru 375 produced previously unreported cultures positive for the pesticin/yersiniabactin outer membrane receptor (psn+) but negative for the iron-regulated protein (irp2-), suggesting the occurrence... Tipo: Info:eu-repo/semantics/article Palavras-chave: Y. pestis; High pathogenicity island; Pgm; Psn. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100024 Homology among extra-cryptic DNA bands and the typical plasmids in Brazilian Yersinia pestis strains BJM Leal,Nilma Cintra; Sobreira,Marise; Leal,Tereza Cristina Arcanjo; Almeida,Alzira Maria Paiva de. Yersinia pestis, the etiologic agent of plague, harbors three well-characterized plasmids: pFra (90-110kb), pYV (70 kb) and pPst (9.5 kb). Furthermore, some extra-cryptic DNA bands have been observed in a number of wild strains from several foci of the world. Additional bands have also been reported in Brazilian strains. Looking for any relationship among these cryptic DNA bands and the three-prototypical plasmids, we analyzed twelve strains displaying different plasmid content. The DNA bands were hybridized by southern blot with probes directed at the genes caf1, lcrV and pla located respectively on the plasmids pFra, pYV and pPst. The probes were constructed by PCR amplification and labeled with digoxigenin. The Pla probe hybridized with its target... Tipo: Info:eu-repo/semantics/article Palavras-chave: Y. pestis; Plasmids; Probes; Virulence genes. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100006 Registros recuperados: 2 Primeira ... 1 ... Última

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