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Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies Genet. Mol. Biol.
Paiva,Marcelo Henrique Santos; Guedes,Duschinka Ribeiro Duarte; Leal,Walter Soares; Ayres,Constância Flávia Junqueira.
Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).
Tipo: Info:eu-repo/semantics/article Palavras-chave: ZIKV; RT-PCR; Aedes aegypti; Vector competence.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400597
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Overview of Zika virus (ZIKV) infection in regards to the Brazilian epidemic BJMBR
Slavov,S.N.; Otaguiri,K.K.; Kashima,S.; Covas,D.T..
Zika virus (ZIKV), a mosquito-borne flavivirus, belongs to the Flaviviridae family, genus Flavivirus. ZIKV was initially isolated in 1947 from a sentinel monkey in the Zika forest, Uganda. Little clinical importance was attributed to ZIKV, once only few symptomatic cases were reported in some African and Southeast Asiatic countries. This situation changed in 2007, when a large outbreak was registered on the Yap Island, Micronesia, caused by the Asian ZIKV lineage. Between 2013 and 2014, ZIKV spread explosively and caused many outbreaks in different islands of the Southern Pacific Ocean and in 2015 autochthonous transmission was reported in Brazil. Currently, Brazil is the country with the highest number of ZIKV-positive cases in Latin America. Moreover,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zika virus; ZIKV; Transmission; Epidemiology; Congenital infection; Brazil.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500201
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