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Optimization of liquid fermentation conditions for biotransformation zein by Cordyceps militaris 202 and characterization of physicochemical and functional properties of fermentative hydrolysates BJM
Yang,Shuang; Zheng,Mingzhu; Cao,Yong; Dong,Yanjiao; Yaqoob,Sanabil; Liu,Jingsheng.
Abstract Cordyceps militaris 202 is a potential fungus for biotransformation zein, due to its various proteases, high tolerance and viability in nature. In this article, single factor experiment and response surface methodology were applied to optimize the liquid fermentation conditions and improve the ability of biotransformation zein. The optimized fermentation conditions were as follows: inoculum concentration of 19%, volume of liquor of 130 mL/500 mL and pH of 4.7. Under this condition, the degree of hydrolysis (DH) was 27.31%. The zein hydrolysates from fungi fermentation maintained a high thermal stability. Compared to the original zein, the zein hydrolysates were found to have high solubility, which most likely results in improved foaming and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zein; Degree of hydrolysis; Cordyceps militaris 202; Functionality.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300621
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Effect of modified clays on the structure and functional properties of biofilms produced with zein Ciênc. Tecnol. Aliment.
Tavares,Luciana Lopes; Almeida,Crislene Barbosa de; Caruso,Ícaro Putinhon; Cornélio,Marinônio Lopes; Lopes Filho,José Francisco.
The purpose of this study was to evaluate changes in the structure and some functional properties of biofilms added with modified clays (Cloisite® 15A and Cloisite® 30B) prepared by the casting method. The analysis of the microstructure of the films, scanning electron microscopy (SEM), Optical microscopy (MO), and Infrared Spectroscopy (FTIR) indicated that the addition of clay in the films resulted in the formation of a heterogeneous microstructure, microcomposite or tactoid. Due to the formation of a microcomposite structure, functional properties of the films added with both clays such as opacity, solubility, and permeability to water vapor (PVA), were not better than those of the control film. Thus, it was concluded that although it is possible to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Zein; Clays; Microcomposite; Tactoids.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000200017
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Effects of different packaging techniques on the microbiological and physicochemical properties of coated pumpkin slices Ciênc. Tecnol. Aliment.
AKSU,Filiz; URAN,Harun; DÜLGER ALTINER,Dilek; SANDIKÇI ALTUNATMAZ,Sema.
Abstract In this study the effects of zein film coating along with benzoic acid on the quality of sliced pumpkin samples, which were packaged with different techniques were investigated. The samples were allocated into different groups and were treated with different processes. Following processing, the samples were stored at +4 °C for twenty days. Physicochemical and microbiological analyses were carried out on the samples once every five days during the storage period. According to color analysis, the L* value was observed to have significantly decreased in the processed and packaged samples in comparison with the control group. Besides, a* and b* values increased in all groups. It was determined that zein film alone did not exhibit the expected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Edible film; Zein; Packaging; Pumpkin.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000300549
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Preparation of glycosylated hydrolysate by liquid fermentation with Cordyceps militaris and characterization of its functional properties Ciênc. Tecnol. Aliment.
YANG,Shuang; ZHENG,Mingzhu; LI,Sheng; XIAO,Yu; ZHOU,Qi; LIU,Jingsheng.
Abstract This study aimed to investigate the potential of a novel preparation strategy for glycosylated hydrolysate by liquid fermentation in order to provide a high-quality glycosylated hydrolysate. Protein solubility, surface hydrophobicity and antioxidant activity were evaluated. The results of Fourier transform infrared spectra demonstrated the occurrence of glycosylation reaction. The results of Fluorescence emission spectroscope revealed the decrease of tertiary conformation stability. Circular dichroism spectra revealed significant decrease in α-helix and increase in β-sheet and random coil by glycosylation reaction. In comparison with zein hydrolysate, glycosylated hydrolysate had lower surface hydrophobicity but higher in solubility and in vitro...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zein; Glycosylated hydrolysate; Cordyceps militaris; Conformational properties; Functional properties.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020005005212
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Dissecting the Opaque-2 regulatory network using transcriptome and proteome approaches along with enzyme activity measurements Scientia Agricola
Lefèvre,Agnès; Consoli,Luciano; Gaziola,Salete A.; Pellegrino,Ana Paula; Azevedo,Ricardo A.; Damerval,Catherine.
The Opaque-2 (O2) gene encodes a transcriptional activator specifically expressed for grain development of maize. o2 mutants have an opaque and chalky kernel, with a decrease in zein storage protein content, and an increase in the proportions of lysine and tryptophan. In this review, we present recent results investigating genetic properties of the O2 network, using transcriptome and proteome approaches, associated with measurements of activities of enzymes of the aspartate pathway and lysine degradation. The structural polymorphism at the O2 locus was investigated by RFLP in a collection of 51 maize inbred lines. Most polymorphic sites were found outside the coding regions. We then searched for relationships between RFLP polymorphism and (i) mRNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zein; Aspartate kinase; Lysine oxoglutarate reductase/saccharopine dehydrogenase; Polymorphism.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000200031
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