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| Lefèvre,Agnès; Consoli,Luciano; Gaziola,Salete A.; Pellegrino,Ana Paula; Azevedo,Ricardo A.; Damerval,Catherine. |
| The Opaque-2 (O2) gene encodes a transcriptional activator specifically expressed for grain development of maize. o2 mutants have an opaque and chalky kernel, with a decrease in zein storage protein content, and an increase in the proportions of lysine and tryptophan. In this review, we present recent results investigating genetic properties of the O2 network, using transcriptome and proteome approaches, associated with measurements of activities of enzymes of the aspartate pathway and lysine degradation. The structural polymorphism at the O2 locus was investigated by RFLP in a collection of 51 maize inbred lines. Most polymorphic sites were found outside the coding regions. We then searched for relationships between RFLP polymorphism and (i) mRNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zein; Aspartate kinase; Lysine oxoglutarate reductase/saccharopine dehydrogenase; Polymorphism. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000200031 |
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| Yang,Shuang; Zheng,Mingzhu; Cao,Yong; Dong,Yanjiao; Yaqoob,Sanabil; Liu,Jingsheng. |
| Abstract Cordyceps militaris 202 is a potential fungus for biotransformation zein, due to its various proteases, high tolerance and viability in nature. In this article, single factor experiment and response surface methodology were applied to optimize the liquid fermentation conditions and improve the ability of biotransformation zein. The optimized fermentation conditions were as follows: inoculum concentration of 19%, volume of liquor of 130 mL/500 mL and pH of 4.7. Under this condition, the degree of hydrolysis (DH) was 27.31%. The zein hydrolysates from fungi fermentation maintained a high thermal stability. Compared to the original zein, the zein hydrolysates were found to have high solubility, which most likely results in improved foaming and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zein; Degree of hydrolysis; Cordyceps militaris 202; Functionality. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300621 |
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