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| Marinangeli,Pablo; Deluchi,Bernardo; Ríos,Raúl; Franzone,Pascual; Galmarini,Claudio; Rosselló,Fernando; Curvetto,Néstor. |
| The aim of this work was to explore the possibility of obtaining transgenic plants of onion varieties cultivated in Argentina, starting from calli induced from mature zygotic embryos, using two strains of Agrobacterium tumefaciens as transfection vectors. Mature embryos from three varieties of 'Valenciana' onion, Torrentina, Cobriza INTA and Grano de oro were in vitro cultivated for callus induction. After three to four months an average of 57.4% success for the three varieties was reached. Transformation was carried out with Agl1 or LBA 4404 A. tumefaciens strains, both carrying a binary vector containing the marker gene gus a and the selection gene nptII. Selection was done in callus induction medium containing 10 mgL-1 geneticin during three... |
| Tipo: Journal article |
Palavras-chave: Agrobacterium tumefaciens; Allium cepa L.; Callus; Zygotic embryos. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300030 |
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| SANTA-CATARINA,CLAUDETE; MACIEL,SCHEILA DA CONCEIÇÃO; PEDROTTI,ENIO LUIZ. |
| Embriões zigóticos em diferentes estádios de desenvolvimento foram utilizados para avaliar o potencial de germinação e competência embriogênica in vitro. Frutos coletados entre 15 e 360 dias após a antese foram avaliados em relação ao diâmetro (mm), comprimento (mm) e massa fresca (g). Para avaliar a percentagem de germinação in vitro, embriões zigóticos em diferentes estádios de desenvolvimento foram inoculados em meio de cultura composto de sais minerais de Murashigue & Skoog (MS), ou em meio MS suplementado com 4,4 miM de BAP, sacarose (3%), ágar (0,6%), carvão ativado (0,15%). Para a indução de embriogênese somática, eixos embrionários em diferentes estádios de desenvolvimento foram inoculados em meio de cultura MS, suplementado com 2,4-D (0,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zygotic embryos; Somatic embryogenesis; In vitro germination; Woody plant; Lauraceae. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000500004 |
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| Souza,Rafaeli Aparecida Vieira de; Braga,Francyane Tavares; Setotaw,Tesfahun Alemu; Vieira Neto,João; Azevedo,Patrícia Helena de; Azevedo,Virgínia Helena de; Cançado,Geraldo Magela de Almeida. |
| The experiment was carried out to determine the appropriate dose of coconut water as supplement for in vitro cultivation of zygotic embryos from 19 olive genotypes. The isolated embryos of the olive seeds were immersed on culture medium containing 0 (control), 25, 50, and 100mL L-1 of fresh and sterile coconut water and kept for 45 days under controlled environment. The percentage of germination, shoot length, number of roots, number of leaves and number of internodes were measured for all 19 olive genotypes. The ANOVA of the parameters evaluated showed significant genotypes x doses of coconut water interaction for shoot length, number of leaves and number of internodes and the dose of 100mL L-1 produced the best results overall as indicated by the means... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Olea europaea; Culture medium; Complex mixture; Tissue culture; Zygotic embryos. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000200016 |
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