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Transgenic rice as a novel production system for Melanocarpus and Pycnoporus laccases Inra
de Wilde, C.; Uzan, E.; Zhou, Z.; Kruus, K.; Andberg, M.; Buchert, J.; Record, E.; Asther, M.; Lomascolo, A..
Laccases have numerous biotechnological applications, among them food processing. The widespread use of laccases has increased the demand for an inexpensive and safe source of recombinant enzyme. We explored the use of a rice-based system for the production of two fungal laccases derived from the ascomycete Melanocarpus albomyces and the basidiomycete Pycnoporus cinnabarinus. High-expression levels of active recombinant laccases were achieved by targeting expression to the endosperm of rice seeds. The laccase cDNAs were fused to a plant-derived signal sequence for targeting to the secretory pathway, and placed under the control of a constitutive seed-specific promoter fused to an intron for enhanced expression. This construct enabled the recovery of on...
Tipo: Journal Article Palavras-chave: PLANTE TRANSGENIQUE; BIOTECHNOLOGIE INDUSTRIELLE; PROPRIETE BIOCHIMIQUE; ASCOMYCETE; BASIDIOMYCETE LACCASE; RECOMBINANT FUNGAL ENZYME; RICE; HETEROLOGOUS PROTEIN; TRANSGENIC PLANT; BIOCHEMICAL PROPERTIES.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008c973f6c6&uri=/notices/prodinra1/2008/10/
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Natural and recombinant fungal laccases for paper pulp bleaching Inra
Sigoillot, C.; RECORD, E.; Belle, V.; ROBERT, J.L.; LEVASSEUR, A.; Punt, P.J.; Van Den Hondel, C.A.M.J.J.; Fournel, A.; Sigoillot, J.C.; ASTHER, M..
Three laccases, a natural form and two recombinant forms obtained from two different expression hosts, were characterized and compared for paper pulp bleaching. Laccase from Pycnoporus cinnabarinus, a well known lignolytic fungus, was selected as a reference for this study. The corresponding recombinant laccases were produced in Aspergillus oryzae and A. niger hosts using the lacI gene from P. cinnabarinus to develop a production process without using the expensive laccase inducers required by the native source. In flasks, production of recombinant enzymes by Aspergilli strains gave yields close to 80 mg l–1. Each protein was purified to homogeneity and characterized, demonstrating that the three hosts produced proteins with similar physico-chemical...
Tipo: Journal Article Palavras-chave: PULPE DE PAPIER; BIOTECHNOLOGIE INDUSTRIELLE; PROPRIETE PHYSICOCHIMIQUE LACCASE; RECOMBINANT PROTEIN.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007a0177074&uri=/notices/prodinra1/2010/09/
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