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Sequence analysis of three citrus viroids infecting a single Tunisian citrus tree (Citrus, reticulata, Clementine) Genet. Mol. Biol.
Elleuch,Amine; Khouaja,Fattouma Djilani; Hamdi,Imen; Bsais,Nabiha; Perreault,Jean-Pierre; Marrakchi,Mohamed; Fakhfakh,Hatem.
We report the nucleotide sequences of three citrus viroids belonging to three different genera: Citrus exocortis viroid (CEVd), Hop stunt viroid (HSVd) and Citrus viroid-III (CVd-III) isolated from a single natural infected Citrus reticulata var. Clementine tree growing in a tree nursery in Manouba (near Tunis Capital). We describe the sequence variability of these viroids from their natural host without using an alternative passage by an indicator host or an artificial inoculation. This work confirms that naturally occurring viroid infections contain a mixture of sequence variants. These are the first sequences of citrus viroids from Africa.
Tipo: Info:eu-repo/semantics/article Palavras-chave: CEVd; CVd-III; Citrus; HSVd; Quasi-species; Sequence variability; Viroid.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400020
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Citrus exocortis viroid and Hop Stunt viroid Doubly infecting grapevines in Brazil Trop. Plant Pathol.
Eiras,Marcelo; Targon,Maria Luisa P.N.; Fajardo,Thor V.M.; Flores,Ricardo; Kitajima,Elliot W..
Viroids, non-protein-coding small (246-401 nt) circular single-stranded RNAs with autonomous replication, are currently classified into two families. Within the family Pospiviroidae, Citrus exocortis viroid (CEVd) belongs to the genus Pospiviroid while Hop stunt viroid (HSVd) is the single member of the genus Hostuviroid. These pathogens are distributed worldwide and infect a large number of hosts. In Brazil, isolates of CEVd and HSVd have been detected in both citrus and grapevine. To characterize and study the genetic variability of these viroids, total RNA from leaves of grapevine Vitis vinifera 'Cabernet Sauvignon' and V. labrusca 'Niagara Rosada' from Bento Gonçalves, RS, was used as a template for RT-PCR amplification with specific primers for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CEVd; HSVd; Pospiviroid; Hostuviroid; Pospiviroidae.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582006000500002
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Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR Ciencia e Investigación Agraria
Camps,Rocío; Castro,Mónica; Besoain,Ximena.
R. Camps, M. Castro, and X. Besoain. 2014. Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR. Cien. Inv. Agr. 41(2): 255-261. Citrus tristeza virus (CTV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd) are pathogens that affect citrus trees in Chile. The simultaneous use of a rapid diagnostic method with the effective cleaning of infected budwood is a powerful preventative method for controlling viral diseases. The objective of this study was the multiple detection of CTV, CEVd, and HSVd, through bio-amplification in Arizona 861-S1 citron plants, combined with two incubation temperatures and multiple RT-PCR in two steps. Citron indicator plants were inoculated with the three pathogens, both individually and...
Tipo: Journal article Palavras-chave: CEVd; Citrus; CTV; Diagnostic methods; HSVd; Rapid detection.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000200012
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