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Methodology for fast evaluation of Bacillus thuringiensis crystal protein content 58
Alves,Lúcia M. Carareto; Lemos,Manoel V. F..
The development of the production and use of Bacillus thuringiensis in Brazil at a commercial scale faces certain difficulties, among them the establishment of efficient methodologies for the quantitation of toxic products to be commercialized. Presently, the amount of toxin is given in percentage by analyzing the samples total protein content. Such methodology however, does not measure the actual amount of active protein present in the product, since most strains express different endotoxin genes and might even produce b-toxin. Since the various types of toxins exhibit different antigenic characteristics, this work has as objective the utilization of fast immunological techniques to quantify the level of crystal protein. Crystal protein produced by a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis; Crystal protein; Immunological methods.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300012
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Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy 74
Lima,A.S.G.; Guidelli,A.M.; Abreu,I.L.; Lemos,M.V.F..
PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: REP; ERIC; Crystal protein; Parasporal bodies.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200017
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Characterization and selection of Bacillus thuringiensis isolates effective against Sitophilus oryzae 63
Silva,Najara da; Thuler,Ana Maria Guidelli; Abreu,Irlan Leite de; Davolos,Camila Chiaradia; Polanczyk,Ricardo Antonio; Lemos,Manoel Victor Franco.
The entomopathogenic bacterium Bacillus thuringiensis is a control agent with toxic and environmental characteristics that allows the control of pest insects according to the Integrate Pest Management (IPM) precepts. In order to find new strains, potentially toxic to Sitophilus oryzae L. 1763 (Coleoptera: Curculinidae), 1.073 strains of B. thuringiensis from parts of Brazil were used. Genetic material was extracted with InstaGene Matrix kit, used for the amplification of sequences in Polymerase chain reaction (PCR), and viewed in 1.5% agarose gel. The gene cry35Ba class was represented by 60 B. thuringiensis isolates (5.6%), which were then subjected to bioassays with S. oryzae larvae. Among the isolates studied, four caused more than 50% mortality in...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Coleoptera; Curculionidade; Crystal protein; Entomopathogenic bacterium.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400015
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