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Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress Electron. J. Biotechnol.
Miao,Hongxia; Wang,Yuan; Liu,Juhua; Jia,Caihong; Hu,Wei; Sun,Peiguang; Jin,Zhiqiang; Xu,Biyu.
Background Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to analysis the sequence characterization of banana ASR1, and identify its expression patterns and function under salt stress using quantitative real-time PCR (qPCR) and overexpression in Arabidopsis. The purpose was to evaluate the role of banana ASR1 to salt stress tolerance employed by plants. Results A full-length cDNA isolated from banana fruit was named MaASR1, and it had a 432 bp open reading frame (ORF) encoding 143 amino acids. MaASR1 was preferential...
Tipo: Journal article Palavras-chave: ASR; Banana; Expression patterns; Salt resistance.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600006
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Identification and expression analysis of two Wnt4 genes in the spotted scat (Scatophagus argus) Electron. J. Biotechnol.
Chen,Jianhua; Yinglei,Li; Zhang,Junbin; Liu,Huifen; Li,Yongqi.
Background: WNT4 is a protein that plays a crucial role in ovarian differentiation and development in mammals, with a relatively well understood function in mammalian gonadal differentiation. The role of WNT4 in teleost fish; however, remains unclear. In the present study, cDNAs of Wnt4a and Wnt4b were cloned and characterized in the spotted scat. The expression patterns of two Wnt4 genes in the gonads at different stages of development and in fish after treatment with 17a-methyltestosterone (MT) were investigated. Results: The tissue distribution showed that Wnt4a was expressed in various tissues, including the gonads, gills, spleen, brain, and fin. Interestingly, Wnt4b not only was expressed in the gills, brain, and spleen, but also was obviously...
Tipo: Journal article Palavras-chave: Androgen; Expression patterns; Scatophagus argus; Tissue expression; Wnt4.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200004
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Expression analysis of vasa in Asian paddle crab (Charybdis japónica) exposed to Bisphenol A Electron. J. Biotechnol.
Chen,Jianhua; Wang,Cuihua; Gao,Huan; Yan,Binlun.
Background: Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) with a weak estrogen-like activity in fish that is found ubiquitously in aquatic environments. However, there has been little study about BPA on the endocrine disrupting effects of crab. In the present study, cDNA of vasa was cloned and characterized in the Charybdis japonica. Histological structures of testis and expression patterns of vasa gene in the testis of C. japonica after treatment with BPA were investigated. Results: The cDNA of vasa is composed of 3051 bp with a 2166 bp open reading frame encoding 721 AA. The deduced amino acid sequence contained eight conserved domains of the DEAD-box protein family. The tissue distribution showed that vasa mRNA was specifically expressed...
Tipo: Journal article Palavras-chave: Aquatic environments; BPA; DEAD-box; Endocrine disrupting; Expression analysis; Expression patterns; Histological observation; Vasa; Vasa cDNA; Vasa gene; Vasa mRNA.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600008
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