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Registros recuperados: 131 | |
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Moraes,Luiza; Rosa,Gabriel Martins da; Souza,Michele da Rosa Andrade Zimmermann de; Costa,Jorge Alberto Vieira. |
ABSTRACT Microalgae are efficient at using solar energy to turn CO2 and nutrients into biomass containing lipids, proteins, carbohydrates and other compounds that may be used to produce bioproducts for human and animal consumption and pharmaceutical use. The aim of this study was to assess the effect of the NaNO3 and NaCl concentration on the growth kinetics, the biomass composition and the ability to biofix CO2 using the microalga Spirulina sp. LEB 18. The assays were carried out according to a 22 central composite design (CCD) with different concentrations of NaNO3 (1.25, 1.88 and 2.50 g L-1) and NaCl (1.00, 15.0 and 30.0 g L-1). The assays were carried out in 2 L vertical tubular photobioreactors at 30°C, 12 h light/dark and an injection of 12.0% v/v of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CO2; Fixation; Growth kinetics; Microalgae; Nutrients. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100410 |
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De Oliveira-júnior, Raimundo Gonçalves; Nicolau, Elodie; Bonnet, Antoine; Prunier, Grégoire; Beaugeard, Laureen; Joguet, Nicolas; Thiéry, Valérie; Picot, Laurent. |
Melanoma is an aggressive tumor with invasive and metastatic potential, frequently exhibiting multidrug resistance mechanisms. In our continuous search for antimelanoma molecules, we have identified some effective marine compounds capable of not only inducing cell death, but also of sensitizing chemoresistant tumor cells to clinically used anticancer drugs. In this report, the cryptophyte Rhodomonas salina (Wislouch) D.R.A.Hill & R.Wetherbee, Pyrenomonadaceae, was chemically investigated in order to identify pigments efficiently inhibiting melanoma cells proliferation. All pharmacological tests were performed on A2058 cells expressing the oncogenic BRAF V600E mutation and resistant to dacarbazine treatment. Flash chromatography of R. salina ethanol... |
Tipo: Text |
Palavras-chave: Alloxanthin; Chemosensitivity; Crocoxanthin; Cutaneous melanoma; Drug resistance; Microalgae. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00630/74194/73918.pdf |
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Neves,Cristina; Maroneze,Mariana Manzoni; Santos,Aline Meireles dos; Francisco,Erika Cristina; Wagner,Roger; Zepka,Leila Queiroz; Jacob-Lopes,Eduardo. |
ABSTRACT This study aimed to evaluate third generation biodiesel production by microalgae Phormidium autumnale using cassava processing wastewater as a platform. Experiments were performed in a heterotrophic bubble column bioreactor. The study focused on the evaluation of the bioreactor (batch and fed-batch) of different operational modes and the analysis of biofuel quality. Results indicate that fed-batch cultivations improved system performance, elevating biomass and oil productions to 12.0 g L−1 and 1.19 g L−1, respectively. The composition of this oil is predominantly saturated (60 %) and monounsaturated (39 %), resulting in a biodiesel that complys with U.S., European and Brazilian standards. The technological route developed indicates potential for... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microalgae; Cyanobacteria; Agroindustrial wastewater; Biofuel. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500412 |
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Cellamare, M.; Duval, C.; Drelin, Y.; Djediat, C.; Touibi, N.; Agogue, H.; Leboulanger, C.; Ader, M.; Bernard, C. |
The saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean) is dominated by the bloom-forming cyanobacterium Arthrospira. However, the rest of the phototrophic community remains underexplored because of their minute dimension or lower biomass. To characterize the phototrophic microorganisms living in this ecosystem considered as a modern analog of Precambrian environments, several strains were isolated from the water column and stromatolites, and analyzed using the polyphasic approach. Based on morphological, ultrastructural and molecular (16S rRNA gene, 18S rRNA gene, 16S-23S ITS region, cpcBA-IGS locus) methods, seven filamentous cyanobacteria and the prasinophyte Picocystis salinarum were identified. Two new genera and four new cyanobacteria... |
Tipo: Text |
Palavras-chave: Cyanobacteria; Microalgae; Crater-lake; Saline-alkaline lake; Polyphasic approach; Arthrospira. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00443/55469/56991.pdf |
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Olofsson, Martin; Lamela, Teresa; Nilsson, Emmelie; Berge, Jean-pascal; Del Pino, Victoria; Uronen, Pauliina; Legrand, Catherine. |
Instead of sole nutrient starvation to boost algal lipid production, we addressed nutrient limitation at two different seasons (autumn and spring) during outdoor cultivation in flat panel photobioreactors. Lipid accumulation, biomass and lipid productivity and changes in fatty acid composition of Nannochloropsis oculata were investigated under nitrogen (N) limitation (nitrate:phosphate N:P 5, N:P 2.5 molar ratio). N. oculata was able to maintain a high biomass productivity under N-limitation compared to N-sufficiency (N:P 20) at both seasons, which in spring resulted in nearly double lipid productivity under N-limited conditions (0.21 g L−1 day−1) compared to N-sufficiency (0.11 g L−1 day−1). Saturated and monounsaturated fatty acids increased from 76% to... |
Tipo: Text |
Palavras-chave: Microalgae; Outdoor; Nannochloropsis; Large-scale; Lipids; Fatty acids; Nitrogen manipulation; Seasonal changes; Biofuels; High value products. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00184/29564/27902.pdf |
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Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret, Jean-paul. |
Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins... |
Tipo: Text |
Palavras-chave: Microalgae; Biotechnology; Lipid; Nitrogen; Proteomic; Selection. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00179/29012/27470.pdf |
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Cordier, Clémence; Guyomard, Killian; Stavrakakis, Christophe; Sauvade, Patrick; Coelho, Franz; Moulin, Philippe. |
The culture of microalgae is important for the production and maintenance of bivalves. One of the major challenges is to maintain the reliability of microalgae forages over the long term. The aim of this work is to use Ultrafiltered (UF) seawater to cultivate them. Thus, cultures in a volume of 300 L of 2 species of microalgae Tetraselmis and T-isochrysis, were monitored in UF water (membrane pore size: 20 nm) and in sea water usually used on the Ifremer mollusk experimental platform of Bouin (France) (Prefiltration, 3 filtrations and 2 UV). The major result is the securing of microlagae cultures with the absence of parasites in all cultures supplied with ultrafiltered water, unlike analyses of the various control cultures. In the case of T-isochrysis, 3... |
Tipo: Text |
Palavras-chave: Microalgae; Ultrafiltration; Water Purification; Shellfish Production. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00630/74182/73785.pdf |
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Dupraz, Valentin; Stachowski-haberkorn, Sabine; Wicquart, Jérémy; Tapie, Nathalie; Budzinski, Hélène; Akcha, Farida. |
Pesticides used in viticulture create a potential risk for the aquatic environment due to drift during application, runoff and soil leaching. The toxicity of sixteen pesticides and one metabolite were evaluated on the growth of two marine microalgae, Tisochrysis lutea and Skeletonema marinoi, in 96-h exposure assays conducted in microplates. For each substance, concentrations of stock solutions were analytically measured and abiotic assays were performed to evaluate the chemical stability of pesticides in microplates. For two chemicals, microalgae exposures were run simultaneously in microplates and culture flasks to compare EC50 calculated from the two exposure systems. Results from chemical analyses demonstrated the low stability of hydrophobic... |
Tipo: Text |
Palavras-chave: Microalgae; Microplates; Screening; Pesticides; Toxicity; Exposure characterisation. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00474/58558/61113.pdf |
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Nassif, L. Abi; Rioual, S.; Trepos, Rozenn; Fauchon, M.; Farah, W.; Hellio, C.; Abboud, M.; Lescop, B.. |
Microorganisms have the ability to settle on nearly all man-made surfaces in contact with seawater and subsequently to form biofilm. Biofilms control and removal is necessary in the sectors of maritime transport, energy... In this work, we present the development of new pure calcium, zinc or copper alginate, but also mixed Ca/Cu and Ca/Zn alginate hydrogels. These materials have been evaluated for their potential inhibition of adhesion of two key biofilm-forming microalgae Halamphora coffeaeformis and Cylindrotheca closterium). All the tested materials have presented high adhesion inhibition about 80%). Copper-base materials present a high toxicity against H. coffeaeformis. Pure zinc alginate is also toxic for this strain. However, the addition of calcium... |
Tipo: Text |
Palavras-chave: Biofilm; Adhesion; Alginate; Zinc; Biomaterials; Microalgae. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00605/71671/70168.pdf |
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Bartozek,Elaine Cristina Rodrigues; Bueno,Norma Catarina; Ludwig,Thelma Alvim Veiga; Tremarin,Priscila Izabel; Nardelli,Margaret Seghetto; Rocha,Angélica Cristina Righetti da. |
This study represents a taxonomic survey of class Bacillariophyceae diatoms found in samples collected in the Iguaçu River (Iguaçu National Park, municipality of Foz do Iguaçu, in the state of Paraná, Brazil) between August 2007 and July 2008. Two sampling stations were utilized, the first located upstream of Iguaçu Falls (Cais Bananeiras Station) and second downstream of the falls (Cais Macuco Safari Station). The study resulted in the identification of 86 taxa, including 74 at the specific level and seven taxonomic varieties. Two of these had never before been documented in the state of Paraná: Gomphonema gibberum Hustedt and Pinnularia meridiana Metzeltin & Krammer. Nine are first-time records for Brazil: Cymbella charrua Metzeltin,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Taxonomy; Microalgae; Lotic system; State of Paraná. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000100012 |
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Teixeira,Priscila F.; Kaminski,Sonia M.; Avila,Tatiana R.; Cardozo,Alessandro P.; Bersano,José G.F.; Bianchini,Adalto. |
Egg production in the copepod Acartia tonsa was evaluated using different densities of the microalgae Thalassiosira weissflogii, Chaetoceros muelleri and Isochrysis galbana. Male and female were kept under controlled conditions (salinity 30, 20°C, photoperiod 12L:12D), acclimated to the experimental conditions and left over a period of 24 h to allow copulation. Algal densities tested were equivalent in biovolume and corresponded to 0, 2.5, 5, 10, 20, 40 and 60.10³ cells.mL-1 of T weissflogii. Ten acclimated female were separated, transferred to glass bottles and exposed for further 24 h to the corresponding experimental medium. After this period, the eggs were fixed and counted. Copepod egg production reached a threshold value when T weissflogii, C.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Egg production; Acartia tonsa; Microalgae. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200009 |
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Rossa-Feres,Denise de C.; Jim,Jorge; Fonseca,Mariluce Gonçalves. |
The diet of tadpoles of 13 anuran species was determined to verify whether food resource partitioning occurs and whether the degree of diet similarity is related to taxonomic affinity. Tadpoles of all species studied were mainly herbivorous, except for these of Leptodactylus fuscus (Schneider, 1799) which were mycophagous. Although some species had exclusive items in their diet, most tadpole species ingested the same items, but differed in the amount of each item consumed. Two guilds were found: tadpoles that feed on diatoms on the pond bottom, and tadpoles that feed on Oedogonium Link, 1820 algae in midwater. Diet similarity was related to the taxonomic relationship, microhabitat and feeding behavior of tadpoles indicating that the community organization... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Community ecology; Food partitioning; Microalgae. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752004000400003 |
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Sousa, Celia; Compere, Chantal; Dreanno, Catherine; Crassous, Marie-pierre; Gas, Fabienne; Baus, Beatrice; Perrot, Hubert. |
In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes. |
Tipo: Text |
Palavras-chave: Alexandrium minutum; Quartz microbalance; Microalgae; Immunosensor. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00194/30492/28897.pdf |
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Cognie, B; Barille, Laurent. |
Bivalves, by releasing inorganic nutrients, can stimulate the growth of the primary producers on which they feed. Dissolved excretion and mineralization of biodeposits (pseudofaeces and faeces), two indirect processes related to their feeding activity, have already been identified. This study investigated whether microalgal growth is also dependent on the mucus secreted by bivalves and rejected with their biodeposits. An experimental study based on algal growth tests was conducted to determine whether the mucus coating the pseudofaeces and faeces of the oyster Crassostrea gigas (Thunberg) could stimulate the growth of nine marine microalgae: a Prasinophyceae [Tetraselmis suecica (Butcher)] and eight Diatomophyceae [Amphora sp. (Ehrenberg), Chaetoceros sp.... |
Tipo: Text |
Palavras-chave: Bivalve; Microphyte; Mucus; Fèces; Pseudofèces; Bivalve; Microalgae; Mucus; Faeces; Pseudofaeces. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00325/43607/43926.pdf |
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Carrier, Gregory; Baroukh, Caroline; Rouxel, Catherine; Duboscq-bidot, Laetitia; Schreiber, Nathalie; Bougaran, Gael. |
Tisochrysis lutea is a microalga species currently used in aquaculture as a feed for shellfish, oysters and shrimps. It also has many other potential industrial applications, such as the production of neutral lipids for biofuels or the production of ω-3 fatty acids for nutraceuticals (human food complements). To efficiently exploit the potential of this microalga, however, higher lipid productivities are needed. To this end, improvement programs need to be developed and optimized. The diversity of strains available in microalgae has not yet been exploited in such improvement programs. In this study, the intra-strain diversity was observed and exploited to increase neutral lipid productivity. New clonal strains with higher neutral lipid productivity were... |
Tipo: Text |
Palavras-chave: Microalgae; Genome; Improvement program; Lipids; Tisochrysis lutea. |
Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00411/52204/52927.pdf |
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Registros recuperados: 131 | |
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