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Registros recuperados: 216
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Efficiency of RAPD and ISSR markers in assessing genetic variation in Arthrocnemum macrostachyum (Chenopodiaceae) BABT
Saleh,Basel.
The goal of this study was to investigate the molecular characterization of A. macrostachyum genotypes grown in the Western coast of Syria using RAPD and ISSR techniques. PCR amplifications with 20 RAPD primers gave an average of 9.25 selected markers/ primer, with a maximum of 17 (OPG05) and a minimum of four (OPA02). Percentage of polymorphic bands ranged from 40 to 100% according to RAPD primers tested. Among the 185 selected bands, 160 (84.96%) were polymorphic. The amplification with seven ISSR primers generated 88 bands, and 80 (90.91%) were polymorphic. (CA)8RG and (AG)8GTG ISSR primers tested in this study yielded highly informative patterns. RAPD and ISSR fragment sizes ranged from 0.2-3 kb. Based on this study, the use of RAPD and ISSR...
Tipo: Info:eu-repo/semantics/article Palavras-chave: A. macrostachyum; Genetic diversity; Genotype; ISSR; Polymorphism; RAPD.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500002
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Enzyme polymorphism between Oryza sativa and O. minuta and its use to monitor introgression in O. sativa/O. minuta backcross derivatives International Rice Research Institute
Romero, Gabriel Ortega.
87 l. : ill. Thesis (M.S.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Plant genetics; Enzymes; Polymorphism; Oryza minuta; Backcrossing; Introgression.
Ano: 1989 URL: http://hdl.handle.net/123456789/1121
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Enzyme polymorphism in rice : relation to pollen fertility and blast resistance International Rice Research Institute
Dekuku, Roger Chris.
162 l. : ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Enzymes; Polymorphism; Plant fertility; Blast disease; Disease resistance; Plant genetics.
Ano: 1991 URL: http://hdl.handle.net/123456789/953
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EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.) Electron. J. Biotechnol.
Song,Guo Qi; Li,Meng Jun; Xiao,Han; Wang,Xing Jun; Tang,Rong Hua; Xia,Han; Zhao,Chuan Zhi; Bi,Yu Ping.
Making use of the gene resources of wild type peanuts is a way to increase the genetic diversity of the cultivars. Marker assisted selection (MAS) could shorten the process of inter-specific hybridization and provide a possible way to remove the undesirable traits. However, the limited number of molecular markers available in peanut retarded its MAS process. We started a peanut ESTs (Expressed Sequence Tags) project aiming at cloning genes with agronomic importance and developing molecular markers. In this study we found 610 ESTs that contained one or more SSRs from 12,000 peanut ESTs. The most abundant SSRs in peanut are trinucleotides (66.3%) SSRs and followed by dinucleotide (28.8%) SSRs. AG/TC (10.7%) repeat was the most abundant and followed by CT/GA...
Tipo: Journal article Palavras-chave: Arachis hypogaea L.; Cultivars; EST-SSR; Polymorphism; Wild-type peanut.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300007
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Estudo de algumas características fenotípicas relacionadas ao desempenho de tourinhos da raça Canchim em confinamento: 1. Desenvolvimento ponderal e polimorfismo para cor da mufla. Repositório Alice
COUTINHO FILHO, J. L. V.; PERES, R. M.; JUSTO, C. L.; ALENCAR, M. M. de; RAZOOK, A. G.; SILVA, L. R. M. da; SIQUEIRA, P. A..
O objetivo do trabalho foi avaliar uma possivel associação entre polimorfismo para cor da mufla e desempenho de tourinhos Canchim. O trabalho foi realizado em dois locais diferentes. Na Fazenda São Jorge (Cedral-SP), foram utilizados 51 aimais inteiros da raça Canchim com médias de 20 meses de idade e 327 kg de peso vivo ao inicio do período, e classificados em 3 grupos em relação a cor de mufla (escura, rosada e pigmentação intermediaria). O mesmo procedimento for feito para 105 animais que estavam participando da prova de Ganho de Peso na Estação Experimental de Zootecnia de Sertãozinho (SP), iniciando a prova com 210 dias e terminando com 378 dias de idade. Os resultados mostraram que não existiu associação significativa entre os grupos com diferentes...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Gado de Corte; Cor da mufla; Ganho de peso; Polimorfismo; Beef cattle; Gain of weight; Polymorphism.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/43280
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Etude de la variabilité génétique de populations naturelles d'huîtres creuses Crassostrea gigas (Thunberg) du littoral Atlantique Français ArchiMer
Gras, Marie-paule; Gras, Paul.
The aductor muscle proteins of Japaneses oysters C. gigas collected from three different geographical wild stocks were studied by acrylamid gel electrophoreseis. The first results reveal a biochemical polymorphism of a protein fraction
Tipo: Text Palavras-chave: Crassostrea gigas; Oysters; Genetic; Polymorphism; Genetic population; Crassostrea gigas; Huîtres; Génétique; Polymorphisme; Populations naturelles.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/acte-2952.pdf
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Evaluation of polymorphism in ten microsatellite loci in Uruguayan sheep flocks Genet. Mol. Biol.
Tomasco,Ivanna; Wlasiuk,G.; Lessa,E.P..
The allele frequencies of 10 microsatellite loci previously described for sheep as BM1314, BM6526, OarFCB128, OarHH64, OarCP20, OarHH47, OarFCB48, OarHH35, OarHH72 and BM2508 were estimated for the Uruguayan flocks. A representative sample of 101 individuals composed by the two predominant breeds (76% Corriedale and 24% Australian Merino) was used. The sample did not show a significant tendency towards substructuring, in spite of presenting some significantly different allele frequencies between races. The Corriedale sample presents three loci in which the presence of null alleles is possible. The markers were highly variable, showing between 7 and 15 alleles each. The Polymorphism Information Content Index ranged from 0.63 to 0.87 and the Exclusion...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sheep; Polymorphism; Microsatellites.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100008
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Evaluation of the association between the TAS1R2 and TAS1R3 variants and food intake and nutritional status in children Genet. Mol. Biol.
Melo,Silvia V.; Agnes,Grasiela; Vitolo,Márcia R.; Mattevi,Vanessa S.; Campagnolo,Paula D.B.; Almeida,Silvana.
Abstract Taste perception plays a key role in determining individual food preferences and dietary habits and may influence nutritional status. This study aimed to investigate the association of TAS1R2 (Ile191Val - rs35874116) and TAS1R3 (-1266 C/T - rs35744813) variants with food intake and nutritional status in children followed from birth until 7.7 years old. The nutritional status and food intake data of 312 children were collected at three developmental stages (1, 3.9 and 7.7 years old). DNA was extracted from blood samples and the polymorphisms were analyzed by real-time polymerase chain reactions (qPCR) using hydrolysis probes as the detection method. Food intake and nutritional status were compared among individuals with different single nucleotide...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food intake; Polymorphism; TAS1R2 gene; TAS1R3 gene; Taste receptors.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300415
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Expression and polymorphism of watermelon fruit ESTs National Institute of Agronomic Research
Levi, A.; Wechter, W.P.; Harris, K.R.; Davis, A.R.; Fei, Z.; Giovannoni, J.J.; Trebitsh, T.; Salman, A.; Hernandez, A.; Thimmapuram, J.; Tadmor, Y.; Portnoy, V.; Katzir, N..
Over 8,000 ESTs were generated for watermelon and were assembled into 4,700 EST-unigenes (http://www.icugi.org). Microarray and Real-Time PCR analyses were used to examine differential expression of 832 of these EST-unigenes in developing and ripening watermelon fruit. RNA was isolated from watermelon fruits(in three sequential stages of development and ripening) and from young leaves of field grown plants (during three consecutive years). The microarray analysis identified 335 EST-unigenes differentially expressed (by at least two-fold when compared to leaf) in watermelon fruit during the early, ripening, or mature stage. A considerable number of these watermelon ESTs are associated with vascular system differentiation and development, ethylene...
Tipo: Conference Paper Palavras-chave: Watermelon; Microarray; Polymorphism; PCR analysis; Expressed sequence tag; EST; Gene expression.
Ano: 2008 URL: http://hdl.handle.net/2174/216
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Extending the repertoire of microsatellite markers for genetic linkage mapping and germplasm screening in chickpea Open Agri
Varshney, R.K.,( Rajeev K. Varshney ).
Palavras-chave: Genetic structures; Chickpeas; Genomes; Cloning; Chromosome mapping; Plasmids; Polymorphism; DNA; Genotypes; Genetic markers.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3338
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Extreme homogeneity among Brazilian wheat genotypes determined by RAPD markers PAB
FREITAS,LORETA BRANDÃO DE; JERUSALINSKY,LEANDRO; BONATTO,SANDRO LUIS; SALZANO,FRANCISCO MAURO.
Random amplified polymorphic DNA markers (RAPD) were used to estimate the variability of 14 genotypes of Brazilian wheat (Triticum aestivum L.), using a set of 50 random 10mer primers. A total of 256 reproducibly scorable DNA amplification products were obtained from 48 of the primers, 83% of which were polymorphic. Genetic distances among genotypes were calculated and a dendrogram and a principal coordinates analysis showing the genetic relationships among them were obtained. Despite the low variability found (average genetic distance of 27%), two groups of genotypes could be identified, which probably reflect how they were formed. Studies such as this one may be important in the planning and development of future improvement programs for this plant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Genetic variation; Polymorphism; Breeding methods.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000001100018
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Factor 9 de crecimiento y diferenciación asociado al índice de prolificidad en la oveja pelibuey. Colegio de Postgraduados
Pérez Ruíz, Elizabeth.
El Factor 9 de Crecimiento y Diferenciación (GDF9) es miembro de la súperfamilia β de factores de crecimiento (TGFβ) y su expresión en el ovocito es esencial para el desarrollo y crecimiento folicular. Diferentes mutaciones en el gen GDF9 han sido asociadas con el incremento de tasa de ovulación y/o prolificidad en algunas razas de ovejas, por lo que el objetivo de este estudio fue la búsqueda de polimorfismos de una sola base (SNPs) en el gen GDF9, así como la asociación entre polimorfismos del gen GDF9 y el índice de prolificidad en ovejas de la raza Pelibuey. Se tomaron muestras sanguíneas de la vena yugular de 16 ovejas y fueron conservadas en papel FTA® (Whatman Mini Card). El exón dos del gen fue amplificado mediante la técnica de Polimerase Chain...
Palavras-chave: Análisis de secuencias; GDF9; Mutación; Polimorfismo; PCR; SNP; Sequence analysis; Mutation; Polymorphism; Maestría; Ganadería.
Ano: 2012 URL: http://hdl.handle.net/10521/1712
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Foundational errors in the Neutral and Nearly-Neutral theories of evolution in relation to the Synthetic Theory: Is a new evolutionary paradigm necessary? Biol. Res.
Valenzuela,Carlos Y.
The Neutral Theory of Evolution (NTE) proposes mutation and random genetic drift as the most important evolutionary factors. The most conspicuous feature of evolution is the genomic stability during paleontological eras and lack of variation among taxa; 98% or more of nucleotide sites are monomorphic within a species. NTE explains this homology by random fixation of neutral bases and negative selection (purifying selection) that does not contribute either to evolution or polymorphisms. Purifying selection is insufficient to account for this evolutionary feature and the Nearly-Neutral Theory of Evolution (N-NTE) included negative selection with coefficients as low as mutation rate. These NTE and N-NTE propositions are thermodynamically (tendency to random...
Tipo: Journal article Palavras-chave: Fixation; Loss; Neutral evolution; Polymorphism; Randomness; Selectiveness.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200001
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French scallops: A new host for ostreid herpesvirus-1 ArchiMer
Arzul, Isabelle; Nicolas, Jean-louis; Davison, Andrew J.; Renault, Tristan.
Sporadic high mortalities were reported among larval French scallops (Pecten maximus). Electron microscopy of moribund larvae revealed particles with the characteristics of a herpesvirus in association with cellular lesions. PCR and DNA sequencing showed that the virus is a variant of ostreid herpesvirus-1 that has already been described in clams and oysters. This is the first description of a herpesvirus infection of a scallop species. The virus was transmitted successfully from an extract of infected scallop larvae to uninfected scallop or oyster (Crassostrea, gigas) larvae, demonstrating that it is able to infect both species. Detection of viral DNA in asymptomatic adult scallops by in situ hybridisation indicates that the herpesvirus may have been...
Tipo: Text Palavras-chave: Bivalve; Host range; Transmission; Polymorphism; OsHV 1; Pecten maximus; Scallop; Herpesvirus.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-739.pdf
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Frequency of adult type-associated lactase persistence LCT-13910C/T genotypes in the Czech/Slav and Czech Roma/Gypsy populations Genet. Mol. Biol.
Hubácek,Jaroslav A.; Adámková,Věra; Šedová,Lenka; Olišarová,Věra; Adámek,Václav; Tóthová,Valérie.
Abstract Lactase non-persistence (leading to primary lactose intolerance) is a genetically dependent inability to digest lactose in adulthood. As part of the human adaptation to dairying, the human lactase LCT-13910C/T mutation (which propagates adult expression of lactase) developed, spread and participated in the adaptation to dairying. This variant is associated with lactase activity persistence, and its carriers are able to digest lactose. We compared the frequencies of lactase 13910C/T (rs4988235) genotypes in Czechs/Slavs (N = 288) and Czech Gypsies/Roma (N = 300), two ethnically different groups where this polymorphism has not yet been analysed. Allelic frequencies significantly differed between the populations (p < 0.0001). In Czechs/Slavs, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactose; Lactase persistence; Polymorphism; Czech/Slav; Czech Gypsy/Roma.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300450
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Gene polymorphism of interleukin 1 and 8 in chronic gastritis patients infected withHelicobacter pylori J. Venom. Anim. Toxins incl. Trop. Dis.
Caleman Neto,Agostinho; Rasmussen,Lucas T; Labio,Roger W de; Queiroz,Valdeir F de; Smith,Marília de AC; Viani,Gustavo A; Payão,Spencer LM.
Background : Epidemiological investigations have indicated thatHelicobacter pyloriinduces inflammation in the gastric mucosa regulated by several interleukins. The genesIL1BandIL8are suggested as key factors in determining the risk of gastritis. The aim of this paper was to evaluate the association of gene polymorphism of interleukin-1 and interleukin-8 with chronic gastrits inH. pyloriinfected patients. A total of 60 patients underwent endoscopic procedure. Biopsy samples were collected for urease test, histopathological and molecular exams. The DNA of theses samples was extracted for detection ofH. pyloriand analysis of the genes mentioned above. Patients with gastritis had a higher frequency ofH. pylori-positive samples.Results : H. pyloriwas detected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helicobacter pylori; Polymorphism; Interleukin.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200327
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Genetic characterisation of oyster populations along the north-eastern coast of Tunisia ArchiMer
Dridi, S.; Romdhane, M. S.; Heurtebise, Serge; El Cafsi, M.; Boudry, Pierre; Lapegue, Sylvie.
The taxonomy of oysters has been traditionally based on characteristics of the shell. More recently, the analysis of protein and DNA polymorphism has provided a means to overcome difficulties in distinguishing the different species of oysters based solely on shell morphology. In order to identify oysters of the Tunisian north-east coast, we sequenced a 16S rRNA mitochondrial fragment from 68 oysters sampled from the Bizert Lagoon and the Gulf of Hammamet in northern Tunisia. Comparison of oyster 16S rRNA sequences available in GenBank showed the presence of both Ostreola stentina and Crassostrea gigas in our samples, which could not be detected on the basis of shell morphology only. These data confirmed that C. gigas, a non-native species, is now...
Tipo: Text Palavras-chave: 16S rRNA; Crassostrea gigas; Genetics; Mediterranean Sea; Ostreola stentina; Polymorphism.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00000/11063/7820.pdf
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Genetic correlation between Prothrombin G20210A polymorphism and retinal vein occlusion risk BJMBR
Zou,Yuanyuan; Zhang,Xi; Zhang,Jingyi; Ji,Xiangning; Liu,Yuqing; Zhao,Shaozhen.
The aim of this study was to perform an updated meta-analysis to quantitatively investigate the association between G20210A polymorphism of Prothrombin gene and the risk of retinal vein occlusion (RVO), based on the available publications with inconsistent results. We utilized the Stata software to perform the heterogeneity test, association test, Begg's and Egger's tests, and sensitivity analysis. We searched three on-line databases (PubMed, Embase, and WOS) and obtained a total of 422 articles. Based on our selection criteria, 24 case-control studies were finally enrolled in this overall meta-analysis; a subgroup analysis by the factors ethnicity, control source, and RVO type was done. Through the association test of overall meta-analysis, we did not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prothrombin; Retinal vein occlusion; Polymorphism; Risk; Meta-analysis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400604
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Genetic diversity in a natural population of the halophytic legume Prosopis strombulifera revealed by AFLP fingerprinting Boletín de la Sociedad
Llanes,Analia; Bonercarrere,Victoria; Capdevielle,Fabian; Vidal,Sabina; Luna,Virginia.
Prosopis strombulifera (Lam.) Benth. is a spiny shrub with the maximum tolerance limits reported for halophytic plants. This species is frequently found in the salinized areas in south-western of Córdoba and San Luis provinces, Argentina. Little is known about the genetic diversity within this species in a native population. Genetic diversity in 60 plants of P. strombulifera in south-western San Luis was investigated using AFLP analysis. Polymorphism was found among the samples tested. Four combinations of primers led to the identification of an average of 250 polymorphic bands and the data were used for cluster analysis. P. strombulifera genotypes are clearly separated in subclusters and reflect the diversity within the collection area. This study is a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prosopis strombulifera; AFLP; Genetic diversity; Polymorphism.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722011000200010
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Genetic polymorphism and immune response to tuberculosis in indigenous populations: a brief review BJID
Longhi,Renata Maronna Praça; Zembrzuski,Verônica Marques; Basta,Paulo Cesar; Croda,Julio.
We systematically reviewed studies of the immune response to tuberculosis and the genetic polymorphisms associated with Th1-or Th2-mediated cytokine expression in indigenous populations. A bibliographic search was performed on the Medline and ISI databases and included studies published between January 1980 and October 2011. The search terms were tuberculosis, American Indians, Amerindian, indigenous, Indians, native people, aboriginal, immun*, host immune, immune response, cytokine*, polymorphism*, and gene. Regardless of their design, studies that evaluated immunoglobulin, cytokine levels and genetic polymorphisms that altered cytokine expression were included. Thirteen studies met the inclusion criteria. The majority of studies were performed in Latin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Immune response; Indigenous populations; Polymorphism.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300009
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