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| Registros recuperados: 216 | |
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| Saleh,Basel. |
| The goal of this study was to investigate the molecular characterization of A. macrostachyum genotypes grown in the Western coast of Syria using RAPD and ISSR techniques. PCR amplifications with 20 RAPD primers gave an average of 9.25 selected markers/ primer, with a maximum of 17 (OPG05) and a minimum of four (OPA02). Percentage of polymorphic bands ranged from 40 to 100% according to RAPD primers tested. Among the 185 selected bands, 160 (84.96%) were polymorphic. The amplification with seven ISSR primers generated 88 bands, and 80 (90.91%) were polymorphic. (CA)8RG and (AG)8GTG ISSR primers tested in this study yielded highly informative patterns. RAPD and ISSR fragment sizes ranged from 0.2-3 kb. Based on this study, the use of RAPD and ISSR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: A. macrostachyum; Genetic diversity; Genotype; ISSR; Polymorphism; RAPD. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500002 |
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| Song,Guo Qi; Li,Meng Jun; Xiao,Han; Wang,Xing Jun; Tang,Rong Hua; Xia,Han; Zhao,Chuan Zhi; Bi,Yu Ping. |
| Making use of the gene resources of wild type peanuts is a way to increase the genetic diversity of the cultivars. Marker assisted selection (MAS) could shorten the process of inter-specific hybridization and provide a possible way to remove the undesirable traits. However, the limited number of molecular markers available in peanut retarded its MAS process. We started a peanut ESTs (Expressed Sequence Tags) project aiming at cloning genes with agronomic importance and developing molecular markers. In this study we found 610 ESTs that contained one or more SSRs from 12,000 peanut ESTs. The most abundant SSRs in peanut are trinucleotides (66.3%) SSRs and followed by dinucleotide (28.8%) SSRs. AG/TC (10.7%) repeat was the most abundant and followed by CT/GA... |
| Tipo: Journal article |
Palavras-chave: Arachis hypogaea L.; Cultivars; EST-SSR; Polymorphism; Wild-type peanut. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300007 |
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| COUTINHO FILHO, J. L. V.; PERES, R. M.; JUSTO, C. L.; ALENCAR, M. M. de; RAZOOK, A. G.; SILVA, L. R. M. da; SIQUEIRA, P. A.. |
| O objetivo do trabalho foi avaliar uma possivel associação entre polimorfismo para cor da mufla e desempenho de tourinhos Canchim. O trabalho foi realizado em dois locais diferentes. Na Fazenda São Jorge (Cedral-SP), foram utilizados 51 aimais inteiros da raça Canchim com médias de 20 meses de idade e 327 kg de peso vivo ao inicio do período, e classificados em 3 grupos em relação a cor de mufla (escura, rosada e pigmentação intermediaria). O mesmo procedimento for feito para 105 animais que estavam participando da prova de Ganho de Peso na Estação Experimental de Zootecnia de Sertãozinho (SP), iniciando a prova com 210 dias e terminando com 378 dias de idade. Os resultados mostraram que não existiu associação significativa entre os grupos com diferentes... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Gado de Corte; Cor da mufla; Ganho de peso; Polimorfismo; Beef cattle; Gain of weight; Polymorphism. |
| Ano: 1996 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/43280 |
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| Tomasco,Ivanna; Wlasiuk,G.; Lessa,E.P.. |
| The allele frequencies of 10 microsatellite loci previously described for sheep as BM1314, BM6526, OarFCB128, OarHH64, OarCP20, OarHH47, OarFCB48, OarHH35, OarHH72 and BM2508 were estimated for the Uruguayan flocks. A representative sample of 101 individuals composed by the two predominant breeds (76% Corriedale and 24% Australian Merino) was used. The sample did not show a significant tendency towards substructuring, in spite of presenting some significantly different allele frequencies between races. The Corriedale sample presents three loci in which the presence of null alleles is possible. The markers were highly variable, showing between 7 and 15 alleles each. The Polymorphism Information Content Index ranged from 0.63 to 0.87 and the Exclusion... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sheep; Polymorphism; Microsatellites. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100008 |
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| Levi, A.; Wechter, W.P.; Harris, K.R.; Davis, A.R.; Fei, Z.; Giovannoni, J.J.; Trebitsh, T.; Salman, A.; Hernandez, A.; Thimmapuram, J.; Tadmor, Y.; Portnoy, V.; Katzir, N.. |
| Over 8,000 ESTs were generated for watermelon and were assembled into 4,700 EST-unigenes (http://www.icugi.org). Microarray and Real-Time PCR analyses were used to examine differential expression of 832 of these EST-unigenes in developing and ripening watermelon fruit. RNA was isolated from watermelon fruits(in three sequential stages of development and ripening) and from young leaves of field grown plants (during three consecutive years). The microarray analysis identified 335 EST-unigenes differentially expressed (by at least two-fold when compared to leaf) in watermelon fruit during the early, ripening, or mature stage. A considerable number of these watermelon ESTs are associated with vascular system differentiation and development, ethylene... |
| Tipo: Conference Paper |
Palavras-chave: Watermelon; Microarray; Polymorphism; PCR analysis; Expressed sequence tag; EST; Gene expression. |
| Ano: 2008 |
URL: http://hdl.handle.net/2174/216 |
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| Pérez Ruíz, Elizabeth. |
| El Factor 9 de Crecimiento y Diferenciación (GDF9) es miembro de la súperfamilia β de factores de crecimiento (TGFβ) y su expresión en el ovocito es esencial para el desarrollo y crecimiento folicular. Diferentes mutaciones en el gen GDF9 han sido asociadas con el incremento de tasa de ovulación y/o prolificidad en algunas razas de ovejas, por lo que el objetivo de este estudio fue la búsqueda de polimorfismos de una sola base (SNPs) en el gen GDF9, así como la asociación entre polimorfismos del gen GDF9 y el índice de prolificidad en ovejas de la raza Pelibuey. Se tomaron muestras sanguíneas de la vena yugular de 16 ovejas y fueron conservadas en papel FTA® (Whatman Mini Card). El exón dos del gen fue amplificado mediante la técnica de Polimerase Chain... |
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Palavras-chave: Análisis de secuencias; GDF9; Mutación; Polimorfismo; PCR; SNP; Sequence analysis; Mutation; Polymorphism; Maestría; Ganadería. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1712 |
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| Valenzuela,Carlos Y. |
| The Neutral Theory of Evolution (NTE) proposes mutation and random genetic drift as the most important evolutionary factors. The most conspicuous feature of evolution is the genomic stability during paleontological eras and lack of variation among taxa; 98% or more of nucleotide sites are monomorphic within a species. NTE explains this homology by random fixation of neutral bases and negative selection (purifying selection) that does not contribute either to evolution or polymorphisms. Purifying selection is insufficient to account for this evolutionary feature and the Nearly-Neutral Theory of Evolution (N-NTE) included negative selection with coefficients as low as mutation rate. These NTE and N-NTE propositions are thermodynamically (tendency to random... |
| Tipo: Journal article |
Palavras-chave: Fixation; Loss; Neutral evolution; Polymorphism; Randomness; Selectiveness. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200001 |
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| Hubácek,Jaroslav A.; Adámková,Věra; Šedová,Lenka; Olišarová,Věra; Adámek,Václav; Tóthová,Valérie. |
| Abstract Lactase non-persistence (leading to primary lactose intolerance) is a genetically dependent inability to digest lactose in adulthood. As part of the human adaptation to dairying, the human lactase LCT-13910C/T mutation (which propagates adult expression of lactase) developed, spread and participated in the adaptation to dairying. This variant is associated with lactase activity persistence, and its carriers are able to digest lactose. We compared the frequencies of lactase 13910C/T (rs4988235) genotypes in Czechs/Slavs (N = 288) and Czech Gypsies/Roma (N = 300), two ethnically different groups where this polymorphism has not yet been analysed. Allelic frequencies significantly differed between the populations (p < 0.0001). In Czechs/Slavs, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactose; Lactase persistence; Polymorphism; Czech/Slav; Czech Gypsy/Roma. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300450 |
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| Dridi, S.; Romdhane, M. S.; Heurtebise, Serge; El Cafsi, M.; Boudry, Pierre; Lapegue, Sylvie. |
| The taxonomy of oysters has been traditionally based on characteristics of the shell. More recently, the analysis of protein and DNA polymorphism has provided a means to overcome difficulties in distinguishing the different species of oysters based solely on shell morphology. In order to identify oysters of the Tunisian north-east coast, we sequenced a 16S rRNA mitochondrial fragment from 68 oysters sampled from the Bizert Lagoon and the Gulf of Hammamet in northern Tunisia. Comparison of oyster 16S rRNA sequences available in GenBank showed the presence of both Ostreola stentina and Crassostrea gigas in our samples, which could not be detected on the basis of shell morphology only. These data confirmed that C. gigas, a non-native species, is now... |
| Tipo: Text |
Palavras-chave: 16S rRNA; Crassostrea gigas; Genetics; Mediterranean Sea; Ostreola stentina; Polymorphism. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00000/11063/7820.pdf |
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| Zou,Yuanyuan; Zhang,Xi; Zhang,Jingyi; Ji,Xiangning; Liu,Yuqing; Zhao,Shaozhen. |
| The aim of this study was to perform an updated meta-analysis to quantitatively investigate the association between G20210A polymorphism of Prothrombin gene and the risk of retinal vein occlusion (RVO), based on the available publications with inconsistent results. We utilized the Stata software to perform the heterogeneity test, association test, Begg's and Egger's tests, and sensitivity analysis. We searched three on-line databases (PubMed, Embase, and WOS) and obtained a total of 422 articles. Based on our selection criteria, 24 case-control studies were finally enrolled in this overall meta-analysis; a subgroup analysis by the factors ethnicity, control source, and RVO type was done. Through the association test of overall meta-analysis, we did not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prothrombin; Retinal vein occlusion; Polymorphism; Risk; Meta-analysis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400604 |
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| Longhi,Renata Maronna Praça; Zembrzuski,Verônica Marques; Basta,Paulo Cesar; Croda,Julio. |
| We systematically reviewed studies of the immune response to tuberculosis and the genetic polymorphisms associated with Th1-or Th2-mediated cytokine expression in indigenous populations. A bibliographic search was performed on the Medline and ISI databases and included studies published between January 1980 and October 2011. The search terms were tuberculosis, American Indians, Amerindian, indigenous, Indians, native people, aboriginal, immun*, host immune, immune response, cytokine*, polymorphism*, and gene. Regardless of their design, studies that evaluated immunoglobulin, cytokine levels and genetic polymorphisms that altered cytokine expression were included. Thirteen studies met the inclusion criteria. The majority of studies were performed in Latin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Immune response; Indigenous populations; Polymorphism. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300009 |
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| Registros recuperados: 216 | |
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