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Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa Plantas Medicinales
Cesty Borda-Yepez,Charlotte; Saenz Tejada,Erik C; Pereira Lima,Giuseppina Pace.
Este trabajo tuvo como objetivo estandarizar el protocolo de desinfección de semillas, germinación y obtención de plántulas in vitro por medio de callos de Pothomorphe umbellata (L.) Miq. Las semillas germinadas de P. umbellata fueron inoculadas en diferentes concentraciones y combinaciones de BAP (benzilaminopurina) y NAA (ácido naftalenacético) para estimular la producción de callos. Después de 60 días de cultivo, los callos que contenían algunas brotaciones, fueron llevados para medio de organogénesis (GA3 0,1 mg.L-1, BAP 0,5 mg.L-1) por un período de 40 días, para después ser transferido para medio de desarrollo de las plántulas. Finalmente, las plántulas fueron climatizadas y presentaron buen índice de sobrevivencia.
Tipo: Journal article Palavras-chave: Pothomorphe umbellata; Reguladores vegetales; Plantas medicinales.
Ano: 2007 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962007000400006
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Dose-dependent in vitro inhibition of rabbit corneal matrix metalloproteinases by an extract of Pothomorphe umbellata after alkali injury BJMBR
Barros,L.F.M.; Barros,P.S.M.; Röpke,C.D.; Silva,V.V.; Sawada,T.C.H.; Barros,S.B.M.; Belfort Jr.,R..
The in vitro ability of Pothomorphe umbellata ethanolic crude extract to inhibit matrix metalloproteinase (MMP) in normal cornea and in cornea after alkali injury was demonstrated. Corneas of albino rabbits were injured with 1 N NaOH for 20 s. After 48 h the corneas were excised, homogenized and analyzed for MMP-9 (92 kDa), pro-MMP-2 (72 kDa) and MMP-2 (67 kDa) activity by gelatin zymography. The activity was also measured in untreated corneas. After electrophoresis of 20 µg protein, gels were incubated with 50, 100, or 250 µg/mL lyophilized hydroethanolic (1:1) root crude extract of P. umbellata standardized for 4-nerolidylcatechol (7.09%). The activity of the enzymes was compared with that of untreated gel. At 48 h after injury, the activity of all MMPs...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cornea; Alkali injury; Metalloproteinases; Pothomorphe umbellata.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800014
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Cytotoxicity and antitumoral activity of dichloromethane extract and its fractions from Pothomorphe umbellata BJMBR
Sacoman,J.L.; Monteiro,K.M.; Possenti,A.; Figueira,G.M.; Foglio,M.A.; Carvalho,J.E..
The cytotoxicity of the dichloromethane crude extract (DCE), obtained from the aerial parts of Pothomorphe umbellata (L.) Miq (Piperaceae), was evaluated against nine human cancer cell lines (MCF-7, NCI-ADR/RES, OVCAR-3, PC-3, HT-29, NCI-H460, 786-O, UACC-62, K-562). The DCE presented antiproliferative activity with good potency against all cell lines at low concentrations (between 4.0 and 9.5 µg/mL) and with selectivity (1.55 µg/mL) for the leukemia cell line (K-652). DCE (100, 200, 300 and 400 mg/kg, ip) was also evaluated in the Ehrlich ascites tumor model. Both the survival number and the life span of the animals that died increased by at least 45 and 50%, respectively (8 animals per group), demonstrating P. umbellata extract potential anticancer...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Pothomorphe umbellata; Piperaceae; Antitumor activity; Ehrlich ascites tumor.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500010
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