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A bioassay method for black flies (Diptera: Simuliidae) using larvicides Neotropical Entomology
Araújo-Coutinho,Carlos J.P.C.; Figueiró,Ronaldo; Viviani,Andréia P.; Nascimento,Érika S.; Cavados,Clara F.G..
This note presents an alternative method for bioassays using black fly larvae, with which we intend to provide a simpler but effective tool for accessing data on larvicide efficacy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Efficacy; Technique.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300022
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A biocultural approach to the use of natural resources in Northeast Brazil: A socioeconomic perspective Acta Botanica
Silva,Nylber Augusto da; Alves,Ângelo Giuseppe Chaves; Albuquerque,Ulysses Paulino de; Ramos,Marcelo Alves.
ABSTRACT We employed a biocultural approach to understanding the dynamics of knowledge ans use of natural resources associated in immaterial aspects of culture. We investigated whether factors such as sex, income, age, religion, occupation and time of participation in cultural practice influence the richness of species known and used by members of Cavalo Marinho, expression of the popular culture of the Brazilian Northeast that brings together theater, music and dance. We recorded a total of 111 ethnospecies (95 plants and 16 animals), based on information obtained from 56 informants. .There was a predominance of native plants and domestic animals in the knowledge of the participants in this cultural expression, although effective use is restricted to few...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocultural approach; Biocultural heritage; Cultural practice; Intracultural variation; Knowledge and use; Natural resources.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000200315
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A biodegradation study of forest biomass by Aspergillus niger F7: correlation between enzymatic activity, hydrolytic percentage and biodegradation index BJM
Sharma,Nivedita; Kaushal,Richa; Gupta,Rakesh; Kumar,Sanjeev.
Aspergillus niger F7 isolated from soil was found to be the potent producer of cellulase and xylanase. The residue of forest species Toona ciliata, Celtris australis, Cedrus deodara and Pinus roxburghii was selected as substrate for biodegradation study due to its easy availability and wide use in industry. It was subjected to alkali (sodium hydroxide) treatment for enhancing its degradation. Biodegradation of forest waste by hydrolytic enzymes (cellulase and xylanase) secreted by A. niger under solid state fermentation (SSF) was explored. SSF of pretreated forest biomass was found to be superior over untreated forest biomass. Highest extracellular enzyme activity of 2201±23.91 U/g by A. niger was shown in pretreated C. australis wood resulting in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; SSF; Enzyme activity; Percent hydrolysis; Biodegradation index.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200006
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A biodistribution study of Hemiscorpius lepturus scorpion venom and available polyclonal antivenom in rats J. Venom. Anim. Toxins incl. Trop. Dis.
Seyedian,R; Jalali,A; Babaee,MH; Pipelzadeh,MH; Rezaee,S.
The purpose of the present study was to investigate the biodistribution profile of the venom of Hemiscorpius lepturus, the most dangerous scorpion in Iran. Blood and tissue samples were taken at various predetermined intervals during a 400-minute period for the venom and a 360-minute period for the antivenom in rats. The radio-iodination was carried out using the chloramine-T method. The results showed that the descending order of venom uptake was skin, kidneys and intestine, respectively. The descending order of polyclonal antivenom uptake was kidneys, intestine, heart and lungs. The calculated pharmacokinetic parameters of the venom were Telimination half-life = 521.5 ± 12.6 minutes; Vd/F (apparent volume of distribution) = 14.9 ± 3.3 mL; clearance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemiscorpius lepturus scorpion venom; Polyvalent antivenom; Pharmacokinetic parameters; Tissue distribution.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400005
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A biogeographical population genetics perspective of the colonization of cats in Latin America and temporal genetic changes in Brazilian cat populations Genet. Mol. Biol.
Ruiz-García,Manuel; Alvarez,Diana.
We used nine morphological genes to analyze cat populations from Mexico, the Dominican Republic, the Colombian, Brazilian and Peruvian Amazon, Bolivia and Brazil. Most populations were in Hardy-Weinberg equilibrium at the O locus. The highest allele frequencies so far detected at world level for alleles I (inhibitor) and L (long hair) were found at La Paz (Bolivia). The analyses revealed at least five cat gene pools in Latin America: These findings suggest that the current genetic distribution of cats in Latin America correlates with the colonization of the Americas during the XIV to XVIII centuries. Additionally, the cat populations of São Paulo, Rio de Janeiro and Manaus were sampled, to compare their 1996-2003 genetic profiles with those obtained in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Domestic cats; Latin American cats; Population genetics; Genetic changes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400026
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A biolistic process for in vitro gene transfer into chicken embryos BJMBR
Ribeiro,L.A.; Mariani,P.D.S.C.; Azevedo,J.L.; Rech,E.L.; Schmidt,G.S.; Coutinho,L.L..
Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions obtained for high embryo survival and high transformation efficiency were achieved with 800 psi helium gas pressure, 500 mmHg vacuum, gold particles, an 8 cm DNA-coated microparticle flying distance to the embryo and embryo placement 0.5 cm from the center of the particle dispersion cone. Under these conditions, transformation efficiency was 100%, survival rate 25% and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biolistic process; Chicken embryo; Gene transfer; SS-galactosidase; Green fluorescent protein; GFP.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900003
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A biological treatment technique for wool textile BABT
Xiao-Wei,Yu; Guan,Wen-Jun; Yong-Quan,Li; Ting-Jing,Guo; Ji-Dong,Zhou.
A biological treatment technique for wool textile was carried out by enzymes degradation coupled with H2O2 oxidation. The results demonstrated that the technique had ideal effects on wool textile such as better softness, plump and less loss of bursting stress. Because of mild reaction conditions, less textile damage and less environmental pollution, this technique for wool textile treatment could have promising prospect.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wool textile decrement; Oxidation pretreatment; Enzyme degradation; L86 enzymes.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600001
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A biomechanical study on proximal junctional kyphosis following long-segment posterior spinal fusion BJMBR
Zhu,Wen-Yi; Zang,Lei; Li,Jian; Guan,Li; Hai,Yong.
Posterior long-segment spinal fusion may lead to proximal junctional kyphosis (PJK). The present study sought to identify the appropriate fusion levels required in order to prevent PJK using finite element analysis. A finite element model was constructed based on the whole-spine computed tomography findings of a healthy adult. Nine commonly used posterior spinal fusion methods were selected. Stress on the annulus fibrosis fibers, the posterior ligamentous complex, and the vertebrae after various spinal fusions in the upright position were compared. This study was divided into two groups: non-fusion and fusion. In the former, the stress between the T10 and the upper thoracic vertebrae was higher. Comparing thoracic and lumbar segments in the fusion group,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adult spinal deformity; Proximal junctional kyphosis; Upper instrumented vertebra; Functional spinal unit; Finite element.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500604
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A Biosensor Using Poly(4-Aminophenol)/acetylcholinesterase modified graphite electrode for the detection of dichlorvos BABT
Melo,Edmar Isaías; Franco,Diego Leoni; Afonso,André Santiago; Rezende,Hélen Cristine; Brito-Madurro,Ana Graci; Madurro,João Marcos; Coelho,Nívia Maria Melo.
The properties of poly(4-aminophenol) modified graphite electrode as material for the immobilization of acetylcholinesterase were investigated by the Cyclic Voltammetry, Electrochemical Impedance Spectroscopy and Atomic Force Microscopy. The polymer was deposited on graphite electrode surface by the oxidation of 4-aminophenol and then acetylcholinesterase was immobilized on the surface of the electrode. The biosensor coupled in the continuous flow system was employed for the detection of dichlorvos. The detection and quantification limits were 0.8 and 2.4 μmol L-1 dichlorvos, respectively. Graphite electrodes modified with the poly(4-aminophenol) showed to be an efficient and promising material for immobilization of acetylcholinesterase enzyme. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Graphite; Poly(4-aminophenol); Biosensor; Dichlorvos.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600018
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A biotechnology perspective of fungal proteases BJM
Souza,Paula Monteiro de; Bittencourt,Mona Lisa de Assis; Caprara,Carolina Canielles; Freitas,Marcela de; Almeida,Renata Paula Coppini de; Silveira,Dâmaris; Fonseca,Yris Maria; Ferreira Filho,Edivaldo Ximenes; Pessoa Junior,Adalberto; Magalhães,Pérola Oliveira.
Proteases hydrolyze the peptide bonds of proteins into peptides and amino acids, being found in all living organisms, and are essential for cell growth and differentiation. Proteolytic enzymes have potential application in a wide number of industrial processes such as food, laundry detergent and pharmaceutical. Proteases from microbial sources have dominated applications in industrial sectors. Fungal proteases are used for hydrolyzing protein and other components of soy beans and wheat in soy sauce production. Proteases can be produced in large quantities in a short time by established methods of fermentation. The parameters such as variation in C/N ratio, presence of some sugars, besides several other physical factors are important in the development of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteases; Enzyme production; Fungal protease; Industrial application.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200337
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A bipartição como alternativa para melhorar os índices de gestação na transferência de embriões eqüinos Ciência Rural
Silveira,Leonardo Luiz da; Souza,Regivaldo Vieira de; Melo,Natalie Soter Sousa; Pezzini,Tomaz Gelson; McManus,Concepta; Rumpf,Rodolfo.
A transferência de embriões (TE) já vem sendo utilizada em eqüinos há pelo menos duas décadas, sempre a partir de ovulação simples. Para aumentar a eficiência reprodutiva, este estudo avaliou a bipartição embrionária como uma alternativa para melhorar os índices de gestação na transferência de embriões em eqüinos. Foram utilizadas 21 éguas de diferentes padrões raciais, com idade variando entre 4 e 15 anos de idade, pesando entre 270 a 480kg. A partir da identificação do cio (rufiação), os animais foram monitorados através de exames ultra-sonográficos trans-retais até o momento da ovulação, sendo as receptoras, uma vez ao dia e as doadoras três vezes ao dia. As receptoras utilizadas ovularam um dia antes ou até três dias depois das doadoras. As doadoras...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gestação; Hemi-embriões; Índice de produção; Prenhez.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000200026
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A bovine herpesvirus 5 recombinant defective in the thymidine kinase (TK) gene and a double mutant lacking TK and the glycoprotein E gene are fully attenuated for rabbits BJMBR
Silva,S.C.; Brum,M.C.S.; Weiblen,R.; Flores,E.F.; Chowdhury,S.I..
Bovine herpesvirus 5 (BoHV-5), the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in South America and several efforts have been made to produce safer and more effective vaccines. In the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent Brazilian BoHV-5 strain (SV507/99). The recombinants are defective in glycoprotein E (BoHV-5gEΔ), thymidine kinase (BoHV-5TKΔ) and both proteins (BoHV-5gEΔTKΔ). Rabbits inoculated with the parental virus (N = 8) developed neurological disease and died or were euthanized in extremis between days 7 and 13 post-infection (pi). Infectivity was detected in several areas of their brains. Three of 8 rabbits inoculated with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BoHV-5; GE and TK deletion mutants; Pathogenesis; Virulence; Vaccine candidate.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200005
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A bovine teat papilloma specimen harboring Deltapapillomavirus (BPV-1) and Xipapillomavirus (BPV-6) representatives BABT
Claus,Marlise Pompeo; Lunardi,Michele; Alfieri,Amauri Alcindo; Otonel,Rodrigo Alejandro Arellano; Ferracin,Lara Munique; Fungaro,Maria Helena Pelegrinelli; Alfieri,Alice Fernandes.
The common occurrence of multiple papillomavirus infections has been shown in several studies involving the human host. However, investigations with the aim of identifying mixed papillomavirus infections in cattle have been conducted only recently. In the current work we describe a co-infection with two different bovine papillomavirus (BPV) types that was identified in a bovine teat papilloma. The skin wart was obtained from a cow belonging to a Brazilian beef herd. A PCR assay was carried out with the FAP primer pair, which amplifies a partial segment of the L1 gene (approximately 478 bp), and the amplicon was submitted to direct sequencing. Because nucleotide sequences with satisfactory quality scores were not obtained, the amplicon was cloned and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Cutaneous papillomatosis; BPV; Co-infection.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700012
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A box-model of metal flows through the Scheldt estuary (1981-1983 and 1992-1995) OMA
Baeyens, W.F.J.; Monteny, F.; Van Ryssen, R.; Leermakers, M..
The behaviour of Cu, Zn, Cd, and Pb in the Scheldt estuary was investigated in the periods 1981 to 1993 and 1995. The Hg behaviour was studied between 1992 and 1994. A box model was used to establish the relationship between the trace metal inputs and the fate of these pollutants in the Scheldt estuary. Annual budgets were calculated for particulate and dissolved trace metals. For Hg, seasonal budgets of the various species (inorganic Hg, methylmercury, Hg°) could also be calculated. These budgets allow an evaluation of the consistency of each individual flux or process, such as the inputs of the various metals into the North Sea, the sedimentation flux of particulate metals in the area of high turbidity, the evasional fluxes and the formation rates of...
Tipo: Info:eu-repo/semantics/article
Ano: 1998 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=7059
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A bradykinin-potentiating peptide (BPP-10c) from Bothrops jararaca induces changes in seminiferous tubules J. Venom. Anim. Toxins incl. Trop. Dis.
Gilio,Joyce M; Portaro,Fernanda CV; Borella,Maria I; Lameu,Claudiana; Camargo,Antonio CM; Alberto-Silva,Carlos.
Background The testis-specific isoform of angiotensin-converting enzyme (tACE) is exclusively expressed in germ cells during spermatogenesis. Although the exact role of tACE in male fertility is unknown, it clearly plays a critical function in spermatogenesis. The dipeptidase domain of tACE is identical to the C-terminal catalytic domain of somatic ACE (sACE). Bradykinin potentiating peptides (BPPs) from snake venoms are the first natural sACE inhibitors described and their structure-activity relationship studies were the basis for the development of antihypertensive drugs such as captopril. In recent years, it has been showed that a number of BPPs - including BPP-10c - are able to distinguish between the N- and C-active sites of sACE, what is not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bradykinin-potentiating peptide; Snake venom; Angiotensin-converting enzyme; Testis; Seminiferous epithelium.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100316
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A brain microdialysis study on 5-HT release in freely moving rat lines selectively bred for differential 5-HT1A receptor function BJMBR
Gonzalez,L.E.; Parada,M.A.; Tucci,S.; Teneud,L.; Overstreet,D.H.; Hernandez,L..
Breeding for high and low hypothermic responses to systemic administration of a serotonin1A (5-HT1A) receptor agonist (8-hydroxy-2-(di-n-propylamino)tetralin, 8-OH-DPAT) has resulted in high DPAT-sensitive (HDS) and low DPAT-sensitive (LDS) lines of rats, respectively. These lines also differ in several behavioral measures associated with stress. In the present microdialysis study we observed that basal 5-HT concentrations in the prefrontal cortex and dorsal hippocampus did not differ significantly between HDS and LDS rats. Thus, behavioral differences between the HDS and LDS lines might not be attributed to differences in basal 5-HT release. However, both lines had lower basal levels of 5-HT release than their randomly bred control group (random...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brain microdialysis; Serotonin; 5-HT1A rat lines; Fenfluramine; 8-OH-DPAT.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200014
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A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae) BJM
Gitahy,Patrícia de Medeiros; Souza,Marlene Teixeira de; Monnerat,Rose Gomes; Arrigoni,Enrico de Beni; Baldani,José Ivo.
The control of the major sugarcane pest, Diatraea saccharalis, is limited by the stem location of the caterpillar. As part of a long-term project towards the development of an alternative and efficient delivery system of Cry proteins to control the sugarcane borer, the current work describes the selection and characterization of a Brazilian B. thuringiensis strain with prominent activity towards D. saccharalis. Strain S76 was eleven-fold more active than the HD-1 Lepidoptera-standard strain, as estimated by the LC50 of 13.06 µg/L and 143.88 µg/L, respectively. We observed bipiramidal and cuboidal crystals similar to those found in other B. thuringiensis strains with entomopathogenic activity against Lepidoptera and Diptera. In addition, smaller and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Bacillus thuringiensis kurstaki; Cry toxins; Spherical crystal; Diatraea saccharalis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300028
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A Brazilian family with hereditary inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia BJMBR
Fanganiello,R.D.; Kimonis,V.E.; Côrte,C.C.; Nitrini,R.; Passos-Bueno,M.R..
Inclusion body myopathy associated with Paget disease and frontotemporal dementia (IBMPFD) is a progressive and usually misdiagnosed autosomal dominant disorder. It is clinically characterized by a triad of features: proximal and distal myopathy, early onset Paget disease of bone (PDB), and frontotemporal dementia (FTD). It is caused by missense mutations in the valosin-containing protein (VCP) gene. We describe here the clinical and molecular findings of the first Brazilian family identified with IBMPFD. Progressive myopathy affecting the limb girdles was detected by clinical examination followed by muscle biopsy and creatine kinase measurement. PDB was suggested after anatomopathological bone examination and FTD was diagnosed by clinical,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Frontotemporal dementia; VCP gene mutations; Myopathy; Paget disease of bone.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400016
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A brief note on the sleeping habits of the giant anteater - Myrmecophaga tridactyla Linnaeus (Xenarthra, Myrmecophagidae) Zoologia
Medri,Ísis Meri; Mourão,Guilherme.
The scientific literature on giant anteaters states that the animal sleeps with its tail folded over its body to conserve body temperature. However, observations of this species in natural habitats indicate variations in this behavior, depending on the ambient temperature.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ambient temperature; Body temperature; Pantanal.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752005000400061
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A brief report on some health aspects of rats fed with crescent levels of recombinant chagasin, a potential plant defense protein Anais da ABC (AABC)
Oliveira Neto,Osmundo B.; Farias,Davi F.; Vasconcelos,Ilka M.; Paes,Norma S.; Monteiro,Ana C. S.; Silva,Maria C. M. da; Guimarães,Luciane M.; Carvalho,Ana F.U.; Grossi-de-Sá,Maria F..
Chagasin may be considered a potential plant-incorporated protectant (PIP) protein due to its deleterious effects on insect pests. However, extensive safety studies with PIP's are necessary before introducing them into the target plant. Thus, a short-term feeding trial in rats with high doses of r-chagasin was conducted to provide evidences about its safety. Three test diets containing casein + r-chagasin (0.25, 0.5 and 1% of total protein) were offered to rats (10 days). The test diets did not show adverse effects upon the development, organ weight, hematological parameters and serum protein profiles of rats, providing preliminary information on the safety of r-chagasin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chagasin; Recombinant protein; Hazard potential; Short-term rat feeding trial.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100019
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