SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

SANTOS, M. de L.; ALVES, G. S. C.; COTTA, M. G.; FONSECA, F. C. A.; TOGAWA, R. C.; COSTA, M. M. do C.; ALVES, A. A.; MILLER, R. N. G.; SOUZA JUNIOR, M. T..

bitstream/item/171242/1/Resumo50CBFito-0637.pdf

Análises da expressão gênica por PCR quantitativo em tempo real (QPCR) de genes candidatos para a tolerância à seca em cafeeiro.

Palavras-chave: E guineensis; E oleifera; NBS-LRRs; Biotic stress.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085646

Construction and characterization of a BAC library from the Coffea arabica genotype Timor Hybrid CIFC 832/2.

VINECKY, F.; ALVES, G. S. C.; VIEIRA, N. G.; FREIRE, L. P.; HEIMBECK, I. G. R.; FERRÃO, R. G.; FONSECA, A. F. A. da; FERRAO, M. A. G.; SILVA, V. A.; MARRACCINI, P.; ANDRADE, A. C..

A seca é uma das principais limitações climáticas à produção do cafeeiro. A importância dessa limitação deve aumentar, em função das mudanças reconhecidas no clima global e, também, porque a cafeicultura vem sendo expandida para regiões marginais onde a baixa pluviosidade e temperaturas desfavoráveis se constituem grandes limitações à produção do café. A seca induz diversas respostas fisiológicas e moleculares nas plantas, incluindo alterações da expressão gênica, visando atingir ajuste osmótico, a indução de reparadores de sistemas moleculares e a expressão de diversas proteínas protetoras. Existem diversas formas de se avaliar a expressão gênica em plantas submetidas ao estresse hídrico. Uma técnica amplamente utilizada nos últimos anos, para a análise...

Tipo: Artigo em anais de congresso (ALICE)

Palavras-chave: Coffea canephora; Expressão gênica; Gene candidato; Seca; Coffea canephora; Candidate genes; Drought; Gene expression.

Ano: 2011

URL: http://www.alice.cnptia.embrapa.br/handle/doc/903864

CACAO, S. M. B.; SILVA, N. V.; DOMINGUES, D. S.; VIEIRA, L. G. E.; DINIZ, L. E. C.; VINECKY, F.; ALVES, G. S. C.; ANDRADE, A. C.; CARPENTIERI PIPOLO, V.; PEREIRA, L. F. P..

bitstream/item/179851/1/Cacao2013-Article-ConstructionAndCharacterizatio.pdf

Controlled irrigation and nitrogen, phosphorous and potassium fertilization affect the biochemical composition and quality of Arabica coffee beans.

Palavras-chave: Genética de planta; Marcador; Coffee; Genetic; Café; Genética.

Ano: 2013

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/968360

VINECKY, F.; DAVRIEUX, F.; MERA, A. C.; ALVES, G. S. C.; LAVAGNINI, G.; LEROY, T.; BONNOT, F.; ROCHA, O. C.; BARTHOLO, G. F.; GUERRA, A. F.; RODRIGUES, G. C.; MARRACCINI, P.; ANDRADE, A. C..

Controlled irrigation during the dry period associated with adequate nitrogen (N), phosphorous (P) and potassium (K) fertilization led to the accumulation of biochemical compounds in coffee beans considered as positive precursors of beverage quality. Adult plants of coffee (Coffea arabica ?Rubi?) were cultivated using different water regimes (WR) and fertilization conditions under the dry climate of the Brazilian Cerrado. Coffee-bean physical characteristics were evaluated as well as biochemical composition by near-infrared analysis. The K treatment mostly affected bean biochemistry, lipid and chlorogenic acid (CGA) contents, which increased with increasing amounts of fertilizer. Caffeine contents increased with higher amounts of N, but no significant...

Controlled irrigation and nitrogen, phosphorous and potassium fertilization affect the biochemical composition and quality of Arabica coffee beans.

Palavras-chave: Sistema de irrigação; Fertilidade do Solo; Fertilizante Nitrogenado; Fertilizante Potássico; Fertilizante Fosfatado; Coffea Arabica.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1065059

VINECKY, F.; DAVRIEUX, F.; MERA, A. C.; ALVES, G. S. C.; LAVAGNINI, G.; LEROY, T.; BONNOT, F.; ROCHA, O. C.; BARTHOLO, G. F.; GUERRA, A. F.; RODRIGUES, G. C.; MARRACCNINI, P.; ANDRADE, A. C..

Controlled irrigation during the dry period associated with adequate nitrogen (N), phosphorous (P) and potassium (K) fertilization led to the accumulation of biochemical compounds in coffee beans considered as positive pre-cursors of beverage quality. Adult plants of coffee (Coffea arabica?Rubi?) were cultivated using different water regimes (WR) and fertilization conditions under the dry climate of the Brazilian Cerrado. Coffee-bean physical characteristics were evaluated as well as biochemical composition by near-infrared analysis. The K treatment mostly affected bean biochemistry, lipid and chlorogenic acid (CGA) contents, which increased with increasing amounts of fertilizer. Caffeine contents increased with higher amounts of N, but no significant...

Differential fine-tuning of gene expression regulation in coffee leaves by CcDREB1D promoter haplotypes under water deficit.

Palavras-chave: Controlled irrigation; Caffeine.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1066248

ALVES, G. S. C.; TORRES, L. F.; DÉCHAMP, E.; BREITLER, J-C.; JOËT, T.; GATINEAU, F.; ANDRADE, A. C.; BERTRAND, B.; MARRACCINI, P.; ETIENNE, H..

Despite the importance of the DREB1D gene (also known as CBF4) in plant responses to water deficit and cold stress, studies analysing its regulation by transgenic approaches are lacking. In the current work, a functional study of three CcDREB1D promoter haplotypes (named HP15, HP16 and HP17) isolated from drought-tolerant and droughtsensitive clones of Coffea canephora was carried out in plants of C. arabica stably transformed by Agrobacterium tumefaciens by analysing their ability to regulate the expression of the uidA reporter gene in response to water deficit mimicked by polyethylene glycol (−2.0 MPa) and low relative humidity treatments.

Expressão diferencial de genes em Pleurotus pulmonarius associados a degradação enzimática e destoxificação da torta de pinhão-manso.

Palavras-chave: Coffe; Water deficit; Genetic transformation; Haplotypes; Reporter gene.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1084513

GOMES, T. G.; ALVES, G. S. C.; HADI, S. I. I. A.; COSTA, M. M. do C.; MENDONCA, S.; MILLER, R. N. G.; SIQUEIRA, F. G. de.

bitstream/item/184260/1/EnPI-2018-8.pdf

Expression analysis ofdrebsubfamily genes in leaves and roots of Coffea canephora Conilon subjected to Drought.

Palavras-chave: Transcritoma; NGS; Pré-tratamento biológico; Macrofungos; Jatropha Curcas.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1097269

REICHEL, T.; ALVES, G. S. C.; AQUINO, S. O.; CARNEIRO, F.; ANDRADE, A. C.; MARRACCINI, P..

2016

Functional analysis of different promoter haplotypes of the coffee (Coffea Canephora) CcDREB1D gene through genetic transformation of Nicotiana tabacum.

Palavras-chave: Coffea canephora; Temperature variations.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1066329

AQUINO, S. O.; CARNEIRO, FERNANDA A; REGO, E. C. S.; ALVES, G. S. C.; MARRACCINI, P.; ANDRADE, A. C..

2016

Gene expression analysis in Musa acuminata during compatible interactions with Meloidogyne incognita.

Palavras-chave: Coffea canephora; Conilon; DREB genes.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1066317

CASTAÑEDA, N. E. N.; ALVES, G. S. C.; ALMEIDA, R. M.; AMORIM, E. P.; FERREIRA, C. F.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SANTOS, J. R. P.; CARES, J. E.; MILLER, R. N. G..

Background and Aims: Endoparasitic root-knot nematodes (RKNs) ( Meloidogyne spp.) cause considerable losses in banana ( Musa spp.), with Meloidogyne incognita a predominant species in Cavendish sub-group bananas. This study investigates the root transcriptome in Musa acuminata genotypes 4297-06 (AA) and Cavendish Grande Naine (CAV; AAA) during early compatible interactions with M. incognita . Methods: Roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological cell transformation. RNA samples were extracted 3, 7 and 10 days after inoculation (DAI) with nematode J2 juveniles, and cDNA libraries were sequenced using lllumina HiSeq technology. Sequences were mapped to the M. acuminata ssp....

Influence of abiotic stresses in phenotypic expression of transgenic plants of Coffea Arabica under action CcDREB1D promoter.

Palavras-chave: Musa acuminata; Meloidogyne incognita; Root-knot nematode; Biotic stress; Transcriptome; Monocotyledons.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076414

Molecular responses of coffee plants to drought stress.

TORRES, L. F.; DECHAMP, E.; ALVES, G. S. C.; DINIZ, L. E. C.; PAIVA, L. V; BREITLER, J-C.; ANDRADE, A. C.; MARRACINI, P.; ETIENNE, HERVE.

2016

Tipo: Artigo em anais de congresso (ALICE)

Palavras-chave: DREB; Abiotic stress; Biotic stress.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1066290

MARRACCINI, P.; VIEIRA, N. G.; DUARTE, K. E.; AQUINO, S. O.; CARNEIRO, F. A.; COSTA, T. S.; SUJII, P. S.; VINECKY, F.; ALEKCEVETCH, J. C.; ALVES, G. S. C.; LEROY, T.; BELLIS, F. de; FERRÃO, M. A. G.; DAMATTA, F. M.; POT, D.; SILVA, V. A.; RODRIGUES, G. C.; ANDRADE, A. C..

With the aim to investigate the molecular mechanisms underlying drought tolerance in coffee plants of C. canephora and C. arabica, qPCR experiments identified more than 80 candidate genes (CGs) presenting differential gene expression between drought-tolerant and drought-susceptible clones/cultivars cultivated under different (with or without) irrigation conditions.

RBCS1 expression in coffee: coffea orthologs, coffea arabica homeologs, and expression variability between genotypes and under drought stress.

Palavras-chave: Tolerância à seca; Café; Water stress; Coffea; Drought tolerance.

Ano: 2013

URL: http://www.alice.cnptia.embrapa.br/handle/doc/979069

MARRACCINI, P.; FREIRE, L. P.; ALVES, G. S. C.; VIEIRA, N. G.; VINECKY, F.; ELBELT, S.; RAMOS, H. J. O.; MONTAGNON, C.; VIEIRA, L. G. E.; LEROY, T.; POT, D.; SILVA, V. A.; RODRIGUES, G. C.; ANDRADE, A. C..

Background: In higher plants, the inhibition of photosynthetic capacity under drought is attributable to stomatal and non-stomatal (i.e., photochemical and biochemical) effects. In particular, a disruption of photosynthetic metabolism and Rubisco regulation can be observed. Several studies reported reduced expression of the RBCS genes, which encode the Rubisco small subunit, under water stress. Results: Expression of the RBCS1 gene was analysed in the allopolyploid context of C. arabica, which originates from a natural cross between the C. canephora and C. eugenioides species. Our study revealed the existence of two homeologous RBCS1 genes in C. arabica: one carried by the C. canephora sub-genome (called CaCc) and the other carried by the C. eugenioides...

RNA-Seq em Coffea arabica: genes candidatos em condições de estresse abiótico.

Palavras-chave: Café; Expressão gênica; Sequência genética; Genética molecular; Coffea arabica; Coffea canephora; Gene expression; Molecular genetics.

Ano: 2011

URL: http://www.alice.cnptia.embrapa.br/handle/doc/905604

TORRES, L. F.; DÉCHAMP, E.; ALVES, G. S. C.; MARRACCINI, P.; ETIENNE, H.; ANDRADE, A. C..

A crescente pesquisa na área de expressão gênica em busca de genes candidatos associados às respostas aos estresses abióticos têm possibilitado estudar o transcriptoma e identificar genes responsivos a esses estresses. O principal objetivo deste trabalho foi obter uma visão geral dos genes ativos em folhas de café arábica variedade Caturra quando submetido a vários estresses abióticos e analisar estes genes identificados in silico por meio da técnica de RT-qPCR.

Transcriptome analysis in Coffea arabica, under several abiotic stresses, reveals differentially expressed genes in leaves.

Palavras-chave: Sequenciamento por síntese (SBS); Experimento RT-qPCR; Gene; Cafeeiro; Coffea arabica.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1084690

TORRES, L. F.; DÉCHAMP, E.; ALVES, G. S. C.; MARRACCINI, P.; ETIENNE, H.; ANDRADE, A. C..

In recent years, Coffea ssp. has become subject of increasing research in gene expression analysis, in the quest to find genetic factors associated to abiotic stress responses with special attention to transcription factors such as the DREB family. This focus was due to their involvement in the regulation of many stress-related genes that play an important role in cascading a response to an environmental stimuli. RNA-seq analysis, creates the possibility to s study the transcriptome and to identify differentially expressed genes upon an abiotic stress or any important agronomic trait. The main objectives of this work were to obtain an overview of the transcriptionally active genes in Arabica coffee leaves when subjected to several abiotic stresses and to...