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Registros recuperados: 35 | |
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ANDREOTE, F. D.; TEIXEIRA, M. A.; DIAS, A. C. F.; VIEIRA, R. F.. |
O objetivo desse estudo foi avaliar o efeito do cultivo orgânico certificado e do cultivo convencional do morangueiro na diversidade de Proteobacterias e Actinobacterias do solo por meio da utilização da técnica independente de cultivo de gel de eletroforese em gradiente desnaturante (DGGE). Os solos destes dois sistemas de cultivo foram coletados em uma área produtora de morango nas profundidades de 0-10 e 10-20 cm. As alterações nas comunidades bacterianas foram observadas para as Proteobacterias (Alfa e Beta), que se diferenciaram na análise dos perfis de DGGE por similaridade e análise de componentes principais (PCA). A comunidade de Actinobacterias mostrou-se resiliente, não sendo alterada devido à alteração da metodologia de cultivo. Desta maneira,... |
Tipo: Separatas |
Palavras-chave: Morango; Microbiologia do Solo.. |
Ano: 2008 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/16110 |
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SANTOS, S. N.; KAVAMURA, V.; CASTANHA, R. F.; ANDREOTE, F. D.; CARVALHO, J. E.; QUEIROZ, S. C. do N. de; MELO, I. S. de. |
This investigation describes the anticancer, antioxidant and antimicrobial properties of the extracts of Combretum rupicola, a native plant from Northeast of Brazil. Methanolic, ethyl acetate and chloroform extracts from leaves of C. rupicola were evaluated in relation to their potential in inhibiting the cell growth and cytotoxic properties against nine human cancer cell lines (MCF-7, NCI-ADR/RES, OVCAR-3, PC-3, HT-29, NCIH460, 786-O, UACC-62, K-562). In addition, antioxidant activity of these extracts was measured using DPPH as radical scavenging assay and the ntimicrobial activities against nine pathogens were tested by agar diffusion method. Preliminary results showed that the this plant demonstrates to have moderate activity against bacteria but, on... |
Tipo: Separatas |
Palavras-chave: Combretum rupicola; Planta medicinal; Extrato vegetal; Plant extracts; Medicinal plants; Antioxidant activity; Antineoplastic agents. |
Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/950033 |
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SOARES JÚNIOR, F. L.; ANDREOTE, F. D.; SILVA, J. L. da; QUEIROZ, J. F. de; MELO, I. S. de. |
A carcinicultura é uma atividade comercial crescente em diversos países tropicais. No Brasil, esta atividade tem se intensificado no litoral do NE. Existem diversas técnicas para a criação de camarão, porém muitas dessas têm causado impactos ambientais nos ecossistemas próximos às fazendas de criação, como exemplo em áreas de manguezal. O presente trabalho tem por finalidade o isolamento e avaliação da resistência de bactérias presentes na água de cultivo de camarão, avaliadas em seis pontos ao longo de um viveiro de criação. A comunidade bacteriana variou em sua densidade entre os pontos amostrados, sendo que dentro do viveiro a densidade bacteriana foi menor. No teste de resistência aos antibióticos, o crescimento de alguns isolados na presença de... |
Tipo: Separatas |
Palavras-chave: Carcinicultura.. |
Ano: 2008 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/15750 |
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ARAÚJO, V. L. V. P. de; LIRA JUNIOR, M. A.; SOUZA JÚNIOR, V. S. de; ARAUJO FILHO, J. C. de; FRACETTO, F. J. C.; ANDREOTE, F. D.; PEREIRA, A. P. de A.; MENDES JÚNIOR, J. P.; BARROS, F. M. do R.; FRACETTO, G. G. M.. |
World climate change has triggered soil water stress and imposed limitations on agricultural production. Plant growth-promoting bacteria (PGPBs) have been an efficient strategy to improve the biological supply and growth of plants under distinct abiotic stress conditions. We hypothesized that the soils from a temporary pond may harbor PGPBs with potential strains which increase maize tolerance to water deficit. We studied rhizosphere and bulk soil of Mimosa bimucronata in a temporary pond from semiarid Northeast Brazil to access strains with characteristics to promote plant growth and mitigate abiotic stress for maize crop. We isolated 355 bacterial isolates, from which 96 were selected based on the morphophysiological characterization to assess IAA... |
Tipo: Artigo de periódico |
Palavras-chave: PGPBs; Zonas úmidas; Semiárido; Estresse hídrico; Mimosa Bimucronata; Zea Mays; Wetlands; Semiarid zones; Water stress. |
Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125023 |
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PYLRO, V. S.; ROESCH, L. F. W.; ORTEGA, J. M.; AMARAL, A. M. do; TÓTOLA, M. R.; HIRSCH, P. R.; ROSADO, A. S.; GÓES-NETO, A.; SILVA, A. L. da C.; ROSA, C. A.; MORAIS, D. K.; ANDREOTE, F. D.; DUARTE, G. F.; MELO, I. S. de; SELDIN, L.; LAMBAIS, M. R.; HUNGRIA, M.; PEIXOTO, R. S.; KRUGER, R. H.; TSAI, S. M.; AZEVEDO, V.. |
The Brazilian Microbiome Project (BMP) aims to assemble a Brazilian Metagenomic Consortium/Database. At present, many metagenomic projects underway in Brazil are widely known. Our goal in this initiative is to co-ordinate and standardize these together with new projects to come. It is estimated that Brazil hosts approximately 20 % of the entire world's macroorganism biological diversity. It is 1 of the 17 countries that share nearly 70 % of the world's catalogued animal and plant species, and is recognized as one of the most megadiverse countries. At the end of 2012, Brazil has joined GBIF (Global Biodiversity Information Facility), as associated member, to improve the access to the Brazilian biodiversity data in a free and open way. This was an important... |
Tipo: Separatas |
Palavras-chave: Diversidade microbiana; Biodiversidade; Microrganismo; Biodiversity. |
Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1000621 |
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DIAS, A. C. F.; ANDREOTE, F. D.; DINI-ANDREOTE, F.; LACAVA, P. T.; SÁ, A. L. B.; MELO, I. S. de; AZEVEDO, J. L.; ARAUJO, W. L.. |
Mangrove ecosystems are environments subject to substantial degradation by anthropogenic activities. Its location, in coastal area, interfacing the continents and the oceans makes it substantially important in the prospection for biotechnological applications. In this study, we assessed the diversity of culturable bacteria present over the seasons at two depths (0-10 and 30-40 cm) in a mangrove sediment and in a transect area from the land to the sea. In total, 238 bacteria were isolated, characterized by Amplified Ribosomal DNA Restriction Analysis (ARDRA) and further identified, by Fatty Acid Methyl Esther (FAME-MIDI), into the orders of Vibrionales, Actinomycetales and Bacillales. Also the ability of the isolates in producing economically important... |
Tipo: Relatórios técnicos |
Palavras-chave: Biotecnologia; Mangue.. |
Ano: 2009 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/577022 |
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MARCON, J.; TAKETANI, R. G.; DINI-ANDREOTE, F.; MAZZERO, G. I.; SOARES JUNIOR, F. L.; MELO, I. S. de; AZEVEDO, J. L.; ANDREOTE, F. D.. |
Here, we report the draft genome sequence and the automatic annotation of Bacillus thuringiensis strain BrMgv02-JM63. This genome comprises a set of genes involved in the metabolism of chitin and N-acetylglucosamine utilization, thus suggesting the possible role of this strain in the cycling of organic matter in mangrove soils. |
Tipo: Separatas |
Palavras-chave: Bacillus thuringiensis; Genoma; Mangue; Nucleotide sequences; Genome; Mangrove soils. |
Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/997160 |
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SÁBER, M. L.; ANDREOTE, F. D.; KAVAMURA, V. N.; FRIGHETTO, R. T. S.; TAKETANI, R. G.; MELO, I. S. de. |
Abstract: The impact of ultraviolet-B (UV-B) radiation on the culturable and unculturable bacterial communities were studied in field experiments using soybean plants grown under increased UV-B (UV-B+), negative control (UV-B-) and solar UV-B. Sampling of leaves exposed to UV-B was performed in different developmental stages of the plant. The data obtained demonstrate that UV-B radiation did not alter the culturable and unculturable bacterial community. In contrast, culture independent analysis revealed major differences between cultivars for the stage of development (cultivation time) of the plants. Regarding the bacterial communities of the phyllosphere, it is possible to observe a similar behavior of the analyzed groups (Bacteria, ?-Proteobacteria, and... |
Tipo: Separatas |
Palavras-chave: Ultraviolet-B; Pigmented bacteria; Survival; Radiação ultravioleta.; Bactéria; Soja.; Soybeans; Ultraviolet radiation; Phyllosphere. |
Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1008000 |
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SOARES JUNIOR, F. L.; DIAS, A. C. F.; FASANELLA, C. C.; TAKETANI, R. G.; LIMA, A. O. S.; MELO, I. S. de; ANDREOTE, F. D.. |
The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from amangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria,enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplementedwith 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33... |
Tipo: Separatas |
Palavras-chave: Cellulose; Endo-1; 4-B-D-glucanase Exo-1; 4-B-D-glucanase. salinity.. |
Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/981473 |
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DIAS, A. C. F.; TAKETANI, R. G.; ANDREOTE, F. D.; LUVIZOTTO, D. M.; SILVA, J. L. da; NASCIMENTO, R. dos S.; MELO, I. S. de. |
ABSTRACT: Mangrove forests encompass a group of trees species that inhabit the intertidal zones, where soil is characterized by the high salinity and low availability of oxygen. The phyllosphere of these trees represent the habitat provided on the aboveground parts of plants, supporting in a global scale, a large and complex microbial community. The structure of phyllosphere communities reflects immigration, survival and growth of microbial colonizers, which is influenced by numerous environmental factors in addition to leaf physical and chemical properties. Here, a combination of culture-base methods with PCR-DGGE was applied to test whether local or plant specific factors shape the bacterial community of the phyllosphere from three plant species... |
Tipo: Separatas |
Palavras-chave: Culture-independent profiling; Plant genotype; Surface leaves; Mangue; Bactéria; Planta; Phyllosphere; Mangrove forests. |
Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/942708 |
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DIAS, A. C. F.; COSTA, F. E. C.; ANDREOTE, F. D.; LACAVA, P. T.; TEIXEIRA, M. A.; ASSUMPÇÃO, L. C.; AZEVEDO, J. L.; MELO, I. S. de. |
Twenty endophytic bacteria were isolated from the meristematic tissues of three varieties of strawberry cultivated in vitro, and further identified, by FAME profile, into the genera Bacillus and Sphingopyxis. The strains were also characterized according to indole acetic acid production, phosphate solubilization and potential for plant growth promotion. Results showed that 15 strains produced high levels of IAA and all 20 showed potential for solubilizing inorganic phosphate. Plant growth promotion evaluated under greenhouse conditions revealed the ability of the strains to enhance the root number, length and dry weight and also the leaf number, petiole length and dry weight of the aerial portion. Seven Bacillus spp. strains promoted root development and... |
Tipo: Artigo de periódico |
Palavras-chave: Bactéria.. |
Ano: 2009 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/576702 |
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Registros recuperados: 35 | |
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