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In vitro detection of pathogenic Listeria monocytogenes from food sources by conventional, molecular and cell culture method BJM
Khan,J.A.; Rathore,R.S.; Khan,S.; Ahmad,I..
Among current in vitro methods for identification of pathogenic Listeria monocytogenes (L. monocytogenes) rely on growth in culture media, followed by isolation, and biochemical and serological identification. Now PCR (Polymerase Chain Reaction) has been used for the rapid, sensitive and specific detection of pathogenic L. monocytogenes. The pathogenicity of the organism is highly correlated with haemolytic factor known as listeriolysin O (LLO). A total of 400 samples from meat and 250 samples from raw milk and their products were collected from various local dairy farms, dairy units and butcheries in Bareilly, India. Pure isolates of L. monocytogenes obtained after enrichment in Buffered Listeria enrichment broth (BLEB) followed by plating onto Listeria...
Tipo: Info:eu-repo/semantics/article Palavras-chave: L. monocytogenes; Haemolysis; PCR; Vero cytotoxicity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300013
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