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Quantification of bovine cytokine gene expression using real-time RT-PCR methodology 74
Zaros,Lilian Giotto; Bricarello,Patrízia Ana; Amarante,Alessandro Francisco Talamini; Coutinho,Luiz Lehmann.
T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytokine gene expression; Real-time RT-PCR; Cattle; Gastrointestinal nematodes; Bovine cytokines.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012
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The occurrence of Crepidobothrium sp. (Cestoda, Proteocephalidae) in Bothrops moojeni (Hoge) (Serpentes, Viperidae) 104
Silva,Reinaldo José da; Karasawa,Andrea Satie Matsubara; Cherubini,André Luis; Barrella,Thomaz Henrique; Lopes,Carlos Alberto de Magalhães; Amarante,Alessandro Francisco Talamini.
The occurrence of Crepidobothrium sp.(Cestoda, Proteocephalidae) in the intestine of Bothrops moojeni (Hoge,1965)(Serpentes, Viperidae) is reported. The host snake was rescued from the fauna in Porto Primavera dam, Mato Grosso do Sul State, Brazil. The snake died in captivity on July 13,1999. At necropsy, 28 tapeworms were found in the snake intestine. The analysis of specimens morphology allowed the conclusion that they belong to the Crepidobothrium (Monticelli, 1900) genus. It was not possible to determine the Crepidobothrium species due to the lack of the gravid proglottids. This is the first report of B. moojeni as a host of cestodes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cestoda; Proteocephalidae; Crepidobothrium; Bothrops moojeni; Snake.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000200006
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