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| Okamoto,AS; Andreatti Filho,RL; Rocha,TS; Menconi,A; Marietto-Gonçalves,GA. |
| The expansion of global poultry production has increased the need to reduce or control the agents responsible for economic losses, including Salmonella spp. These bacteria are also of public health concern due to their potential to cause food poisoning, and, more recently, due to the antimicrobial resistance presented by these bacteria. Molecular biology is an important tool currently used in the diagnosis and research studies of main poultry diseases. The present studied analyzed 100 samples of Salmonella Enteritidis (SE) isolated from avian material aiming at detecting the class 1 integron gene, Integroninvolved in antimicrobial resistance, by means of polymerase chain reaction (PCR), and comparing it with plate inhibition test. Subsequently, SE samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Integron; Gene transfer; Resistance to antimicrobials; Salmonella Enteritidis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300009 |
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| Noujaim,JC; Andreatti Filho,RL; Lima,ET de; Okamoto,AS. |
| The expression of immune response as a leukocytic infiltrate by CD4+ and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks fed Lactobacillus spp or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis (SE) was studied using immunohistochemistry. Three hundred and twenty day-of-hatch broiler chicks were divided into four groups of 80 birds each and orally received L. reuteri, L. salivarius, L. acidophilus, or CM. Each group was subdivided into four subgroups of 20 birds each, classified as follows: a subgroup did not receive any oral treatment (negative control), subgroup treated with L. spp or CM, subgroup treated with L. spp or CM and challenged with SE, and subgroup only... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; Immunohistochemistry; Lymphocytes CD4+ and CD8+; Probiotic; Salmonella. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300008 |
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| Marietto-Gonçalves,GA; SMR,Curotto; Baptista,AAS; Donato,TC; Takahira,RK; Sequeira,JL; Andreatti Filho,RL. |
| Due to the constant evolution of industrial poultry production and the global emergence of bacterial resistance to antibiotics there has been an increasing interest in alternatives for the treatment of poultry salmonellosis, such as phage therapy and probiotics. The present study evaluated the effects of the oral administration of the bacteriophage P22 and of a probiotic, consisting of four Lactobacillus species, on the level of circulating heterophils containing a superoxide anion of one-day-old broilers challenged with Salmonella Typhimurium for seven days. It was concluded that the treatment with a probiotic with lactobacilli of broilers experimentally infected with Salmonella spp eliminates this pathogen by increasing the circulating levels of reactive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonellosis; Immune response; Respiratory burst; Poultry. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300005 |
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| Silva,IGO; Vellano,IHB; Moraes,AC; Lee,IM; Alvarenga,B; Milbradt,EL; Hataka,A; Okamoto,AS; Andreatti Filho,RL. |
| ABSTRACT The present study evaluated a probiotic and a competitive exclusion product injected in ovo on day 18 of incubation together with Marek's disease vaccine in eggs of 56-week-old broiler breeders. Three experiments were carried out. The first trial evaluated the effect of treatments on hatchability, cecal colonization of Salmonella Heidelberg (SH), and intestinal mucosa immunity (immunoglobulin A levels in the intestinal fluid). The second trial evaluated the viability of the microorganisms in the products inoculated in a solution containing diluent and Marek's disease vaccine. The third trial evaluated the colonization of the cecal microbiota in non-challenged chickens during first four days of life by culturing cecal samples under aerobic and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chickens; Competitive exclusion; In-ovo inoculation; Probiotic; Salmonella Heidelberg. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100019 |
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| Okamoto,AS; Andreatti Filho,RL; Lima,ET; Pereira,REP; Menconi,A; Rocha,TS; Marietto-Gonçalves,GA. |
| This study aimed at the antibody production by intestinal mucosa of broilers chicks were orally inoculated with Lactobacillus spp. at one and/or 21 days of age, and subsequently challenged with Salmonella enterica, subspecies enterica, serotype Enteritidis (S. Enteritidis). A total number of 288 drug-free broiler chicks was divided into 6 groups (groups A, B, C, D, E, and F), according to age at Lactobacillus spp. inoculation and SE challenge. The intestinal mucosa immune response was determined as the production of immunoglobulin A against S. Enteritidis, and evaluated by the Enzyme-Linked Immunosorbent Assay (ELISA) technique in intestinal washing fluid. Groups treated with Lactobacillus spp. presented higher IgA production only when the chicks were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicks; IgA; Lactobacillus; Salmonella. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000400009 |
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| Katayama,ERi; Donato,TC; Silva,A de P; Mazola,RP; Garcia,EA; Okamoto,AS; Andreatti Filho,RL. |
| Several studies point out that eggs are the main cause of human salmonellosis, particularly when poorly processed or eaten raw. In addition of causing public health problems, the presence of Salmonella hinders international food trade, and therefore, it is a health barrier. There are several predisposing factors for the contamination of internal egg content by Salmonella spp, including eggshell quality, which is related to dietary calcium (Ca) and phosphorus (P) levels. In this study, eggs from Japanese quails fed diets containing two different Ca (2.0 or 3.5%) or P (0.25 or 0.45%) levels were evaluated. Three production phases were used in the experiment: starter, intermediate, and final. The presence of Salmonella Enteriditis in the eggshell and internal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calcium; Quail production; Coturnix japonica; Phosphorus; Salmonellosis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000100005 |
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| Barros,MR; Andreatti Filho,RL; Lima,ET; Sampaio,HM; Crocci,AJ. |
| Avaliou-se a contaminação da casca e do conteúdo interno de ovos inoculados com Salmonella enterica sorovar enteritidis fagotipo 4, lavados com água de torneira (AT) ou solução de amônia quaternária (AQ) e armazenados a 8ºC e 25ºC. Duzentos e cinqüenta e dois ovos foram divididos em três grupos. Os tratamentos de cada grupo consistiram de imersão em AT, AQ a 25ºC e a 43ºC. Após a secagem natural, todos os grupos foram contaminados com solução de S. enteritidis. Seguindo-se a contaminação, cada grupo tratado foi estocado a 8ºC ou 25ºC, e a presença de S. enteritidis na casca e no conteúdo interno foi avaliada após zero, 24, 96 e 168 horas. A sanitização com AQ mostrou-se eficiente na redução de S. enteritidis nas cascas dos ovos. O armazenamento dos ovos a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovo; Salmonella enteritidis; Desinfecção; Temperatura de armazenamento. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2001000300003 |
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| Milbradt,EL; Okamoto,AS; Padovani,CR; Fascina,VB; Silva,TM; Altarúgio,R; Hataka,A; Schmidt,EMS; Andreatti Filho,RL. |
| ABSTRACT It is estimated that Salmonella causes one million illnesses in the United States annually, with 19,000 hospitalizations and 380 deaths. There are various serotypes of this bacterium, and the serotypes Typhimurium and Enteritidis are commonly isolated from outbreaks and, in most cases, present resistance to the antibiotics utilized in clinical medicine. One of the current theories to explain the emergence of bacterial resistance is the continuous use of antibiotics as growth promoters in animal feeds. Despite the lack of evidences that antibiotic growth promoters cause bacterial resistance, the poultry industry has explored strategies to reduce or to eliminate the use of antibiotic growth promoters in the production chain, including the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Commercial turkey; Competitive exclusion; Organic acid; Salmonella Enteritidis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400551 |
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