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Registros recuperados: 15
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An attempt to channel the transformation of vanillic acid into vanillin by controlling methoxyhydroquinone formation in Pycnoporus cinnabarinus with cellobiose Inra
Lesage-Meessen, L.; Haon, M.; Delattre, M.; Thibault, J.F.; Colonna Ceccaldi, B.; Asther, M..
The effects of adding cellobiose on the transformation of vanillic acid to vanillin by two strains of Pycnoporus cinnabarinus MUCL39532 and MUCL38467 were studied. When maltose was used as the carbon source in the culture medium, very high levels of methoxyhydroquinone were formed from vanillic acid. When cellobiose was used as the carbon source and/or added to the culture medium of P. cinnabarinus strains on day 3 just before vanillic acid was added, it channelled the vanillic acid metabolism via the reductive route leading to vanillin. Adding 3.5 g l−1 cellobiose to 3-day-old maltose cultures of P. cinnabarinus MUCL39532 and 2.5 g l−1 cellobiose to 3-day-old cellobiose cultures of P. cinnabarinus MUCL38467, yielded 510 mg l−1 and 560 mg l−1 vanillin...
Tipo: Journal Article Palavras-chave: PYCNOPORUS CINNABARINUS; METABOLISME ENZYMATIQUE; VANILLINE; ACIDE VANILLIQUE; COMPOSE AMINE; CELLOBIOSE.
Ano: 1997 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9700027779065989&uri=/notices/prodinra1/2010/09/
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Chromogenic substrates for feruloyl esterases Inra
Marmuse, L.; Asther, M.; Navarro, D.; Lesage-Meessen, L.; Asther, M.; Fort, S.; Driguez, H..
Chromogenic mono- and diferuloyl-butanetriol analogs were prepared by chemical syntheses and their efficiency was evaluated as substrates for feruloyl esterases from Aspergillus niger
Tipo: Journal Article Palavras-chave: ACTIVITE ENZYMATIQUE FERULOYL ESTERASE; CHROMOGENIC SUBSTRATE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200895c1f35e&uri=/notices/prodinra1/2008/06/
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Cloning and characterization of a tyrosinase gene from the white-rot fungus Pycnoporus sanguineus, and overproduction of the recombinant protein in Aspergillus niger Inra
Halaouli, S.; Record, E.; Casalot, L.; Hamdi, M.; Sigoillot, J.C.; Lomascolo, A.; Asther, M..
A new tyrosinase-encoding gene (2,204 bp) andthe corresponding cDNA (1,857 nucleotides) from thewhite-rot fungus Pycnoporus sanguineus BRFM49 werecloned. This gene consisted of seven exons and six intronsand encoded a predicted protein of 68 kDa, exceeding themature tyrosinase by 23 kDa. P. sanguineus tyrosinasecDNA was over-expressed in Aspergillus niger, a particularlysuitable fungus for heterologous expression of proteinsof biotechnological interest, under the control ofthe glyceraldehyde-3-phosphate-dehydrogenase promoteras strong and constitutive promoter. The glucoamylasepreprosequence of A. niger was used to target the secretion.This construction enabled the production of recombinanttyrosinase in the extracellular medium of A. niger. Theidentity of...
Tipo: Journal Article Palavras-chave: FUNGAL TYROSINASE.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20078d7fe834&uri=/notices/prodinra1/2010/09/
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Construction of engineered bifunctional enzymes and their overproduction in Aspergillus niger for improved enzymatic tools to degrade agricultural by-products Inra
Levasseur, A.; Navarro, D.; Punt, P.J.; Belaïch, J.P.; Asther, M.; Record, E..
Two chimeric enzymes, FLX and FLXLC, were designed and successfully overproduced in Aspergillus niger. FLX construct is composed of the sequences encoding the feruloyl esterase A (FAEA) fused to the endoxylanase B (XYNB) of A. niger. A C-terminal carbohydrate-binding module (CBM family 1) was grafted to FLX, generating the second hybrid enzyme, FLXLC. Between each partner, a hyperglycosylated linker was included to stabilize the constructs. Hybrid proteins were purified to homogeneity, and molecular masses were estimated to be 72 and 97 kDa for FLX and FLXLC, respectively. Integrity of hybrid enzymes was checked by immunodetection that showed a single form by using antibodies raised against FAEA and polyhistidine tag. Physicochemical properties of each...
Tipo: Journal Article Palavras-chave: SON DE CEREALE; ACTIVITE ENZYMATIQUE ASPERGILLUS NIGER.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20086808f0a6&uri=/notices/prodinra1/2008/08/
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Enzymatic saccharification of wheat straw for bioethanol production by a combined cellulase xylanase and feruloyl esterase treatment Inra
Tabka, M.G.; Herpoël-Gimbert, I.; Monod, F.; Asther, M.; Sigoillot, J.C..
The focus of this study was to improve conditions of use of fungal lignocellulolytic enzymes for conversion of lignocellulosic biomass to fermentable sugars for the production of bioethanol.Wheat straw was pre-treated by acid treatment with diluted sulfuric acid followed by steam explosion. Several enzymatic treatments implementing hydrolases (cellulases and xylanases from Trichoderma reesei, recombinant feruloyl esterase (FAE) from Aspergillus niger and oxidoreductases (laccases from Pycnoporus cinnabarinus) were investigated to the saccharification of exploded wheat straw. A synergistic effect between cellulases, FAE and xylanase was proven under a critical enzymatic concentration (10 U/g of cellulases, 3 U/g of xylanase and 10 U/g of FAE). The yield of...
Tipo: Journal Article Palavras-chave: ENZYME LIGNOLITIQUE FUNGAL ENZYME; SACCHARIFICATION; WHEAT STRAW; BIOETHANOL.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008ea41c692&uri=/notices/prodinra1/2008/06/
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Evidence of a new biotransformation pathway of p-coumaric acid into p-hydroxybenzaldehyde in Pycnoporus cinnabarinus Inra
Estrada Alvarado, I.; Lomascolo, A.; Navarro, D.; Delattre, M.; Asther, M.; Lesage-Meessen, L..
Pycnoporus cinnabarinus MUCL39533 was shown to be able to convert p-coumaric acid into p-hydroxybenzaldehyde, a component of high organoleptic note present in natural vanilla aroma. Use of phospholipid-enriched medium led to high-density cultures of P. cinnabarinus, since dry mycelial biomass was increased three-fold as compared to glucose medium. In the presence of phospholipids, 155 mg l–1 p-hydroxybenzaldehyde was produced as the major compound on culture day 13 with a molar yield of 26%. The degradation pathways of p-coumaric acid were investigated. Based on the different metabolites identified, an oxidative side-chain degradation pathway of p-coumaric acid conversion to p-hydroxybenzoic acid was suggested. This acid was further reduced...
Tipo: Journal Article Palavras-chave: PYCNOPORUS CINNABARINUS; AROME NATUREL; BENZALDEHYDE; VANILLINE.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300030628096328&uri=/notices/prodinra1/2010/09/
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Exploration of members of Aspergillus sections nigri, flavi, and terrei for feruloyl esterase production Inra
BOUZID, O.; RECORD, E.; Asther, M.; HAON, M.; NAVARRO, D.; ASTHER, M.; LESAGE-MEESSEN, L..
La capacité des espèces du genre Aspergillus sections Nigri, Flavi et Terrei à produire des féruloyl estérases a été étudiée en fonction de leur spécificité vis-à-vis d'hydroxycinnamates de méthyle synthétiques. Les féruloyl estérases de type A (FAEA), induites lors de croissance sur des produits céréaliers, sont connues pour montrer une préférence pour les moitiés aromatiques des substrats comportant des groupements méthoxy, comme le sinapinate de méthyle, tandis que les féruloyl estérases de type B (FAEB), induites lors de croissance sur pulpes de betterave, montrent une préférence pour les moitiés aromatiques des substrats comportant plusieurs groupements hydroxyle, comme l'acide caféique. Toutes les souches d'Aspergillus section Nigri (i.e., A. niger...
Tipo: Journal Article Palavras-chave: PULPE DE BETTERAVE; ADN GENOMIQUE ASPERGILLUS NIGER; ASPERGILLUS FLAVUS; ASPERGILLUS; NIGRI; FLAVI; TERREI.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007675254d9&uri=/notices/prodinra1/2007/05/
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FOLy: An integrated database for the classification and functional annotation of fungal oxidoreductases potentially involved in the degradation of lignin and related aromatic compounds Inra
Levasseur, A.; Piumi, F.; Coutinho, P.M.; Rancurel, C.; Asther, M.; Delattre, M.; Henrissat, B.; Pontarotti, P.; Asther, M.; Record, E..
The breakdown of lignin by fungi is a key step during carbon recycling in terrestrial ecosystems. This process is of great interest for green and white biotechnological applications. Given the importance of these enzymatic processes, we have classified the enzymes potentially involved in lignin catabolism into sequence-based families and integrated them in a newly developed database, designated Fungal Oxidative Lignin enzymes (FOLy). Families were defined after sequence similarity searches starting from protein sequences and validated by the convergence of results with biochemical experiments reported in the literature. The resulting database was applied as a tool for the functional annotation of genomes from different fungi, namely (i) the Basidiomycota...
Tipo: Journal Article Palavras-chave: COMPOSE AROMATIQUE; BASIDIOMYCETES; BASE DE DONNEES; ASCOMYCETES MELANIN SYNTHESIS; FUNCTIONAL CLASSIFICATION; LIGNIN DEGRADATION; FUNGAL GENOME; FUNCTIONAL ANNOTATION; DATABASE; GREEN BIOTECHNOLOGY.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20087e29edda&uri=/notices/prodinra1/2008/06/
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Fungal pretreatment by Phanerochaete chrysosporium to reduce the inhibition of methanogenesis by dehydroabietic acid Inra
Hodgson, J.; Laugero, C.; Leduc, R.; Asther, M.; Guiot, S.R..
The white-rot basidiomycete Phanerochaete chrysosporium BKM-F-1767 was tested for its capacity to degrade dehydroabietic acid (DHA). In anaerobic treatment, this molecule is the most recalcitrant member of the resin acid group, which is known to cause operational problems to anaerobic reactors treating pulp and paper industry wastewaters. In this study the effect of DHA on different parameters, such as growth, ligninolytic enzyme activity, extracellular protein production as well as both glycerol and ammonium consumption by the fungus, was determined. Although the above parameters were affected by the addition of DHA, the results show that the fungus could still produce significant titres of ligninolytic enzymes. The fungus removed 47% of the DHA initially...
Tipo: Journal Article Palavras-chave: PHANEROCHAETE CHRYSOSPORIUM; BASIDIOMYCETE; ACIDE DESHYDROABIETIQUE; BACTERIE ANAEROBIQUE; METHANOGENESE.
Ano: 1998 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0000025352083784&uri=/notices/prodinra1/2010/09/
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Hot extraction and characterization of a ligninlike fraction involved in larvicidal effects of decomposed leaf litter against mosquito Inra
Tilquin, M.; Meyran, J.C.; Asther, M.; Marigo, G..
Hot water-extraction was performed on decomposed leaf litter in order to solubilize the toxic fraction involved in the dietary interaction against mosquito larvae in subalpine breeding sites. The toxic fraction was partially extracted by water with an optimum temperature of 60°C and recovered in an insoluble form. Phytochemical characterization was achieved through differential enzymatic hydrolyses, using the laccase mediator delignifying system, and aluminum chloride chelation monitored by standard bioassays; comparative spectrophotometric analyses in ultraviolet light after solubilization in acetyl bromide; and comparative reversed-phase high-performance liquid chromatography of the phenolic aldehydes after alkaline nitrobenzene oxidation. The results...
Tipo: Journal Article Palavras-chave: AEDES AEGYPTI; DIPTERA; INSECTA; REGIME ALIMENTAIRE; INTERACTION PLANTE INSECTE; DECOMPOSITION VEGETALE; LITIERE VEGETALE; LARVE; LUTTE ANTIINSECTE; TEMPERATURE DE L'EAU; HPLC LEAF LITTER; LARVICIDAL ACTIVITY; LIGNINLIKE COMPOUNDS; HOT-WATER EXTRACTION; AEDES AEGYPTI.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300030697097629&uri=/notices/prodinra1/2010/11/
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Improvement of ferulic acid bioconversion into vanillin by use of high-density cultures of Pycnoporus cinnabarinus Inra
Oddou, J.; Stentelaire, C.; Lesage-Meesen, L.; Asther, M.; Colonna-Ceccaldi, B..
High-density cultures of Pycnoporus cinnabarinus were tested with a view to optimisation of ferulic acid bioconversion into vanillin. The dry weight was increased fourfold by using glucose, fructose or a mixture of glucose and phospholipids as carbon source instead of maltose, the carbon source previously used. 5 mmol l−1 vanillin, i.e. 760 mg l−1, was produced over 15 days with glucose-phospholipid medium. In contrast, formation of vanillin was lower using glucose or fructose compared to the maltose control. A bioreactor (2 l) with a glucose-phospholipid medium gave a molar yield of vanillin of 61% (4 mmol l−1). An alternative strategy was to grow the fungus on a glucose or fructose medium for 3 days, then switch to maltose during the bioconversion phase:...
Tipo: Journal Article Palavras-chave: BIOTRANSFORMATION; PYCNOPORUS CINNABARINUS; ACIDE FERULIQUE; VANILLINE.
Ano: 1999 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0000025350083782&uri=/notices/prodinra1/2010/09/
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Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application Inra
Record, E.; Asther, M.; Sigoillot, J.C.; Pagès, S.; Punt, P.J.; Delattre, M.; Haon, M.; Van den Hondel, C.A.M.J.J.; Sigoillot, J.C.; Lesage-Meessen, L.; Asther, M..
A well-known industrial fungus for enzyme production, Aspergillus niger, was selected to produce the feruloyl esterase FAEA by homologous overexpression for pulp bleaching application. The gpd gene promoter was used to drive FAEA expression. Changing the nature and concentration of the carbon source nature (maltose to glucose; from 2.5 to 60 g l(-1)), improved FAEA activity 24.5-fold and a yield of 1 g l(-1) of the corresponding protein in the culture medium was achieved. The secreted FAEA was purified 3.5-fold to homogeneity in a two-step purification procedure with a recovery of 69%. The overproduced protein was characterised and presented properties in good agreement with those of native FAEA. The recombinant FAEA was tested for wheat straw pulp...
Tipo: Journal Article Palavras-chave: BLANCHIMENT FERULOYL ESTERASE; WHEAT-STRAW PULP; ENZYMATIC TREATMENT; LACCASE; XYLANASE.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20071bf46680&uri=/notices/prodinra1/2010/09/
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Secretome analysis of Phanerochaete chrysosporium strains CIRM-BRFM41 grown on softwood Inra
Ravalason, H.; Jan, G.; Mollé, D.; Pasco, M.; Coutinho, P.M.; Lapierre, C.; Pollet, B.; Bertaud, F.; Petit-Conil, M.; Grisel, S.; Sigoillot, J.C.; Asther, M.; Gimbert, I..
Abstract Proteomic analysis was performed to determine and differentiate the composition of the secretomes of Phanerochaete chrysosporium CIRM-BRFM41, a peroxidasehypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions.Extracellular proteins from both cultures were analyzed by bidimensional gel electrophoresis and matrix-assisted laserdesorption/ionization time-of-flight tandem mass spectrometry.A total of 37 spots were identified. The secretome in liquid synthetic medium comprised mainly peroxidases,while several wood-degrading enzymes and enzymes involved in fungal metabolism were detected in biopulping cultures on softwood. This prompted an analysis of the impact of secretome modulation in the presence...
Tipo: Journal Article Palavras-chave:  SECRETOME SOFTWOOD CHEMICAL PULPING; Phanerochaete chrysosporium.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20092bd81e9f&uri=/notices/prodinra1/2010/09/
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Tracking the connection between evolutionary and functional shifts using the fungal lipase/feruloyl esterase A family Inra
LEVASSEUR, A.; Gouret, P.; LESAGE-MEESSEN, L.; Asther, M.; ASTHER, M.; RECORD, E.; Pontarotti, P..
BackgroundThere have been many claims of adaptive molecular evolution, but what role does positive selection play in functional divergence? The aim of this study was to test the relationship between evolutionary and functional shifts with special emphasis on the role of the environment. For this purpose, we studied the fungal lipase/feruloyl esterase A family, whose functional diversification makes it a very promising candidate.ResultsThe results suggested functional shift following a duplication event where neofunctionalisation of feruloyl esterase A had occurred with conservation of the ancestral lipase function. Evolutionary shift was detected using the branch-site model for testing positive selection on individual codons along specific lineages....
Tipo: Journal Article Palavras-chave: ADAPTATION MOLECULAIRE; ASPERGILLUS.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007ed47815c&uri=/notices/prodinra1/2009/10/
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Transgenic rice as a novel production system for Melanocarpus and Pycnoporus laccases Inra
de Wilde, C.; Uzan, E.; Zhou, Z.; Kruus, K.; Andberg, M.; Buchert, J.; Record, E.; Asther, M.; Lomascolo, A..
Laccases have numerous biotechnological applications, among them food processing. The widespread use of laccases has increased the demand for an inexpensive and safe source of recombinant enzyme. We explored the use of a rice-based system for the production of two fungal laccases derived from the ascomycete Melanocarpus albomyces and the basidiomycete Pycnoporus cinnabarinus. High-expression levels of active recombinant laccases were achieved by targeting expression to the endosperm of rice seeds. The laccase cDNAs were fused to a plant-derived signal sequence for targeting to the secretory pathway, and placed under the control of a constitutive seed-specific promoter fused to an intron for enhanced expression. This construct enabled the recovery of on...
Tipo: Journal Article Palavras-chave: PLANTE TRANSGENIQUE; BIOTECHNOLOGIE INDUSTRIELLE; PROPRIETE BIOCHIMIQUE; ASCOMYCETE; BASIDIOMYCETE LACCASE; RECOMBINANT FUNGAL ENZYME; RICE; HETEROLOGOUS PROTEIN; TRANSGENIC PLANT; BIOCHEMICAL PROPERTIES.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008c973f6c6&uri=/notices/prodinra1/2008/10/
Registros recuperados: 15
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