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| AGOSTINETTO, D.; BENEMANN, D. P.; CECHIN, J.; NOHATTO, M. A.; LANGARO, A. C.; PIASECKI, C.; VARGAS, L.. |
| Herbicides are stressors that can have negative effects on plants. In Oryza sativa (L.), differential gene expression may be evalu-ated through real-time reverse transcription quantitative poly-merase chain reaction (RT-qPCR). The aim of this study was to evaluate the stability of candidate reference genes and to quan-tify the relative expression of oxidative stress genes at different times (12, 24, 48, and 96 hours after treatment [HAT]) with penoxsulam, cyhalofop-butyl, and bentazon herbicides. Norm-Finder, BestKeeper, and GeNorm software and the compara-tive ∆Ct method were used to assess expression stability and to determine the RT-qPCR threshold values of the candidate reference genes. The UBQ5 gene was the most stable among the reference... |
| Tipo: Artigo de periódico |
Palavras-chave: Oryza Sativa; Rice; Gene expression; Oxidative stress; Herbicides. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120636 |
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| CECHIN, J.; PIASECKI, C.; BENEMANN, D. P.; KREMER, F. S.; GALLI, V.; MAIA, L. C.; AGOSTINETTO, D.; VARGAS, L.. |
| Italian ryegrass (Lolium multiflorum; LOLMU) is one of the most troublesome weeds in temperate regions in the world. This weed species interfere with wheat, corn, rye, and oat, causing significant crop yield losses. This species has evolved glyphosate resistance, making it difcult to control. The mechanisms of glyphosate resistance are still unknown, and an understanding thereof will favor the development of new strategies of management. The present study is the first transcriptome study in LOLMU using glyphosate-resistant and -sensitive biotypes, aiming to identify and to provide a list of the candidate target genes related to glyphosate resistance mechanism. The transcriptome was assembled de novo, producing 87,433 contigs with an N50 of 740 bp and an... |
| Tipo: Artigo de periódico |
Palavras-chave: Italian ryegrass; Next-generation sequencing; RNA-Seq; Diferential gene expression; Resistance mechanism. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129871 |
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| PIASECKI, C.; YANG, Y.; BENEMANN, D. P.; KREMER, F. S.; GALLI, V.; MILLWOOD, R. J.; CECHIN, J.; AGOSTINETTO, D.; MAIA, L. C.; VARGAS, L.; STEWART JR, C. N.. |
| Tipo: Artigo de periódico |
Palavras-chave: Buva; Erva Daninha; Herbicida; Conyza bonariensis; Glyphosate; Herbicide resistance; Weeds; Gene expression. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113359 |
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