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Anticandidal effect of endophytic bacteria isolated from Equisetum arvense L. against Candida albicans and Candida glabrata BABT
Das,Gitishree; Patra,Jayanta Kumar; Choi,Jaehyuk; Baek,Kwang-Hyun.
ABSTRACT Equisetum arvense, a fern species possesses a number of pharmaceutical prospective. In the present study, a total of 103 endophytic bacteria isolated from E. arvense and were evaluated for their anticandidal property against five Candida species, two C. albicans, C. glabrata, C. saitoana and C. geochares. Out of them fifty one were identified as per the morphological and molecular characterisation using 16S rRNA gene sequencing and among them, ten promising endophytic bacteria were mentioned in the present study. Among ten endophytic bacteria, Psychrobacillus insolitus and Curtobacterium oceanosedimentum exerted highest anticandidal effect against C. albicans KACC 30062 and C. glabrata KBNO6P00368, with diameter of inhibition zones of 21.30±0.41...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticandidal effect; Endophytic bacteria; Equisetum arvense; Candida albicans; Candida glabrata.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100455
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Production of gaba (γ - aminobutyric acid) by microorganisms: a review BJM
Dhakal,Radhika; Bajpai,Vivek K.; Baek,Kwang-Hyun.
GABA (γ-aminobutyric acid) is a four carbon non-protein amino acid that is widely distributed in plants, animals and microorganisms. As a metabolic product of plants and microorganisms produced by the decarboxylation of glutamic acid, GABA functions as an inhibitory neurotransmitter in the brain that directly affects the personality and the stress management. A wide range of traditional foods produced by microbial fermentation contain GABA, in which GABA is safe and eco-friendly, and also has the possibility of providing new health-benefited products enriched with GABA. Synthesis of GABA is catalyzed by glutamate decarboxylase, therefore, the optimal fermentation condition is mainly based on the biochemical properties of the enzyme. Major GABA producing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GABA (γ-aminobutyric acid); Microorganisms; Optimal conditions; Lactic acid bacteria; GABA-enriched food.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400001
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Quantitative real-time PCR method to detect changes in specific transcript and total RNA amounts Electron. J. Biotechnol.
Baek,Kwang-Hyun; Skinner,Daniel Z.
Quantitative real-time PCR (qRT-PCR), used in conjunction with reverse transcriptase, has been applied to the determination of the number of copies of a transcript per unit mass of RNA, but did not indicate any change in the amount of total RNA per mass of tissue. In the present work, we described a simple method to use qRT-PCR to estimate the change in the amount of total RNA per unit mass of wheat (Triticum aestivum L.) tissue in response to cold temperature. Three qRT-PCR templates, i.e. control, cold-exposed, and one of RNA extracted from a sample consisting of equal masses of control and cold-exposed tissue, were analyzed. The number of copies of target transcript per unit mass of RNA was estimated from the three samples using standard qRT-PCR...
Tipo: Journal article
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000100008
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