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Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities 5
Balti, Rafik; Le Balc'H, Romain; Brodu, Nicolas; Gilbert, Marthe; Le Gouic, Benjamin; Le Gall, Sophie; Sinquin, Corinne; Masse, Anthony.
Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 µm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L.h-1. m-2 were obtained during the concentration at 4 bar for respectively cross-flow velocities inside the membrane lumen equal to 2.5, 3.3 and 4.2 m.s-1; 49.7 L.h-1. m-2 for the diafiltration at 3.3 m.s-1. Permeate fluxes were correctly predicted from polysaccharide concentrations (10 % deviation). Volume reduction factors higher than 7.8 were reached. Rejection rates of polysaccharides and proteins varied according to the cross-flow velocities. Thus, the EPS recovery rate or...
Tipo: Text Palavras-chave: Membrane filtration; Exopolysaccharides; Microalga; Concentration; Diafiltration.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00445/55694/57360.pdf
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Pre-purification by membrane filtration of paralytic shellfish toxins from Alexandrium minutum dinoflagellate 5
Balti, Rafik; Brodu, Nicolas; Zhang, Jiaxuan; Amzil, Zouher; Drouin, Delphine; Sechet, Veronique; Masse, Anthony.
The Paralytic shellfish neurotoxins (PST) are of increasing interest for biomedical applications. The chemical synthesis is often complex and expensive that’s why the purification by membrane filtration of PST from Alexandrium minutum dinoflagellate was investigated. Disrupted micro-alga cells by ultrasonic treatment were diafiltred to let pass toxins through an ultrafiltration membrane. Then, the mean permeate was concentrated and diafiltrated by nanofiltration. Mean permeate fluxes equal to 187, 135 and 135 L.h–1.m-2 were obtained during the first diafiltration, the concentration step and the final diafiltration respectively. Up to 57 % (mol/mol) and 78 % (mol/mol) of organic matters and salts were removed respectively. Divalent ions were sparsely...
Tipo: Text Palavras-chave: Paralytic shellfish poisoning; Nanofiltration; Membrane; Alexandrium minutum; Purification.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00451/56286/57865.pdf
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