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Comparison of High Performance Liquid Chromatography with Fluorescence Detector and with Tandem Mass Spectrometry methods for detection and quantification of Ochratoxin A in green and roasted coffee beans BABT
Bandeira,Raquel Duarte da Costa Cunha; Uekane,Thais Matsue; Cunha,Carolina Passos da; Rodrigues,Janaina Marques; la Cruz,Marcus Henrique Campino de; Godoy,Ronoel Luiz de Oliveira; Fioravante,Andreia de Lima.
Two analytical methods for the determination and confirmation of ochratoxin A (OTA) in green and roasted coffee samples were compared. Sample extraction and clean-up were based on liquid-liquid phase extraction and immunoaffinity column. The detection of OTA was carried out with the high performance liquid chromatography (HPLC) combined either with fluorescence detection (FLD), or positive electrospray ionization (ESI+) coupled with tandem mass spectrometry (MS-MS). The results obtained with the LC-ESI-MS/MS were specific and more sensitive, with the advantages in terms of unambiguous analyte identification, when compared with the HPLC-FLD.
Tipo: Info:eu-repo/semantics/article Palavras-chave: HPLC-FLD; LC-MS/MS; Green Coffee and Roasted Coffee.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600004
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Development and validation of a method for detection and quantification of ochratoxin A in green coffee using liquid chromatography coupled to mass spectrometry Ciênc. Tecnol. Aliment.
Bandeira,Raquel Duarte da Costa Cunha; Uekane,Thaís Matsue; Cunha,Carolina Passos da; Cunha,Valnei Smarçaro da; Rodrigues,Janaína Marques; Godoy,Ronoel Luiz de Oliveira; la Cruz,Marcus Henrique Campino de.
A method using Liquid Chromatography Tanden Mass Spectrometry (LC-MS/MS) with matrix-matched calibration curve was developed and validated for determining ochratoxin A (OTA) in green coffee. Linearity was found between 3.0 and 23.0 ng.g-1. Mean recoveries ranged between 90.45% and 108.81%; the relative standard deviation under repeatability and intermediate precision conditions ranged from 5.39% to 9.94% and from 2.20% to 14.34%, respectively. The limits of detection and quantification were 1.2 ng.g-1 and 3.0 ng.g-¹, respectively. The method developed was suitable and contributed to the field of mycotoxin analysis, and it will be used for future production of the Certified Reference Material (CRM) for OTA in coffee.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee; Mycotoxin; Validation; HPLC; MS/MS.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400021
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