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| Vargas,A.M.; Barros,R.P.A.; Zampieri,R.A.; Okamoto,M.M.; de Carvalho Papa,P.; Machado,U.F.. |
| The GLUT4 transporter plays a key role in insulin-induced glucose uptake, which is impaired in insulin resistance. The objective of the present study was to investigate the tissue content and the subcellular distribution of GLUT4 protein in 4- to 12-year-old control, obese and insulin-treated diabetic mongrel female dogs (4 animals per group). The parametrial white adipose tissue was sampled and processed to obtain both plasma membrane and microsome subcellular fractions for GLUT4 analysis by Western blotting. There was no significant difference in glycemia and insulinemia between control and obese animals. Diabetic dogs showed hyperglycemia (369.9 ± 89.9 mg/dl). Compared to control, the plasma membrane GLUT4, reported per g tissue, was reduced by 55% (P... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Diabetes; Obesity; Insulin resistance; GLUT4; Adipose tissue; Plasma membrane. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700020 |
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