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Registros recuperados: 14
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Comparative study of shell shape and muscle scar pigmentation in the closely related cupped oysters Crassostrea angulata, C. gigas and their reciprocal hybrids ArchiMer
Batista, Frederico; Ben-hamadou, Radhouan; Fonseca, Vera; Taris, Nicolas; Ruano, Francisco; Reis-henriques, Maria; Boudry, Pierre.
The taxonomic status of the cupped oysters Crassostrea angulata and C. gigas has received considerable attention in the last decades. Based on larval shell morphology, experimental hybridization, allozymes and nuclear DNA studies several authors have considered these two taxa as being synonymous. However, mitochondrial data showed clear genetic differences between the two taxa. In addition, microsatellite-based studies and cytogenetic studies have also provided evidence that supports their differentiation. Considerable differences have also been observed at the phenotypic level in terms of growth rate and ecophysiological parameters. In the present study, C. angulata from Sado estuary (Portugal) and C. gigas from Seudre estuary (France) were collected and...
Tipo: Text Palavras-chave: Crassostrea gigas; Crassostrea angulata; Hybridization; Shell pigmentation; Shell morphology.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3904.pdf
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Conséquences génétiques de la production de larves d'huîtres en écloserie : étude des processus de dérive et de sélection ArchiMer
Taris, Nicolas; Sauvage, Christopher; Batista, Frederico; Baron, Sophie; Ernande, Bruno; Haffray, Pierrick; Boudry, Pierre.
Previous studies have shown heritable variation in larval developmental traits in the Pacific oyster Crassostrea gigas. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors, specific to hatcheries, were examined: the effect of discarding the smallest larvae (i.e. culling) and the effect of temperature (20°C versus 26°C). A mixed-family approach was used in order to infer the genetic composition of larval populations and family assignment, limiting possible environmental bias and allowing the study of a relatively large number of families using a limited number of larval tanks. Our results show that three multiplexed highly polymorphic microsatellite markers are a powerful tool for family assignment and,...
Tipo: Text Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-1505.pdf
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Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review ArchiMer
Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan.
Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1...
Tipo: Text Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf
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Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae ArchiMer
Batista, Frederico; Taris, Nicolas; Boudry, Pierre; Renault, Tristan.
A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
Tipo: Text Palavras-chave: DNA extraction; Detection; Larvae; Oyster; OsHV 1; Herpesvirus.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2916.pdf
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Effects of genetic drift and selection at larval stage resulting from hatchery practices in the Pacific oyster (Crassostrea gigas) ArchiMer
Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Batista, Frederico; Boudry, Pierre.
Hatchery techniques are now well handled in an increasing number of aquaculture species. However, unintentional effects of domestication often remain poorly documented. In this context, we studied how current hatchery practices may genetically influence larval traits for which significant genetic variation had previously been reported in the Pacific oyster. Two main factors were studied: culling (i.e. discarding the smallest larvae) and rearing temperature (26°C versus 20°C). In parallel, we estimated genetic diversity of broodstock populations sampled in French hatcheries. Finally, we compared larval development and settlement success in the progeny of one of these hatchery broodstock, which had been selected for seven generations, with those of wild...
Tipo: Text Palavras-chave: Genetic drift; Selection; Genetic; Crassostrea gigas; Oysters; Hatchery techniques.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3362.pdf
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Evidence for the presence of the Portuguese oyster, Crassostrea angulata, in northern China ArchiMer
Lapegue, Sylvie; Batista, Frederico; Heurtebise, Serge; Yu, Ziniu; Boudry, Pierre.
The Pacific oyster, Crassostrea gigas (Thunberg), and the Portuguese oyster, C. angulata (Lamarck), are two closely related taxa. Although these two taxa were both introduced from Asia into Europe. one (C. gigas) was voluntarily introduced in the early 1970s, whereas the other (C. angulata) was presumed to be present in Europe for at least four centuries, but nearly disappeared because of disease. Few C. angulata populations remained in southern Portugal, Spain and Morocco and their putative origin was traced in Taiwan. The present paper reports evidence for its presence in Northern China. We reanalyzed recently published mitochondrial cytochrome oxidase C subunit I (COI) sequence data from presumed Dalianwan oysters (C. talienwhanensis) and compared them...
Tipo: Text Palavras-chave: Phylogeography; Cytochrome oxidase C subunit 1 gene; Crassostrea talienwhanensis; Gigas; Crosstrea; Crassostrea angulata; Cupped oysters.
Ano: 2004 URL: https://archimer.ifremer.fr/doc/2004/publication-3173.pdf
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Hybridization and morphological differences between the two closely related oyster taxa Crassostrea angulata and C. gigas ArchiMer
Batista, Frederico; Fonseca, Vera; Ben-hamadou, Radhouan; Taris, Nicolas; Henriques, Maria; Boudry, Pierre.
Unlike in some crops, no large heterotic effects have been observed in most farmed animals. Interestingly, significant hybrid vigour for some traits has been reported in the Pacific oyster, Crassostrea gigas. Crossbreeding between closely related taxa can be seen as an efficient way to exploit the dominance component of the genetic variance of a trait. Factorial crosses between the Portuguese oyster C. angulata (from Sado estuary, Portugal) and C. gigas (from Seudre estuary, France) were done to evaluate the aquaculture potential of hybrids. Juveniles of the different progenies were reared in Ria Formosa (Portugal) under usual farming conditions. The genetic confirmation of all progenies was done using mitochondrial and nuclear markers in order to detect...
Tipo: Text Palavras-chave: Hybridization; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3429.pdf
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Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles following its introduction for aquaculture production ArchiMer
Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andrew; King, J. W.; Turner, J. R.; Lapegue, Sylvie.
The Pacific oyster, Crassostrea gigas, native to northeast Asia, has been translocated widely for the purpose of aquaculture and is among the most important cultured shellfish. C. gigas has a complex history of introductions in Europe. In France, massive introduction was performed in the early 1970’s, allowing massive spatfall in favourable areas to become the basis of the local oyster industry. In the U.K, hatchery propagation was used to supply local production. Some seed was also exported to Northern Europe were demographically independent populations have been reported in the last decades. In the present study 22 sites were sampled in the British Isles, Denmark, France and Spain in order to assess the genetic diversity and population differentiation...
Tipo: Text Palavras-chave: Invasion genetics; Pacific oyster; Crassostrea gigas; British Isles.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27814/26007.pdf
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Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers ArchiMer
Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andy; King, Jonathan W.; Turner, John R.; Lapegue, Sylvie.
The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and...
Tipo: Text Palavras-chave: Invasion genetics; Marine mollusc; Microsatellites; MtDNA; Pacific oyster; Crassostrea gigas.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00268/37973/36052.pdf
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Is the Portuguese oyster Crassostrea angulata in Southern Europe endangered by the expansion of the Pacific oyster C. gigas? ArchiMer
Batista, Frederico; Boudry, Pierre; Lapegue, Sylvie; Heurtebise, Serge; Monteiro, C. C..
The Portuguese oyster, Crassostrea angulata, was introduced from Portugal to the French Atlantic Coast in the 1860s. C. angulata quickly settled and expanded and leaded to the development of a new aquacultural industry in France. In the late 1960s, mortality associated with the detection of an iridovirus, led to the wipe out of C. angulata from French Atlantic waters and to the massive introduction of C. gigas to sustain production. In Southern Europe, similar symptoms were also observed in natural stocks of C. angulata from Sado River (Portugal) and from the area of Cadiz (Spain). Nowadays, only very few «pure» populations of C. angulata remain in southern Europe. These populations are potentially endangered by the current expansion of C. gigas...
Tipo: Text Palavras-chave: Selection; Hybridization; Settlement pattern; Population genetic; Genetic; Oysters; Crassostrea angulata; Crassostrea gigas.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3310.pdf
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Phylogeographic study of the dwarf oyster, Ostreola stentina, from Morocco, Portugal and Tunisia: evidence of a geographic disjunction with the closely related taxa, Ostrea aupouria and Ostreola equestris ArchiMer
Lapegue, Sylvie; Ben Salah, Ines; Batista, Frederico; Heurtebise, Serge; Neifar, L; Boudry, Pierre.
Despite the economic importance of oysters due to the high aquaculture production of several species, the current knowledge of oyster phylogeny and systematics is still fragmentary. In Europe, Ostrea edulis, the European flat oyster, and Ostreola stentina, the Provence oyster or dwarf oyster, are both present along the European and African, Atlantic and Mediterranean, coasts. In order to document the relationship not only between O. stentina and O. edulis, but also with the other Ostrea and Ostreola species, we performed a sequence analysis of the 16S mitochondrial fragment (16S rDNA: the large subunit rRNA-coding gene) and the COI fragment (COI: cytochrome oxidase subunit I). Oysters were sampled from populations in Portugal (two populations), Tunisia...
Tipo: Text Palavras-chave: Tunisia; Portugal; Marocco; Ostreola stentina; Ostreola equestris; Ostrea aupouria; Phylogeographic study.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1912.pdf
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Response to unintentional selection for faster development associated with inbreeding depression in Crassostrea gigas larvae, resulting from a broodstock selective breeding plan ArchiMer
Taris, Nicolas; Batista, Frederico; Marissal, Eric; Boudry, Pierre.
Direct and indirect consequences of selective breeding in marine bivalves still remain largely unexplored. For species with two-phase life cycle, like the Pacific oyster (Crassostrea gigas), most studies have focused on juvenile and adult stages, but relatively few have focused on juvenile and adult stages, but relatively few have considered the larval stage, especially in a domestication context. We assessed the impact of hatchery practices on larval traits, notably on larval growth (due to the culling of slow growing larvae), by the study of larval progenies. Larvae originating from crosses using parental oysters both from natural beds and from hatchery broodstock which had been selected for adult growth and shell shape for seven generations. A set of...
Tipo: Text Palavras-chave: Inbreeding depresion; Larvae stage; Genetic variability; Hatchery; Domestication; Genetic; Crassostrea gigas; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3337.pdf
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Taxonomie de l'huître naine Ostreola stentina ArchiMer
Lapegue, Sylvie; Ben Salah, Inès; Batista, Frederico; Heurtebise, Serge; Neifar, Lassad; Boudry, Pierre.
Despite the economic importance of numerous oyster species throughout the world, our knowledge of their systematics and phylogeography remains fragmentary. In Europe, the indigenous species, Ostrea edulis, suffered overexploitation as well as two successive parasitosis over the last century. However, this species is still cultivated in Europe, and especially in Spain and France. Its distribution area stretches from the Atlantic coasts of Europe to the north of Africa as well as around the Mediterranean and Black Sea. Its Mediterranean and South Atlantic distribution area is shared with another indigenous species, the dwarf oyster, Ostreola stentina. Its very small adult size (a few centimetres) makes its culture pointless from a commercial point of view....
Tipo: Text Palavras-chave: Ostreola stentina; Oyster; Taxonomy; Ostreola stentina; Huître; Taxonomie.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3364.pdf
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The taxonomic status and origin of the Portuguese oyster Crassostea angulata (Lamark, 1819) ArchiMer
Batista, Frederico; Leitao, Alexandra; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre.
The taxonomic status of the Portuguese oyster Crassostrea angulata and the Pacific oyster Crassostrea gigas has often been a matter of controversy. Based on larval shell morphology, experimental hybridisation and electrophoretic studies of enzyme polymorphism several authors have considered these two species as being synonymous. During the recent years, several genetic studies based on mitochondrial DNA and microsatellites data have provided evidences that the two taxa are genetically distinct although closely related. Furthermore, karyotype analyses have also highlighted the close genetic similarity of these taxa in comparison with other cupped oyster species. However, a comparative analysis of restriction enzymes ideograms revealed differences between...
Tipo: Text Palavras-chave: Geographical origin; Phylogenetic; Genetic; Taxonomic status; Crassostrea angulata; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3433.pdf
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