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A new device for in situ video and fluorescence analysis of marine particles. Applications to phytoplankton studies. ArchiMer
Lunven, Michel; Lehaitre, Michel; Nascimben, Eric; Rouvillain, Julien; Berric, Roger; Le Gall, Erwan; Gentien, Patrick.
A new video-microscope has been developed for in situ investigation on marine particles. A field of view is produced by a laser beam at 473 nm. Individual cells or particles that entered this field of view appear as individual diffraction-limited spots of light, which are resolved from the dark background. A mobile high-pass optical filter (610 nm) can be controlled and displaced in front of the CCD camera, allowing simultaneously imaging and discrimination between fluorescing and non-fluorescing particles. We have the possibility to add a band pass filter for phycoerytrin detection. The system allows white light visualisation of particles ranging from 10 µm to several millimetres, depending on the zoom magnification. With the 610 nm high pass filter, the...
Tipo: Text Palavras-chave: Surveillance; Phytoplancton; Phycotoxine.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00144/25495/23649.pdf
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Nutrient and phytoplankton distribution in the Loire River plume (Bay of Biscay, France) resolved by a new Fine Scale Sampler ArchiMer
Lunven, Michel; Guillaud, Jean-francois; Youenou, Agnes; Crassous, Marie-pierre; Berric, Roger; Le Gall, Erwan; Kerouel, Roger; Labry, Claire; Aminot, Alain.
A new Fine Scale Sampler (FSS) was designed and used in the Loire River plume (Bay of Biscay, France) in order to study phytoplankton distribution in highly stratified water columns. The FSS consists of a linear array of 15 sampling bottles, set horizontally at 20 cm intervals. Data acquired from the CTD and fluorescence probe of the FSS enable it to be precisely positioned at the depth of the highest density gradient or at the chlorophyll a maximum. The FSS made it possible to investigate fine scale vertical distribution of phytoplankton and relationships with water density, nutrient concentrations or light availability, for spring and summer conditions. Under spring conditions, the entire upper layer exhibited a dominant diatom population (Chaetoceros...
Tipo: Text Palavras-chave: Plankton patchiness; Density discontinuity; Sampling; Thin layer; Nutrients; Phytoplankton.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-533.pdf
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Vertical distribution of phytoplankton communities and relationships with physico-chemical parameters resolved by a fine scale sampler ArchiMer
Lunven, Michel; Guillaud, Jean-francois; Youenou, Agnes; Crassous, Marie-pierre; Berric, Roger; Le Gall, Erwan; Kerouel, Roger; Labry, Claire; Aminot, Alain.
Many studies in coastal areas have shown the existence of thin chemical and biological layers. Their thickness and vertical extension are mainly controlled by hydrological and physical forcing. An accurate description of the phytoplankton communities growing within these layers is difficult to obtain without a well-adapted sampling system. A new Fine Scale Sampler (FSS) has been designed in Ifremer in order to study phytoplankton distribution and associated dissolved properties in high stratified water column. This FSS consists in a vertical array of 15 sampling bottles, horizontally laid with 20 cm intervals. Data acquired from a CTD and Fluorescence probe provide a precise FSS positioning at the depth of highest density gradient or at chlorophyll a...
Tipo: Text Palavras-chave: Surveillance; Littoral; Phytoplancton; Phycotoxine.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00144/25493/23647.pdf
Registros recuperados: 3
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