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Secretome analysis of Phanerochaete chrysosporium strains CIRM-BRFM41 grown on softwood Inra
Ravalason, H.; Jan, G.; Mollé, D.; Pasco, M.; Coutinho, P.M.; Lapierre, C.; Pollet, B.; Bertaud, F.; Petit-Conil, M.; Grisel, S.; Sigoillot, J.C.; Asther, M.; Gimbert, I..
Abstract Proteomic analysis was performed to determine and differentiate the composition of the secretomes of Phanerochaete chrysosporium CIRM-BRFM41, a peroxidasehypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions.Extracellular proteins from both cultures were analyzed by bidimensional gel electrophoresis and matrix-assisted laserdesorption/ionization time-of-flight tandem mass spectrometry.A total of 37 spots were identified. The secretome in liquid synthetic medium comprised mainly peroxidases,while several wood-degrading enzymes and enzymes involved in fungal metabolism were detected in biopulping cultures on softwood. This prompted an analysis of the impact of secretome modulation in the presence...
Tipo: Journal Article Palavras-chave:  SECRETOME SOFTWOOD CHEMICAL PULPING; Phanerochaete chrysosporium.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20092bd81e9f&uri=/notices/prodinra1/2010/09/
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