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Production, purification and characterization of a thermostable β-1,3-glucanase (laminarinase) produced by Moniliophthora perniciosa Anais da ABC (AABC)
Sena,Amanda R.; Júnior,Gildomar L.V.; Góes Neto,Aristóteles; Taranto,Alex G.; Pirovani,Carlos P.; Cascardo,Júlio C.M.; Zingali,Russolina B.; Bezerra,Marcos A.; Assis,Sandra A..
The enzyme glucanase from Moniliophthora perniciosa was produced in liquid medium and purified from the culture supernatant. A multivariate statistical approach (Response Surface Methodology - RSM) was employed to evaluate the effect of variables, including inducer (yeast extract) and fermentation time, on secreted glucanase activities M. perniciosa detected in the culture medium. The crude enzyme present in the supernatant was purified in two steps: precipitation with ammonium sulfate (70%) and gel filtration chromatography on Sephacryl S-200. The best inducer and fermentation time for glucanase activities were 5.9 g L-1 and 13 days, respectively. The results revealed three different isoforms (GLUI, GLUII and GLUIII) with purification factors of 4.33,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucanase; Moniliophthora perniciosa; Production; Kinetic characterization; Purification; Heat stability.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200019
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Use of Doehlert and constrained mixture designs in the development of a photo-oxidation procedure using UV radiation/H2O2 for decomposition of landfill leachate samples and determination of metals by flame atomic absorption spectrometry Anais da ABC (AABC)
Bezerra,Marcos A.; Souza,Antônio D.S.; Oliveira,Rafael V.; Oliveira,Djalma M.; Cardoso,Luiz A.M.; Sousa Filho,Hélio R..
This work proposes the use of photo-oxidation degradation with UV radiation/H2O2 as sample treatment for the determination of Fe, Zn, Mn, Ni and Co in municipal solid waste landfill leachate by flame atomic absorption spectrometry (FAAS). Three variables (pH, irradiation time and buffer concentration) were optimized using Doehlert design and the proportions of mixture components submitted to UV radiation (leachate sample, buffer solution and H2O2 30%, v/v) were optimized using a constrained mixture design. Using the experimental conditions established, this procedure allows limits of detection of 0.075, 0.025, 0.010, 0.075 and 0.041 µg mL-1, and the precision levels expressed as relative standard (%RSD, 0.5 µg mL-1) were 3.6, 1.8, 1.3, 3.3 and 1.7%, for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Landfill leachate; Photo-oxidation decomposition; UV radiation; Metal determination; FAAS.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100002
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