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Registros recuperados: 32
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A high load of non-neutral amino-acid polymorphisms explains high protein diversity despite moderate effective population size in a marine bivalve with sweepstakes reproduction ArchiMer
Harrang, Estelle; Lapegue, Sylvie; Morga, Benjamin; Bierne, Nicolas.
Marine bivalves show among the greatest allozyme diversity ever reported in Eukaryotes, putting them historically at the heart of the neutralist-selectionist controversy on the maintenance of genetic variation. Although it is now acknowledged that this high diversity is most probably a simple consequence of a large population size, convincing support for this explanation would require a rigorous assessment of the silent nucleotide diversity in natural populations of marine bivalves, which has not yet been done. This study investigated DNA sequence polymorphism in a set of 37 nuclear loci in wild samples of the flat oyster Ostrea edulis. Silent diversity was found to be only moderate (0.7%), and there was no departure from demographic equilibrium under the...
Tipo: Text Palavras-chave: Nucleotide; Polymorphism; Marine bivalve; Deleterious; Mutations; Genetic load; Ostrea edulis.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00125/23610/21444.pdf
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A New Barrier to Dispersal Trapped Old Genetic Clines That Escaped the Easter Microplate Tension Zone of the Pacific Vent Mussels ArchiMer
Plouviez, Sophie; Faure, Baptiste; Le Guen, Dominique; Lallier, Francois; Bierne, Nicolas; Jollivet, Didier.
Comparative phylogeography of deep-sea hydrothermal vent species has uncovered several genetic breaks between populations inhabiting northern and southern latitudes of the East Pacific Rise. However, the geographic width and position of genetic clines are variable among species. In this report, we further characterize the position and strength of barriers to gene flow between populations of the deep-sea vent mussel Bathymodiolus thermophilus. Eight allozyme loci and DNA sequences of four nuclear genes were added to previously published sequences of the cytochrome c oxidase subunit I gene. Our data confirm the presence of two barriers to gene flow, one located at the Easter Microplate (between 21 degrees 33'S and 31 degrees S) recently described as a hybrid...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00185/29622/28006.pdf
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A single clonal lineage of transmissible cancer identified in two marine mussel species in South America and Europe ArchiMer
Yonemitsu, Marisa A; Giersch, Rachael M; Polo-prieto, Maria; Hammel, Maurine; Simon, Alexis; Cremonte, Florencia; Avilés, Fernando T; Merino-véliz, Nicolás; Burioli, Erika Av; Muttray, Annette F; Sherry, James; Reinisch, Carol; Baldwin, Susan A; Goff, Stephen P; Houssin, Maryline; Arriagada, Gloria; Vázquez, Nuria; Bierne, Nicolas; Metzger, Michael J.
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00590/70255/68304.pdf
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Additive transcriptomic variation associated with reproductive traits suggest local adaptation in a recently settled population of the Pacific oyster, Crassostrea gigas ArchiMer
Sussarellu, Rossana; Huvet, Arnaud; Lapegue, Sylvie; Quillen, Virgile; Lelong, Christophe; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre.
Background Originating from Northeast Asia, the Pacific oyster Crassostrea gigas has been introduced into a large number of countries for aquaculture purpose. Following introduction, the Pacific oyster has turned into an invasive species in an increasing number of coastal areas, notably recently in Northern Europe. Methods To explore potential adaptation of reproductive traits in populations with different histories, we set up a common garden experiment based on the comparison of progenies from two populations of Pacific oyster sampled in France and Denmark and their hybrids. Sex ratio, condition index and microarray gene expression in gonads, were analyzed in each progeny (n = 60). Results A female-biased sex-ratio and a higher condition index were...
Tipo: Text Palavras-chave: Crassostrea gigas; Adaptation; Invasiveness; Fertility; Sex-ratio; Transcriptome; Microarray.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00287/39829/38343.pdf
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Analysis of genome-wide differentiation between native and introduced populations of the cupped oysters Crassostrea gigas and Crassostrea angulata ArchiMer
Gagnaire, Pierre-alexandre; Lamy, Jean-baptiste; Cornette, Florence; Heurtebise, Serge; Degremont, Lionel; Flahauw, Emilie; Boudry, Pierre; Bierne, Nicolas; Lapegue, Sylvie.
The Pacific cupped oyster is genetically subdivided into two sister taxa, Crassostrea gigas and C. angulata, which are in contact in the north-western Pacific. The nature and origin of their genetic and taxonomic differentiation remains controversial due the lack of known reproductive barriers and the high degree of morphologic similarity. In particular, whether the presence of ecological and/or intrinsic isolating mechanisms contributes to species divergence is unknown. The recent co-introduction of both taxa into Europe offers a unique opportunity to test how genetic differentiation is maintained under new environmental and demographic conditions. We generated a pseudo-chromosome assembly of the Pacific oyster genome using a combination of BAC-end...
Tipo: Text Palavras-chave: Cupped oysters; Genome assembly; Species divergence; Reproductive barriers; Recombination rate.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00455/56622/58337.pdf
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Application of high-throughput sequencing to population differentiation in the cupped oysters Crassostrea angulata/C. gigas ArchiMer
Gagnaire, Pierre-alexandre; Cornette, Florence; Heurtebise, Serge; Auge, M. T.; Bierne, Nicolas; Lapegue, Sylvie.
The cupped oysters Crassostrea angulata and Crassostrea gigas have been sequentially introduced in Europe from their native Pacific area for aquaculture purposes. Ongoing viral epidemic disease is responsible for large losses in oyster aquaculture production throughout Europe. In parallel with current efforts in developing selection programs for disease resistance, it becomes critically important to characterize genetic diversity patterns in both native and introduced areas. With the advent of next generation sequencing techniques, the power to resolve the fine-scale genetic structure at a genome-wide scale opens to new perspectives towards genetic resource management. Here, we present a genome-wide screen of genetic variation that combines SNP genotyping...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27816/26009.pdf
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Assortative fertilization and selection at larval stage in the mussels Mytilus edulis and M-galloprovincialis ArchiMer
Bierne, Nicolas; David, Pierre; Boudry, Pierre; Bonhomme, F.
Assortative mating (prezygotic isolation) and reduced hybrid fitness (postzygotic isolation) are typically invoked to explain the stability of hybrid zones. In the tension zone model. these factors work in opposition to migration, which promotes genetic homogeneity. Many marine animals migrate over long distances through a planktonic larval stage. Therefore. strong reproductive isolation is needed to maintain stable marine hybrid zones. However, surprisingly little is known about mating preferences and hybrid fitness in marine organisms. Smooth-shelled mussels (Mytilus spp.) form a well-known species complex, with hybridization over extensive areas such as the contact zone of M. edulis and M. galloprovincialis around European Atlantic coasts. This paper...
Tipo: Text Palavras-chave: Preferential fertilization; Mytilus galloprovincialis; Mytilus edulis; Hybrid viability; Heterosis; Gamete competition.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-2781.pdf
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"Bivalvomix" : génomique évolutive des bivalves marins ArchiMer
Bierne, Nicolas; Boudry, Pierre; Lapegue, Sylvie; Bonhomme, Francois; Faure, M; Sauvage, Christopher; Moraga, D; Boutet, Isabelle; David, Elise; Jollivet, Didier; Tanguy, Arnaud; Faure, Baptiste; David, Patrice.
For a long time, population geneticists have had to content themselves with analysing neutral markers to infer selection processes indirectly. The recent improvement of sequencing tools now enables them to analyse the variations of a large number of genes and therefore to spot the genes, or the amino acids, which are under the direct influence of the selection (Yang & Bielawski, 2000). In order to analyse the selection processes acting on the mutations and discriminate the different factors acting on their evolution (genetic selection or drift), it is essential to compare polymorphism data between genomes of the same species/population and divergence data between species/populations (Kimura, 1983). Recently, polymorphism data has become available for a...
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Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/rapport-6489.pdf
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Can habitat specialisation maintain a mosaic hybrid zone in marine bivalves? ArchiMer
Bierne, Nicolas; David, Pierre; Langlade, Aime; Bonhomme, F.
Maintaining the integrity of differentiated genomes in marine organisms needs efficient isolation mechanisms, because planktonic larval dispersion provides contacts between taxa. Habitat specialisation is interesting in this respect, because it can both prevent interspecific crosses (each taxon reproduces in its own habitat) and eliminate hybrids (typically less fit than a parental taxon in each habitat). The contact zone between smooth-shelled mussels Mytilus edulis and M. galloprovincialis in Europe is a good example, as allozyme genotypes typical of both taxa seem to segregate into different habitats. However, allozymes may be selected directly and it is not known whether the same pattern can be extended to the whole genome. Here, we used 6 presumably...
Tipo: Text Palavras-chave: Habitat specialisation; Hybrid zone; Introgression; Mytilus edulis; Mytilus galloprovincialis.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00000/10228/7597.pdf
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Comprendre et inférer la connectivité marine pour mieux gérer les ressources ArchiMer
Bierne, Nicolas; Arnaud-haond, Sophie.
Tipo: Text
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00487/59820/62963.pdf
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Dedicated population genomics for the silent world: the specific questions of marine population genetics ArchiMer
Bierne, Nicolas; Bonhomme, Francois; Arnaud-haond, Sophie.
Tipo: Text
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00374/48545/48841.pdf
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Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas) ArchiMer
Sauvage, Christopher; Lapegue, Sylvie; Bierne, Nicolas; Boudry, Pierre.
Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). The identification of markers associated to the resistance would allow to better understand this complex phenomenon and, eventually to establish markers assisted selection. Recently, two moderately dense genetic linkage maps have been established in the Pacific oyster based on microsatellites and AFLP markers (Hubert and Hedgecock, 2004; Li and Guo, 2004). In this context, the development and mapping of SNPs will increase the density of the linkage map and consequently the...
Tipo: Text Palavras-chave: QTLs analyses; Summer mortaliy; Genetic; SNPs; Crassostrea gigas; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3351.pdf
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Exploring local reproductive differentiation of Pacific oyster population recently settled in northern Europe by transcriptomic approach ArchiMer
Sussarellu, Rossana; Huvet, Arnaud; Lelong, Christophe; Pernet, Fabrice; Quillen, Virgile; Lapegue, Sylvie; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre.
Originating from north-eastern Asia, the Pacific oyster Crassostrea gigas has been introduced, mainly for aquaculture purpose, into a large number of countries. Although highly variable, its invasive nature has been reported in an increasing number of coastal areas, notably. Northern Europe. We set up a common garden experiment based on the comparison of progenies of Pacific oysters sampled in France, where populations settled since the introduction of the species in the 70’s, and Denmark, where established populations were observed since the beginning of 90s. Pure and hybrid progenies, resulting from crosses within and between adults sampled in both populations, were studied for their sex-ratio, condition index, transcriptomics, and biochemical parameters
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27815/26008.pdf
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Fitness landscapes support the dominance theory of post-zygotic isolation in the mussels Mytilus edulis and M. galloprovincialis ArchiMer
Bierne, Nicolas; Bonhomme, Francois; Boudry, Pierre; Szulkin, M; David, Pierre.
We studied the genetic basis of post-zygotic isolation in the marine mussels Mytilus edulis and Mytilus galloprovincialis. Evidence was obtained for a high number of recessive Dobzhansky-Muller substitutions in the genome of these two mussel taxa. We analysed the segregation of unlinked diagnostic markers in the progeny of two backcrosses and an F-2 cross, 36 h and 200 days after fertilization. Directional selection favouring M. galloprovincialis genotypes was observed in both kinds of cross. In the F-2, epistatic interactions between each pair of chromosome fragments mapped by the markers were identified in addition. Our results imply that homozygous-homozygous interactions are required for breakdown of coadaptation, in accordance with the dominance...
Tipo: Text Palavras-chave: Mytilus; Dobzhansky Muller incopatibilities; Post zygotic isolation; Negative epistasis; Hybrid breakdown; Hybrid zone.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1350.pdf
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Fragmentation des populations naturelles d'Ostrea edulis: une adaptation locale de l'huître plate européenne ArchiMer
Harrang, Estelle; Bierne, Nicolas; Heurtebise, Serge; Morga, Benjamin; Lapegue, Sylvie.
L'huître plate européenne Ostrea edulis a connu une période faste dans l'aquaculture jusqu'à la fin des années 1960, lorsque deux parasites, Marteilia refringens (1969) et Bonamia ostreae (1979), ont successivement décimé les bancs naturels de cette espèce endémique, déjà affaiblis par la sur-exploitation de ses gisements. Historiquement présente sur toute la côte européenne, de la Mer du Nord à la Mer Méditerranée, en passant par l'Océan Atlantique, la Mer Adriatique et la Mer Noire, les bancs naturels de cette espèce sont devenus très localisés, laissant penser à des phénomènes d'adaptation locale de l'huître vis à vis de son environnement biotique et abiotique. La diversité et la structuration génétique de plusieurs populations de l'espèce, à l'échelle...
Tipo: Text Palavras-chave: Huître plate; Ostrea edulis; Génétique des populations; Sélection Génétique.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12547/9417.pdf
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Génomique de l'adaptation de l'huître creuse, Crassostrea gigas, dans le contexte de sa récente expansion géographique en Europe ArchiMer
Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie.
Originaire du nord de l’Asie, l'huître creuse, Crassostrea gigas, a été introduite et transférée, principalement pour l’aquaculture, dans plusieurs pays européens depuis le début des années 1970. Le caractère invasif de cette espèce a été observé au cours de la dernière décennie dans plusieurs pays et elle a même été considérée comme une espèce nuisible dans certaines régions, telles que la mer de Wadden. Dans le cadre du projet HiFlo, soutenu par l’ANR, nous avons pour objectif d'identifier les caractéristiques d'une telle espèce florissante: son succès peut-il être expliqué par le hasard des opportunités (expansion de leur niche écologique) ou est-il lié à des processus évolutifs, ce qui impliquerait une adaptation génétique de l'espèce à de nouvelles...
Tipo: Text Palavras-chave: Huîtres; Crassostrea gigas; Génétique; Génétique des populations.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12552/9418.pdf
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Génomique de l'adaptation de l'huître creuse du Pacifique, Crassostrea gigas, dans le cadre de son expansion géographique ArchiMer
Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3). Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text Palavras-chave: Pacific oyster; Invasive; AFLP; Genome scan; Crassostrea gigas.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-4542.pdf
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Large-scale genetic panmixia in the blue shark ( Prionace glauca ): a single worldwide population, or a genetic lag-time effect of the “grey zone” of differentiation? ArchiMer
Bailleul, Diane; Mackenzie, Alicia; Sacchi, Olivier; Poisson, Francois; Bierne, Nicolas; Arnaud-haond, Sophie.
The blue shark Prionace glauca, among the most common and widely studied pelagic sharks, is a top predator, exhibiting the widest distribution range. However, little is known about its population structure and spatial dynamics. With an estimated removal of 10 to 20 million individuals per year by fisheries, the species is classified as “Near Threatened” by International Union for Conservation of Nature. We lack the knowledge to forecast the long-term consequences of such a huge removal on this top predator itself and on its trophic network. The genetic analysis of more than 200 samples collected at broad scale (from Mediterranean Sea, North Atlantic and Pacific Oceans) using mtDNA and nine microsatellite markers allowed to detect signatures of genetic...
Tipo: Text Palavras-chave: Blue shark; Conservation; Fisheries; Genetic panmixia; Prionace glauca; Stock.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00417/52866/53776.pdf
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Mark-recapture cloning: a straightforward and cost-effective cloning method for population genetics of single-copy nuclear DNA sequences in diploids ArchiMer
Bierne, Nicolas; Tanguy, A; Faure, Michel; Faure, B; David, E; Boutet, I; Boon, E; Quere, N; Plouviez, S; Kemppainen, P; Jollivet, D; Moraga, D; Boudry, Pierre; David, P.
We describe a simple protocol to reduce the number of cloning reactions of nuclear DNA sequences in population genetic studies of diploid organisms. Cloning is a necessary step to obtain correct haplotypes in such organisms, and, while traditional methods are efficient at cloning together many genes of a single individual, population geneticists rather need to clone the same locus in many individuals. Our method consists of marking individual sequences during the polymerase chain reaction (PCR) using 5'-tailed primers with small polynucleotide tags. PCR products are mixed together before the cloning reaction and clones are sequenced with universal plasmid primers. The individual from which a sequence comes from is identified by the tag sequences upstream...
Tipo: Text Palavras-chave: Sequence; Population genetics; High throughput allele recognition; DNA polymorphism.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4222.pdf
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Microsatellite analysis of 6-hour-old embryos reveals no preferential intraspecific fertilization between cupped oysters Crassostrea gigas and Crassostrea angulata ArchiMer
Huvet, Arnaud; Balabaud, K; Bierne, Nicolas; Boudry, Pierre.
Experimental examination of reproductive isolation is the first step in understanding li,hybridization processes. Here, we studied preferential fertilization between 2 cupped oyster taxa, Crassostrea angulata and Crassostrea gigas, as a potential prezygotic reproductive isolation. Early examination of sperm competition is now possible by molecular analysis of oyster embryos. This avoids the confounding effect of differential mortality during the larval stage. Six hundred embryos were sampled from 2 crosses. Three microsatellite loci were enough to determine without ambiguity the taxa of contributing sires of embryos. No evidence of preferential fertilization between gametes from the same taxa was shown. A significantly higher contribution of the C. gigas...
Tipo: Text Palavras-chave: Paternal contribution; Microsatellites; In vitro crossings; Hybridization; Crassostrea angulata; Crassostrea gigas.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-436.pdf
Registros recuperados: 32
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